Mouse RAMP2 Antibody

Catalog # Availability Size / Price Qty
MAB6500
MAB6500-SP

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Detection of RAMP2 in bEnd.3 Mouse Cell Line by Flow Cytometry.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Mouse RAMP2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse RAMP2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human RAMP2 is observed.
Source
Monoclonal Rat IgG2A Clone # 773816
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse RAMP2
Ser45-Val159
Accession # Q9WUP0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of RAMP2 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of RAMP2 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse RAMP2 Monoclonal Antibody (Catalog # MAB6500, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Immunohistochemistry RAMP2 antibody in Mouse Lung by Immunohistochemistry (IHC-Fr). View Larger

RAMP2 in Mouse Lung. RAMP2 was detected in perfusion fixed frozen sections of mouse lung using Rat Anti-Mouse RAMP2 Monoclonal Antibody (Catalog # MAB6500) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RAMP2

RAMP2 (receptor activity modifying protein 2) is a 20 kDa member of the RAMP family of proteins. It is expressed on cardiomyocytes, vascular smooth muscle cells and endothelium and interacts with CRLR to form a receptor complex for adrenomedullin (AM). AM induces vasodilation on AM1 receptor expressing cells. Mature mouse RAMP2 is a 145 amino acid (aa) type I transmembrane glycoprotein that contains a 115 aa extracellular domain (ECD) (aa 45-159) and a nine aa cytoplasmic region. Although the ECD contains no typical structural motifs, based on human, aa 100-106 are critical for AM binding. Over aa 45-159, mouse RAMP2 shares 57% and 83% aa identity with human and rat RAMP2, respectively.

Long Name
Receptor Activity Modifying Protein 2
Entrez Gene IDs
10266 (Human); 54409 (Mouse); 58966 (Rat)
Alternate Names
calcitonin receptor-like receptor activity modifying protein 2; Calcitonin-receptor-like receptor activity-modifying protein 2; CRLR activity-modifying protein 2; RAMP2; receptor (calcitonin) activity modifying protein 2; receptor (G protein-coupled) activity modifying protein 2; receptor activity modifying protein 2; receptor activity-modifying protein 2; receptor-activity-modifying protein 2

Product Datasheets

You must select a language.

x

Citation for Mouse RAMP2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Adrenomedullin Enhances Mouse Gustatory Nerve Responses to Sugars via T1R-Independent Sweet Taste Pathway
    Authors: Iwata, S;Yoshida, R;Takai, S;Sanematsu, K;Shigemura, N;Ninomiya, Y;
    Nutrients
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse RAMP2 Antibody

There are currently no reviews for this product. Be the first to review Mouse RAMP2 Antibody and earn rewards!

Have you used Mouse RAMP2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review