Mouse PD-L2/B7-DC PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse PD-L2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human PD-L2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse PD-L2
    Accession # Q9WUL5
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of PD‑L2/B7‑DC in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse PD‑L2/B7‑DC PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1022P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PD-L2/B7-DC

Mouse Programmed Death Ligand 2 (PD-L2), also named B7DC, CD273, and Butyrophilin-like Protein, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1-4). Other family members include B7-1, B7-2, B7-H2, PD-L1 (B7-H1), and B7-H3. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share from 20-40% amino acid (aa) sequence identity. The cloned mouse PD-L2 cDNA encodes a 247 aa type I membrane precursor protein with a putative 20 aa signal peptide, a 199 aa extracellular region containing one V-like and one C-like Ig domain, a 23 aa transmembrane region, and only a 5 aa cytoplasmic domain. The extracellular domains of mouse and human PD-L2 share approximately 72% aa sequence identity. PD-L2 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L1. Mouse PD-L1 and PD-L2 share approximately 34% aa sequence identity and have similar functions. PD-L2 is constitutively expressed in lymphoid and non-lymphoid organs (1-4). The expression of PD-L2 is detected on endothelial cells, dendritic cells (DC), thymic epithelial cells, follicular, memory and regulatory B cells, macrophages and monocytes (1-5). PD-L2 expression is also upregulated in a variety of tumor cell lines. On previously activated T cells, PD-L2 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a co-stimulatory function for the PD-1 ligands on resting T cells has also been reported. On DC, PD-L2 may promote a Th1 response, while on follicular B cells, PD-L2 would appear to regulate plasma cell and memory B cell generation (6,7).

  • References:
    1. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
    2. Tseng, B.S-Y. et al. (2001) J. Exp. Med. 193:839.
    3. Dai, S. et al. (2014) Cell Immunol. 290:72.
    4. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
    5. Kamphorst, A.O. et al. (2013) Curr. Opin, Immunol. 25:381.
    6. Singh, A.K. et al. (2011) Allergy 66:155.
    7. Good-Jacobson, K.L. et al. (2010) Nat. Immunol. 11:535.
  • Long Name:
    Programmed Death-1 Ligand-2
  • Entrez Gene IDs:
    80380 (Human); 58205 (Mouse); 102146227 (Cynomolgus Monkey)
  • Alternate Names:
    B7-DC; bA574F11.2; Btdc; Butyrophilin B7-DC; Butyrophilin-like Protein; CD273 antigen; CD273; CD273PD-1 ligand 2; MGC142240; PD-1-ligand 2; PDCD1L2MGC142238; PDCD1LG2; PDL2; PDL2B7DC; PD-L2PDCD1 ligand 2; programmed cell death 1 ligand 2; Programmed death ligand 2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Goat IgG PE-conjugated Antibody

Ctrl IC108P 5  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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