Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Neutralization

Cited:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Osteoprotegerin/TNFRSF11B Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Osteoprotegerin/TNFRSF11B
Glu22-Leu401 with a Gln138Arg substitution
Accession # Q6PI12

Specificity

Detects mouse Osteoprotegerin in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Osteoprotegerin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse Osteoprotegerin/TNFRSF11B Antibody

Osteoprotegerin/TNFRSF11B Inhibition of TRAIL/TNFSF10-induced Cytotoxicity and Neutralization by Mouse Osteoprotegerin/TNFRSF11B Antibody.

Osteoprotegerin/TNFRSF11B Inhibition of TRAIL/TNFSF10-induced Cytotoxicity and Neutralization by Mouse Osteoprotegerin/TNFRSF11B Antibody.

In the presence of the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Mouse OPG/TNFRSF11B Fc Chimera (Catalog # 459-MO) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, inhibition of Recombinant Human TRAIL/TNFSF10 (20 ng/mL) activity elicited by Recombinant Mouse OPG/TNFRSF11B Fc Chimera (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse OPG/TNFRSF11B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF459). The ND50 is typically 0.15-0.9 µg/mL.
Osteoprotegerin/TNFRSF11B antibody in Mouse Stomach by Immunohistochemistry (IHC-Fr).

Osteoprotegerin/TNFRSF11B in Mouse Stomach.

Osteoprotegerin/TNFRSF11B was detected in perfusion fixed frozen sections of mouse stomach using Goat Anti-Mouse Osteoprotegerin/TNFRSF11B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF459) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse Osteoprotegerin/TNFRSF11B by Western Blot

Detection of Mouse Osteoprotegerin/TNFRSF11B by Western Blot

Cell-autonomous defect of Col1a1-Krm2 transgenic osteoblasts.(A) BrdU incorporation assays revealed a higher proliferation rate in primary calvarial osteoblast cultures from transgenic mice after 2 days of differentiation. (B) Von Kossa staining performed at 10 days of differentiation reveals reduced mineralization of osteoblasts from Col1a1-Krm2 mice (scale bars, 1 cm), despite higher protein content (given below). Values represent mean ± SD (n = 3). Asterisks indicate statistically significant differences. (C) Western Blot analysis of canonical Wnt signaling using primary osteoblasts from wildtype and transgenic mice following stimulation with Wnt3a for 30 minutes. (D) Western Blot analysis (left) and ELISA (right) showing decreased Opg levels in cellular extracts and conditioned medium of osteoblasts from Col1a1-Krm2 mice. (E) Quantitative RT-PCR (left) and ELISA (right) demonstrating that the reduced expression of Tnfrsf11b in bones of 6 weeks old Col1a1-Krm2 mice results in decreased Opg serum levels. All bars represent mean ± SD (n = 4). Asterisks indicate statistically significant differences. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/20436912), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Osteoprotegerin/TNFRSF11B by Western Blot

Detection of Mouse Osteoprotegerin/TNFRSF11B by Western Blot

Cell-autonomous defect of Col1a1-Krm2 transgenic osteoblasts.(A) BrdU incorporation assays revealed a higher proliferation rate in primary calvarial osteoblast cultures from transgenic mice after 2 days of differentiation. (B) Von Kossa staining performed at 10 days of differentiation reveals reduced mineralization of osteoblasts from Col1a1-Krm2 mice (scale bars, 1 cm), despite higher protein content (given below). Values represent mean ± SD (n = 3). Asterisks indicate statistically significant differences. (C) Western Blot analysis of canonical Wnt signaling using primary osteoblasts from wildtype and transgenic mice following stimulation with Wnt3a for 30 minutes. (D) Western Blot analysis (left) and ELISA (right) showing decreased Opg levels in cellular extracts and conditioned medium of osteoblasts from Col1a1-Krm2 mice. (E) Quantitative RT-PCR (left) and ELISA (right) demonstrating that the reduced expression of Tnfrsf11b in bones of 6 weeks old Col1a1-Krm2 mice results in decreased Opg serum levels. All bars represent mean ± SD (n = 4). Asterisks indicate statistically significant differences. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/20436912), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Osteoprotegerin/TNFRSF11B Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse stomach

Neutralization

Measured by its ability to neutralize Osteoprotegerin/TNFRSF11B-mediated inhibition of TRAIL/TNFSF10-induced cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.15-0.9 µg/mL in the presence of 0.1 µg/mL Recombinant Mouse Osteoprotegerin/TNFRSF11B Fc Chimera, 20 ng/mL Recombinant Human TRAIL, and 1 µg/mL actinomycin D.

Reviewed Applications

Read 4 reviews rated 4.5 using AF459 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Osteoprotegerin/TNFRSF11B

Osteoprotegerin (OPG)/Osteoclastogenesis Inhibitory Factor (OCIF) is member of the tumor necrosis factor receptor superfamily that lacks any apparent cell-association motifs and exists as a soluble secreted protein. In the new TNF superfamily nomenclature, OPG is referred to as TNFRSF11B. OPG was originally isolated by sequence homology as a TNF receptor family protein during a fetal rat intestine cDNA-sequencing project and subsequently shown to be involved in the regulation of bone density. OCIF was initially purified from the conditioned medium of human embryonic fibroblasts based on its ability to inhibit osteoclast development. Comparison of the amino-acid sequences of human OPG and OCIF proteins revealed their identity. The amino-terminal half of OPG contains four cysteine-rich repeats characteristic of TNF receptor family members. The carboxy-terminal of OPG/OCIF was found to contain two death domain homologous regions in tandem. Human and mouse OPG share approximately 84% and 94% amino acid sequence identity, respectively, with the rat OPG. Natural OPG/OCIF has been found to exist predominantly as disulfide-linked dimers. Two TNF superfamily ligands, including the membrane proteins OPG ligand/TRANCE (tumor necrosis factor-related activation-induced cytokine)/ODF (osteoclast differentiation factor)/RANKL (receptor activator of NF-kappaB ligand) and TRAIL (TNF-related apoptosis-inducing ligand)/APO-2 ligand, have been shown to be the cellular ligands for OPG/OCIF. Each of these ligands has been shown to interact with additional TNF receptor family members, including RANK (with TRANCE) and TRAIL receptors 1-4 (with TRAIL). The roles of these receptor-ligands in osteoclastogenesis, apoptosis and in the immune system remains to be elucidated.

References

  1. Lacey, D.L. et al. (1998) Cell 93:165.
  2. Emery, J.G. et al. (1998) J. Biol. Chem. 273:14363.
  3. Yasuda, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3597.

Alternate Names

OCIF, TNFRSF11B

Entrez Gene IDs

4982 (Human); 18383 (Mouse)

Gene Symbol

TNFRSF11B

UniProt

Q6PI12

Additional Osteoprotegerin/TNFRSF11B Products

Product Documents for Mouse Osteoprotegerin/TNFRSF11B Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Osteoprotegerin/TNFRSF11B Antibody

For research use only

Citations for Mouse Osteoprotegerin/TNFRSF11B Antibody

Customer Reviews for Mouse Osteoprotegerin/TNFRSF11B Antibody (4)

4.5 out of 5
4 Customer Ratings
5 Stars
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4 Stars
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3 Stars
25%
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Showing  1 - 4 of 4 reviews Showing All
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  • Mouse Osteoprotegerin/TNFRSF11B Antibody
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: L929
    Species: L929 cell line
    Verified Customer | Posted 04/07/2023
    Use OPG antibody to neutralize rOPG and improve TRAIL-induced apoptosis
    Mouse Osteoprotegerin/TNFRSF11B Antibody AF459
  • Mouse Osteoprotegerin/TNFRSF11B Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: BONE
    Species: Mouse
    Verified Customer | Posted 06/27/2021
    Mouse Osteoprotegerin/TNFRSF11B Antibody AF459
  • Mouse Osteoprotegerin/TNFRSF11B Antibody
    Name: Ding Xu
    Application: Flow Cytometry
    Sample Tested: Recombinant protein and MC3T3-E1 mouse preosteoblast cell line
    Species: Mouse
    Verified Customer | Posted 09/15/2017
    AF459 used at 0.4 µg/ml, recombinant OPG used at from 200 ng/ml to 1 µg/ml.
    Mouse Osteoprotegerin/TNFRSF11B Antibody AF459
  • Mouse Osteoprotegerin/TNFRSF11B Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Aortic smooth muscle cells
    Species: Mouse
    Verified Customer | Posted 06/19/2017
    Mouse Osteoprotegerin/TNFRSF11B Antibody AF459

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