Mouse RAGE Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse RAGE in ELISAs and Western blots.
  • Source
    Monoclonal Rat IgG2A Clone # 175409
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse RAGE
    Gly23-Ala342
    Accession # NP_031451
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
    • Mouse RAGE Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse RAGE Antibody (Catalog # MAB11792)
  • ELISA Detection (Matched Antibody Pair)
    0.5-2.0 µg/mL 
    Mouse RAGE Biotinylated Antibody (Catalog # BAM11791)
  • ELISA Standard
     
    Recombinant Mouse RAGE Fc Chimera Protein, CF (Catalog # 1179-RG)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse RAGE by Western Blot.
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse RAGE Monoclonal Antibody (Catalog # MAB11792) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for RAGE and mature RAGE at approximately 50 kDa and 38 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RAGE

Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules (1). AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multiligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins (1, 2).

The mouse RAGE gene encodes a 403 amino acid (aa) residue type I transmembrane glycoprotein with a 22 aa signal peptide, a 319 aa extracellular domain containing a Ig-like V-type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain (3). The V-type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V-type domain or the cytoplasmic tail, are known (1, 4). RAGE is highly expressed in the embryonic central nervous system (5). In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes and hepatocytes (6). The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression (1, 7). RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses, and immune or inflammatory disorders (1).

  • References:
    1. Schmidt, A. et al. (2001) J. Clin. Invest. 108:949.
    2. Chavakis, T. et al. (2003) J. Exp. Med. 198:507.
    3. Renard, C. et al. (1997) Mol. Pharmacol. 52:54.
    4. Yonekura, H. et al. (2003) Biochem. J. 370:1097.
    5. Hori, O. et al. (1995) J. Biol. Chem. 270:25752.
    6. Brett, J. et al. (1993) Am. J. Pathol. 143:1699.
    7. Valencia, J.V. et al. (2004) Diabetes 53:743.
  • Long Name:
    Receptor for Advanced Glycation End Products
  • Entrez Gene IDs:
    177 (Human); 11596 (Mouse); 81722 (Rat)
  • Alternate Names:
    AGER; EC 2.7.11.22; MOK protein kinase; MOKMAPK/MAK/MRK overlapping kinase; RAGE; RAGE-1; RAGE1renal cell carcinoma antigen (MOK protein kinase); Renal tumor antigen 1; renal tumor antigen
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Receptor for advanced glycation end-products (RAGE) is an indicator of direct lung injury in models of experimental lung injury.
    Authors: Su X, Looney MR, Gupta N, Matthay MA
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(1):L1-5.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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