Mouse Osteoprotegerin/TNFRSF11B Antibody

(5 citations)
(2 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Osteoprotegerin in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Osteoprotegerin is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Osteoprotegerin/TNFRSF11B
    Glu22-Leu401 with a Gln138Arg substitution
    Accession # Q6PI12
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Osteoprotegerin/TNFRSF11B Fc Chimera (Catalog # 459-MO)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize Osteoprotegerin/TNFRSF11B-mediated inhibition of TRAIL/TNFSF10-induced cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.5-2 µg/mL in the presence of 0.1 µg/mL Recombinant Mouse Osteoprotegerin/TNFRSF11B Fc Chimera, 20 ng/mL Recombinant Human TRAIL, and 1 µg/mL actinomycin D.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Osteoprotegerin/TNFRSF11B Inhibition of TRAIL/TNFSF10-induced Cytotoxicity and Neutralization by Mouse Osteoprotegerin/TNFRSF11B Antibody. In the presence of the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Mouse OPG/TNFRSF11B Fc Chimera (Catalog # 459-MO) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375‑TL) induced cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, inhibition of Recombinant Human TRAIL/TNFSF10 (20 ng/mL) activity elicited by Recombinant Mouse OPG/TNFRSF11B Fc Chimera (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse OPG/TNFRSF11B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF459). The ND50 is typically 0.5‑2 µg/mL.
Immunohistochemistry
Osteoprotegerin/TNFRSF11B in Mouse Stomach. Osteoprotegerin/TNFRSF11B was detected in perfusion fixed frozen sections of mouse stomach using Goat Anti-Mouse Osteoprotegerin/TNFRSF11B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF459) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Osteoprotegerin/TNFRSF11B

Osteoprotegerin (OPG)/Osteoclastogenesis Inhibitory Factor (OCIF) is member of the tumor necrosis factor receptor superfamily that lacks any apparent cell-association motifs and exists as a soluble secreted protein. In the new TNF superfamily nomenclature, OPG is referred to as TNFRSF11B. OPG was originally isolated by sequence homology as a TNF receptor family protein during a fetal rat intestine cDNA-sequencing project and subsequently shown to be involved in the regulation of bone density. OCIF was initially purified from the conditioned medium of human embryonic fibroblasts based on its ability to inhibit osteoclast development. Comparison of the amino-acid sequences of human OPG and OCIF proteins revealed their identity. The amino-terminal half of OPG contains four cysteine-rich repeats characteristic of TNF receptor family members. The carboxy-terminal of OPG/OCIF was found to contain two death domain homologous regions in tandem. Human and mouse OPG share approximately 84% and 94% amino acid sequence identity, respectively, with the rat OPG. Natural OPG/OCIF has been found to exist predominantly as disulfide-linked dimers. Two TNF superfamily ligands, including the membrane proteins OPG ligand/TRANCE (tumor necrosis factor-related activation-induced cytokine)/ODF (osteoclast differentiation factor)/RANKL (receptor activator of NF-kappaB ligand) and TRAIL (TNF-related apoptosis-inducing ligand)/APO-2 ligand, have been shown to be the cellular ligands for OPG/OCIF. Each of these ligands has been shown to interact with additional TNF receptor family members, including RANK (with TRANCE) and TRAIL receptors 1 - 4 (with TRAIL). The roles of these receptor-ligands in osteoclastogenesis, apoptosis and in the immune system remains to be elucidated.

  • References:
    1. Lacey, D.L. et al. (1998) Cell 93:165.
    2. Emery, J.G. et al. (1998) J. Biol. Chem. 273:14363.
    3. Yasuda, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3597.
  • Entrez Gene IDs:
    4982 (Human); 18383 (Mouse)
  • Alternate Names:
    OCIF; OCIFMGC29565; OPGtumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TNFRSF11B; TR1; tumor necrosis factor receptor superfamily, member 11b
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Morc3 mutant mice exhibit reduced cortical area and thickness, accompanied by altered haematopoietic stem cells niche and bone cell differentiation
    Sci Rep, 2016;6(0):25964.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. TRAIL stimulates proliferation of vascular smooth muscle cells via activation of NF-kappaB and induction of insulin-like growth factor-1 receptor.
    Authors: Kavurma MM, Schoppet M, Bobryshev YV, Khachigian LM, Bennett MR
    J. Biol. Chem., 2008;283(12):7754-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. JunB as a downstream mediator of PTHrP actions in cementoblasts.
    Authors: Berry JE, Ealba EL, Pettway GJ, Datta NS, Swanson EC, Somerman MJ, McCauley LK
    J. Bone Miner. Res., 2005;21(2):246-57.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. Bacteria induce osteoclastogenesis via an osteoblast-independent pathway.
    Authors: Jiang Y, Mehta CK, Hsu TY, Alsulaimani FF
    Infect. Immun., 2002;70(6):3143-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. Estrogen inhibition of PTH-stimulated osteoclast formation and attachment in vitro: involvement of both PKA and PKC.
    Authors: Liu BY, Wu PW, Bringhurst FR, Wang JT
    Endocrinology, 2002;143(2):627-35.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
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Isotype Controls
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Average Rating: 4 (Based on 2 reviews)

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We have 2 reviews tested in 2 applications: Flow Cytometry, Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Mouse Osteoprotegerin/TNFRSF11B Antibody AF459
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 Flow Mouse Anonymous 09/15/2017
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Flow Cytometry Mouse Osteoprotegerin/TNFRSF11B Antibody AF459
Flow Cytometry: Mouse Osteoprotegerin/TNFRSF11B Antibody [AF459]

Summary

ApplicationFlow Cytometry
Sample TestedRecombinant protein,MC3T3-E1 mouse preosteoblast cell line
SpeciesMouse

Other Experimental Details

Other Experimental DetailsAF459 used at 0.4 µg/ml, recombinant OPG used at from 200 ng/ml to 1 µg/ml.
Western Blot Mouse Osteoprotegerin/TNFRSF11B Antibody AF459
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 WB Mouse Anonymous 06/19/2017
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Western Blot Mouse Osteoprotegerin/TNFRSF11B Antibody AF459
Western Blot: Mouse Osteoprotegerin/TNFRSF11B Antibody [AF459]

Summary

ApplicationWestern Blot
Sample TestedAortic smooth muscle cells
SpeciesMouse

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