Mouse Podocalyxin Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1556
Product Details
Citations (3)
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Mouse Podocalyxin Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Podocalyxin in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human (rh) Podocalyxin and rhEndoglycan is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
His22-Arg402
Accession # Q9R0M4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Podocalyxin
Flow Cytometry
2.5 µg/106 cells
D3 mouse embryonic stem cell line
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse liver and thymus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).

References
  1. Li, J. et al. (2001) DNA Seq. 12(5):407.
  2. Hara, T. et al. (1999) Immunity 11(5):567.
Entrez Gene IDs
5420 (Human); 27205 (Mouse)
Alternate Names
GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL

Product Datasheets

Citations for Mouse Podocalyxin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1
    Authors: J Long, DL Galvan, K Mise, YS Kanwar, L Li, N Poungvarin, PA Overbeek, BH Chang, FR Danesh
    J. Biol. Chem., 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Isolation
  2. CNTO 530 functions as a potent EPO mimetic via unique sustained effects on bone marrow proerythroblast pools.
    Authors: Sathyanarayana P, Houde E, Marshall D, Volk A, Makropoulos D, Emerson C, Pradeep A, Bugelski PJ, Wojchowski DM
    Blood, 2009;113(20):4955-62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Erythropoietin modulation of podocalyxin and a proposed erythroblast niche.
    Authors: Sathyanarayana P, Menon MP, Bogacheva O, Bogachev O, Niss K, Kapelle WS, Houde E, Fang J, Wojchowski DM
    Blood, 2007;110(2):509-18.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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