Mouse Osteoprotegerin/TNFRSF11B Antibody
Mouse Osteoprotegerin/TNFRSF11B Antibody Summary
Glu22-Leu401 with a Gln138Arg substitution
Accession # Q6PI12
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
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Osteoprotegerin/TNFRSF11B Inhibition of TRAIL/TNFSF10-induced Cytotoxicity and Neutralization by Mouse Osteoprotegerin/TNFRSF11B Antibody. In the presence of the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Mouse OPG/TNFRSF11B Fc Chimera (Catalog # 459-MO) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, inhibition of Recombinant Human TRAIL/TNFSF10 (20 ng/mL) activity elicited by Recombinant Mouse OPG/TNFRSF11B Fc Chimera (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse OPG/TNFRSF11B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF459). The ND50 is typically 0.15-0.9 µg/mL.
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Osteoprotegerin/TNFRSF11B in Mouse Stomach. Osteoprotegerin/TNFRSF11B was detected in perfusion fixed frozen sections of mouse stomach using Goat Anti-Mouse Osteoprotegerin/TNFRSF11B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF459) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Osteoprotegerin/TNFRSF11B
Osteoprotegerin (OPG)/Osteoclastogenesis Inhibitory Factor (OCIF) is member of the tumor necrosis factor receptor superfamily that lacks any apparent cell-association motifs and exists as a soluble secreted protein. In the new TNF superfamily nomenclature, OPG is referred to as TNFRSF11B. OPG was originally isolated by sequence homology as a TNF receptor family protein during a fetal rat intestine cDNA-sequencing project and subsequently shown to be involved in the regulation of bone density. OCIF was initially purified from the conditioned medium of human embryonic fibroblasts based on its ability to inhibit osteoclast development. Comparison of the amino-acid sequences of human OPG and OCIF proteins revealed their identity. The amino-terminal half of OPG contains four cysteine-rich repeats characteristic of TNF receptor family members. The carboxy-terminal of OPG/OCIF was found to contain two death domain homologous regions in tandem. Human and mouse OPG share approximately 84% and 94% amino acid sequence identity, respectively, with the rat OPG. Natural OPG/OCIF has been found to exist predominantly as disulfide-linked dimers. Two TNF superfamily ligands, including the membrane proteins OPG ligand/TRANCE (tumor necrosis factor-related activation-induced cytokine)/ODF (osteoclast differentiation factor)/RANKL (receptor activator of NF-kappaB ligand) and TRAIL (TNF-related apoptosis-inducing ligand)/APO-2 ligand, have been shown to be the cellular ligands for OPG/OCIF. Each of these ligands has been shown to interact with additional TNF receptor family members, including RANK (with TRANCE) and TRAIL receptors 1-4 (with TRAIL). The roles of these receptor-ligands in osteoclastogenesis, apoptosis and in the immune system remains to be elucidated.
- Lacey, D.L. et al. (1998) Cell 93:165.
- Emery, J.G. et al. (1998) J. Biol. Chem. 273:14363.
- Yasuda, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3597.
Product Datasheets
Citations for Mouse Osteoprotegerin/TNFRSF11B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Antiresorptive activity of osteoprotegerin requires an intact heparan sulfate-binding site
Authors: M Li, D Xu
Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Role of osteoprotegerin in the regulation of dental epithelial?mesenchymal signaling during tooth development
Authors: X Gao, J Zheng, S Tu, B Cai, R Zeng, L Xiang
Mol Med Rep, 2019;20(4):3035-3042.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
A therapeutic antibody targeting osteoprotegerin attenuates severe experimental pulmonary arterial hypertension
Authors: ND Arnold, JA Pickworth, LE West, S Dawson, JA Carvalho, H Casbolt, AT Braithwait, J Iremonger, L Renshall, V Germaschew, M McCourt, P Bland-Ward, H Kowash, AG Hameed, AMK Rothman, MG Frid, AA Roger Thom, HR Evans, M Southwood, NW Morrell, DC Crossman, MKB Whyte, KR Stenmark, CM Newman, DG Kiely, SE Francis, A Lawrie
Nat Commun, 2019;10(1):5183.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Dimerization Interface of Osteoprotegerin Revealed by Hydrogen-deuterium Exchange Mass Spectrometry
Authors: Y Xiao, M Li, R Larocque, F Zhang, A Malhotra, J Chen, RJ Linhardt, L Konermann, D Xu
J. Biol. Chem., 2018;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Morc3 mutant mice exhibit reduced cortical area and thickness, accompanied by altered haematopoietic stem cells niche and bone cell differentiation
Sci Rep, 2016;6(0):25964.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
TRAIL stimulates proliferation of vascular smooth muscle cells via activation of NF-kappaB and induction of insulin-like growth factor-1 receptor.
Authors: Kavurma MM, Schoppet M, Bobryshev YV, Khachigian LM, Bennett MR
J. Biol. Chem., 2008;283(12):7754-62.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
JunB as a downstream mediator of PTHrP actions in cementoblasts.
Authors: Berry JE, Ealba EL, Pettway GJ, Datta NS, Swanson EC, Somerman MJ, McCauley LK
J. Bone Miner. Res., 2005;21(2):246-57.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Bacteria induce osteoclastogenesis via an osteoblast-independent pathway.
Authors: Jiang Y, Mehta CK, Hsu TY, Alsulaimani FF
Infect. Immun., 2002;70(6):3143-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Estrogen inhibition of PTH-stimulated osteoclast formation and attachment in vitro: involvement of both PKA and PKC.
Authors: Liu BY, 2019, Wu PW, Bringhurst FR, Wang JT
141-147, 2002;143(2):627-35.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
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Reviews for Mouse Osteoprotegerin/TNFRSF11B Antibody
Average Rating: 4.3 (Based on 3 Reviews)
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AF459 used at 0.4 µg/ml, recombinant OPG used at from 200 ng/ml to 1 µg/ml.
