Mouse PDGF-C Antibody

Catalog # Availability Size / Price Qty
AF1447
AF1447-SP
Mouse PDGF-C Antibody in Data
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Product Details
Citations (4)
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Mouse PDGF-C Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PDGF‑C in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human (rh) PDGF-CC is observed and less than 1% cross-reactivity with rhPDGF-AA, rhPDGF-BB, rhPDGF-D, and recombinant rat PDGF-AB is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse PDGF-CC
Val235-Gly345
Accession # Q8CI19
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse PDGF-C
Immunohistochemistry
5-15 µg/mL
Perfusion fixed paraffin-embedded sections of mouse kidney
Neutralization
Measured by its ability to neutralize PDGF‑CC-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Raines, E. W. et al. (1985) Methods Enzymol. 109:749. The Neutralization Dose (ND50) is typically 4-16 µg/mL in the presence of 0.8 µg/mL Recombinant Mouse PDGF‑CC.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry View Larger

PDGF‑C in Mouse Kidney. PDGF‑C was detected in perfusion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse PDGF‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1447) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Neutralization Cell Proliferation Induced by PDGF‑CC and Neutralization by Mouse PDGF‑C Antibody. View Larger

Cell Proliferation Induced by PDGF‑CC and Neutralization by Mouse PDGF‑C Antibody. Recombinant Mouse PDGF‑CC stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by 0.8 µg/mL Recombinant Mouse PDGF‑CC is neutralized (green line) by increasing concentrations of Goat Anti-Mouse PDGF-C Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF1447). The ND50 is typically 4‑16 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-C

PDGF-C, also named spinal cord derived growth factor (SCDGF) and follotain, is a member of the PDGF family that binds to the PDGF receptor alpha alpha and alpha beta.

Long Name
Platelet-derived Growth Factor C
Entrez Gene IDs
56034 (Human); 54635 (Mouse)
Alternate Names
FALLOTEIN; PDGFC; PDGF-C; platelet derived growth factor C; platelet-derived growth factor C; SCDGFSpinal cord-derived growth factor; secretory growth factor-like protein; VEGF-E

Product Datasheets

Citations for Mouse PDGF-C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. PDGF-CC underlies resistance to VEGF-A inhibition and combinatorial targeting of both suppresses pathological angiogenesis more efficiently
    Authors: L Zheng, C Zhao, Y Du, X Lin, Y Jiang, C Lee, G Tian, J Mi, X Li, Q Chen, Z Ye, L Huang, S Wang, X Ren, L Xing, W Chen, D Huang, Z Gao, S Zhang, W Lu, Z Tang, B Wang, R Ju, X Li
    Oncotarget, 2016;7(47):77902-77915.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation.
    Authors: Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, Wang B, Wardega P, Zhang F, Dong L, Zhang Y, Zhang SZ, Ding H, Fariss RN, Becker KG, Lennartsson J, Nagai N, Cao Y, Li X
    J. Exp. Med., 2010;207(4):867-80.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. PDGF-C mediates the angiogenic and tumorigenic properties of fibroblasts associated with tumors refractory to anti-VEGF treatment.
    Authors: Crawford Y, Kasman I, Yu L, Zhong C, Wu X, Modrusan Z, Kaminker J, Ferrara N
    Cancer Cell, 2009;15(1):21-34.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Platelet-derived growth factor isoform expression in carbon tetrachloride-induced chronic liver injury.
    Authors: Borkham-Kamphorst E, Kovalenko E, van Roeyen CR, Gassler N, Bomble M, Ostendorf T, Floege J, Gressner AM, Weiskirchen R
    Lab. Invest., 2008;88(10):1090-100.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P

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