Mouse PDGF-C Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse PDGF‑C in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human (rh) PDGF-CC is observed and less than 1% cross-reactivity with rhPDGF-AA, rhPDGF-BB, rhPDGF-D, and recombinant rat PDGF-AB is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse PDGF-CC
    Val235-Gly345
    Accession # Q8CI19
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse PDGF-C
  • Immunohistochemistry
    5-15 µg/mL
    Perfusion fixed frozen sections of mouse kidney
  • Neutralization
    Measured by its ability to neutralize PDGF‑CC-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Raines, E. W. et al. (1985) Methods Enzymol. 109:749. The Neutralization Dose (ND50) is typically 4-16 µg/mL in the presence of 0.8 µg/mL Recombinant Mouse PDGF‑CC.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by PDGF‑CC and Neutralization by Mouse PDGF‑C Antibody.

Recombinant Mouse PDGF‑CC stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by 0.8 µg/mL Recombinant Mouse PDGF‑CC is neutralized (green line) by increasing concentrations of Goat Anti-Mouse PDGF-C Antigen Affinity-purified Poly­clonal Antibody (Catalog # AF1447). The ND50 is typically 4‑16 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDGF-C
The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).
  • Long Name:
    Platelet-derived Growth Factor C
  • Entrez Gene IDs:
    56034 (Human); 54635 (Mouse)
  • Alternate Names:
    FALLOTEIN; PDGFC; PDGF-C; platelet derived growth factor C; platelet-derived growth factor C; SCDGFSpinal cord-derived growth factor; secretory growth factor-like protein; VEGF-E
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. PDGF-CC underlies resistance to VEGF-A inhibition and combinatorial targeting of both suppresses pathological angiogenesis more efficiently
    Authors: L Zheng, C Zhao, Y Du, X Lin, Y Jiang, C Lee, G Tian, J Mi, X Li, Q Chen, Z Ye, L Huang, S Wang, X Ren, L Xing, W Chen, D Huang, Z Gao, S Zhang, W Lu, Z Tang, B Wang, R Ju, X Li
    Oncotarget, 2016;7(47):77902-77915.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation.
    Authors: Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, Wang B, Wardega P, Zhang F, Dong L, Zhang Y, Zhang SZ, Ding H, Fariss RN, Becker KG, Lennartsson J, Nagai N, Cao Y, Li X
    J. Exp. Med., 2010;207(4):867-80.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. PDGF-C mediates the angiogenic and tumorigenic properties of fibroblasts associated with tumors refractory to anti-VEGF treatment.
    Authors: Crawford Y, Kasman I, Yu L, Zhong C, Wu X, Modrusan Z, Kaminker J, Ferrara N
    Cancer Cell, 2009;15(1):21-34.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Platelet-derived growth factor isoform expression in carbon tetrachloride-induced chronic liver injury.
    Authors: Borkham-Kamphorst E, Kovalenko E, van Roeyen CR, Gassler N, Bomble M, Ostendorf T, Floege J, Gressner AM, Weiskirchen R
    Lab. Invest., 2008;88(10):1090-100.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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