Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 773816
View all RAMP2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse RAMP2
Ser45-Val159
Accession # Q9WUP0

Specificity

Detects mouse RAMP2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human RAMP2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse RAMP2 Antibody

Detection of RAMP2 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry.

Detection of RAMP2 in bEnd.3 Mouse Cell Line by Flow Cytometry.

bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse RAMP2 Monoclonal Antibody (Catalog # MAB6500, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
RAMP2 antibody in Mouse Lung by Immunohistochemistry (IHC-Fr).

RAMP2 in Mouse Lung.

RAMP2 was detected in perfusion fixed frozen sections of mouse lung using Rat Anti-Mouse RAMP2 Monoclonal Antibody (Catalog # MAB6500) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse RAMP2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: bEnd.3 mouse endothelioma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse lung

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: RAMP2

RAMP2 (receptor activity modifying protein 2) is a 20 kDa member of the RAMP family of proteins. It is expressed on cardiomyocytes, vascular smooth muscle cells and endothelium and interacts with CRLR to form a receptor complex for adrenomedullin (AM). AM induces vasodilation on AM1 receptor expressing cells. Mature mouse RAMP2 is a 145 amino acid (aa) type I transmembrane glycoprotein that contains a 115 aa extracellular domain (ECD) (aa 45-159) and a nine aa cytoplasmic region. Although the ECD contains no typical structural motifs, based on human, aa 100-106 are critical for AM binding. Over aa 45-159, mouse RAMP2 shares 57% and 83% aa identity with human and rat RAMP2, respectively.

Long Name

Receptor Activity Modifying Protein 2

Alternate Names

calcitonin receptor-like receptor activity modifying protein 2, Calcitonin-receptor-like receptor activity-modifying protein 2, CRLR activity-modifying protein 2, receptor (calcitonin) activity modifying protein 2, receptor (G protein-coupled) activity modifying protein 2, receptor activity modifying protein 2, receptor activity-modifying protein 2, receptor-activity-modifying protein 2

Entrez Gene IDs

10266 (Human); 54409 (Mouse); 58966 (Rat)

Gene Symbol

RAMP2

UniProt

Q9WUP0 (Mouse)

Additional RAMP2 Products

Product Documents for Mouse RAMP2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse RAMP2 Antibody

For research use only

Citations for Mouse RAMP2 Antibody

Customer Reviews for Mouse RAMP2 Antibody

There are currently no reviews for this product. Be the first to review Mouse RAMP2 Antibody and earn rewards!

Have you used Mouse RAMP2 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies