Mouse Osteoprotegerin/TNFRSF11B Antibody

Catalog # Availability Size / Price Qty
AF459
AF459-SP
Osteoprotegerin/TNFRSF11B Inhibition of TRAIL/TNFSF10-induced Cytotoxicity and Neutralization by Mouse Osteoprotegerin/TNFRSF11B Antibody.
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Product Details
Citations (9)
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Reviews (3)

Mouse Osteoprotegerin/TNFRSF11B Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Osteoprotegerin in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Osteoprotegerin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Osteoprotegerin/TNFRSF11B
Glu22-Leu401 with a Gln138Arg substitution
Accession # Q6PI12
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize Osteoprotegerin/TNFRSF11B-mediated inhibition of TRAIL/TNFSF10-induced cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.15-0.9 µg/mL in the presence of 0.1 µg/mL Recombinant Mouse Osteoprotegerin/TNFRSF11B Fc Chimera, 20 ng/mL Recombinant Human TRAIL, and 1 µg/mL actinomycin D.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Neutralization Osteoprotegerin/TNFRSF11B Inhibition of TRAIL/TNFSF10-induced Cytotoxicity and Neutralization by Mouse Osteoprotegerin/TNFRSF11B Antibody. View Larger

Osteoprotegerin/TNFRSF11B Inhibition of TRAIL/TNFSF10-induced Cytotoxicity and Neutralization by Mouse Osteoprotegerin/TNFRSF11B Antibody. In the presence of the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Mouse OPG/TNFRSF11B Fc Chimera (Catalog # 459-MO) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, inhibition of Recombinant Human TRAIL/TNFSF10 (20 ng/mL) activity elicited by Recombinant Mouse OPG/TNFRSF11B Fc Chimera (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse OPG/TNFRSF11B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF459). The ND50 is typically 0.15-0.9 µg/mL.

Immunohistochemistry Osteoprotegerin/TNFRSF11B in Mouse Stomach. View Larger

Osteoprotegerin/TNFRSF11B in Mouse Stomach. Osteoprotegerin/TNFRSF11B was detected in perfusion fixed frozen sections of mouse stomach using Goat Anti-Mouse Osteoprotegerin/TNFRSF11B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF459) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteoprotegerin/TNFRSF11B

Osteoprotegerin (OPG)/Osteoclastogenesis Inhibitory Factor (OCIF) is member of the tumor necrosis factor receptor superfamily that lacks any apparent cell-association motifs and exists as a soluble secreted protein. In the new TNF superfamily nomenclature, OPG is referred to as TNFRSF11B. OPG was originally isolated by sequence homology as a TNF receptor family protein during a fetal rat intestine cDNA-sequencing project and subsequently shown to be involved in the regulation of bone density. OCIF was initially purified from the conditioned medium of human embryonic fibroblasts based on its ability to inhibit osteoclast development. Comparison of the amino-acid sequences of human OPG and OCIF proteins revealed their identity. The amino-terminal half of OPG contains four cysteine-rich repeats characteristic of TNF receptor family members. The carboxy-terminal of OPG/OCIF was found to contain two death domain homologous regions in tandem. Human and mouse OPG share approximately 84% and 94% amino acid sequence identity, respectively, with the rat OPG. Natural OPG/OCIF has been found to exist predominantly as disulfide-linked dimers. Two TNF superfamily ligands, including the membrane proteins OPG ligand/TRANCE (tumor necrosis factor-related activation-induced cytokine)/ODF (osteoclast differentiation factor)/RANKL (receptor activator of NF-kappaB ligand) and TRAIL (TNF-related apoptosis-inducing ligand)/APO-2 ligand, have been shown to be the cellular ligands for OPG/OCIF. Each of these ligands has been shown to interact with additional TNF receptor family members, including RANK (with TRANCE) and TRAIL receptors 1-4 (with TRAIL). The roles of these receptor-ligands in osteoclastogenesis, apoptosis and in the immune system remains to be elucidated.

References
  1. Lacey, D.L. et al. (1998) Cell 93:165.
  2. Emery, J.G. et al. (1998) J. Biol. Chem. 273:14363.
  3. Yasuda, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3597.
Entrez Gene IDs
4982 (Human); 18383 (Mouse)
Alternate Names
OCIF; OCIFMGC29565; OPG; OPGtumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TNFRSF11B; TR1; tumor necrosis factor receptor superfamily, member 11b

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Citations for Mouse Osteoprotegerin/TNFRSF11B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Antiresorptive activity of osteoprotegerin requires an intact heparan sulfate-binding site
    Authors: M Li, D Xu
    Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Role of osteoprotegerin in the regulation of dental epithelial?mesenchymal signaling during tooth development
    Authors: X Gao, J Zheng, S Tu, B Cai, R Zeng, L Xiang
    Mol Med Rep, 2019;20(4):3035-3042.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. A therapeutic antibody targeting osteoprotegerin attenuates severe experimental pulmonary arterial hypertension
    Authors: ND Arnold, JA Pickworth, LE West, S Dawson, JA Carvalho, H Casbolt, AT Braithwait, J Iremonger, L Renshall, V Germaschew, M McCourt, P Bland-Ward, H Kowash, AG Hameed, AMK Rothman, MG Frid, AA Roger Thom, HR Evans, M Southwood, NW Morrell, DC Crossman, MKB Whyte, KR Stenmark, CM Newman, DG Kiely, SE Francis, A Lawrie
    Nat Commun, 2019;10(1):5183.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. Dimerization Interface of Osteoprotegerin Revealed by Hydrogen-deuterium Exchange Mass Spectrometry
    Authors: Y Xiao, M Li, R Larocque, F Zhang, A Malhotra, J Chen, RJ Linhardt, L Konermann, D Xu
    J. Biol. Chem., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Morc3 mutant mice exhibit reduced cortical area and thickness, accompanied by altered haematopoietic stem cells niche and bone cell differentiation
    Sci Rep, 2016;6(0):25964.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. TRAIL stimulates proliferation of vascular smooth muscle cells via activation of NF-kappaB and induction of insulin-like growth factor-1 receptor.
    Authors: Kavurma MM, Schoppet M, Bobryshev YV, Khachigian LM, Bennett MR
    J. Biol. Chem., 2008;283(12):7754-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. JunB as a downstream mediator of PTHrP actions in cementoblasts.
    Authors: Berry JE, Ealba EL, Pettway GJ, Datta NS, Swanson EC, Somerman MJ, McCauley LK
    J. Bone Miner. Res., 2005;21(2):246-57.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Bacteria induce osteoclastogenesis via an osteoblast-independent pathway.
    Authors: Jiang Y, Mehta CK, Hsu TY, Alsulaimani FF
    Infect. Immun., 2002;70(6):3143-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Estrogen inhibition of PTH-stimulated osteoclast formation and attachment in vitro: involvement of both PKA and PKC.
    Authors: Liu BY, 2019, Wu PW, Bringhurst FR, Wang JT
    141-147, 2002;143(2):627-35.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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Reviews for Mouse Osteoprotegerin/TNFRSF11B Antibody

Average Rating: 4.3 (Based on 3 Reviews)

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Mouse Osteoprotegerin/TNFRSF11B Antibody
By Anonymous on 06/27/2021
Application: IHC Sample Tested: BONE Species: Mouse

Mouse Osteoprotegerin/TNFRSF11B Antibody
By Ding Xu on 09/15/2017
Application: Flow Sample Tested: MC3T3-E1 mouse preosteoblast cell line,Recombinant protein Species: Mouse

AF459 used at 0.4 µg/ml, recombinant OPG used at from 200 ng/ml to 1 µg/ml.


Mouse Osteoprotegerin/TNFRSF11B Antibody
By Anonymous on 06/19/2017
Application: WB Sample Tested: Aortic smooth muscle cells Species: Mouse