Detection of Mouse PEDF R/PNPLA2 by Western Blot.
Western blot shows lysates of mouse adipose tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse PEDF R/PNPLA2 Monoclonal Antibody (Catalog # MAB5365) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PEDF R/PNPLA2 at approximately 52 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 10.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PEDF R/PNPLA2
PEDF R (Pigment epithelium derived factor receptor; also known as ATGL, PNPLA2, and Desnutrin) is a 55 kDa member of the PNPLA family of proteins. It is expressed in adipocytes, hepatocytes, skeletal muscle, and pigment epithelium. PEDF R specifically hydrolyses triglycerides, releasing long-chain fatty acids. Mouse PEDF R is described as being a 486 amino acid (aa) type II transmembrane (TM) protein. Its TM segment is stated to span aa 9-29. Alternatively, it may have a hydrophobic lipid-association domain between aa 267-295. It contains a palatin domain (aa 10-179) with a conserved GxSxG enzymatic motif. There are multiple splice forms. Two show alternate start sites at Met57 and Met232, while two others show a deletion of aa 163-196 and aa 253-308, respectively. Over aa 162-253, mouse PEDF R shares 98% and 93% aa sequence identity with rat and human PEDF R, respectively.
Pigment Epithelium-derived Factor Receptor/Patatin-like Phospholipase Domain-containing Protein 2
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