Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all BMP-15/GDF-9B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse BMP-15/GDF-9B
Gln268-Arg392
Accession # Q9Z0L4

Specificity

Detects mouse and rat BMP-15/GDF-9B in Western blots and recombinant mouse in direct ELISAs. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) BMP‑15 is observed and less than 1% cross‑reactivity with rhBMP-3 and rhBMP-10 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse/Rat BMP‑15/GDF‑9B Antibody

Detection of Rat BMP-15/GDF-9B antibody by Western Blot.

Detection of Rat BMP‑15/GDF‑9B by Western Blot.

Western blot shows lysates of rat ovary tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat BMP-15/GDF-9B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5917) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BMP-15/GDF-9B proprecursor at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse/Rat BMP‑15/GDF‑9B Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Rat ovary tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-15/GDF-9B

BMP-15 (Bone morphogenetic protein 15; also GDF-9B) is a 24-26 kDa member of the TGF-beta superfamily of proteins. It is expressed by late primary follicle oocytes, where it promotes the transition of preantral granulosa cells to cumulus cells, and later the expansion of cumulus cells. Mouse BMP-15 proprecursor is a 50-55 kDa, 367 amino acid (aa) glycoprotein. It is proteolytically cleaved to generate a 40 kDa prosegment (aa 26-267) plus a 24 kDa, 124 aa mature region (aa 268-392) that may be phosphorylated (on Ser7 of the mature molecule) and/or glycosylated. Secreted BMP-15 does not occur as a mature homodimer, but it does exist as a mature monomer, an uncleaved proprecursor, or as a noncovalent heterodimer composed of a cleaved mature region and its prosegment. The heterodimer may also form an oligomer. The BMP-15 prosegment reportedly forms a noncovalent heterodimer with 20 kDa mature GDF-9. Mature mouse BMP-15 shares 70% and 91% aa identity with human and rat BMP-15, respectively.

Long Name

Bone Morphogenetic Protein 15

Alternate Names

BMP15, GDF-9B

Entrez Gene IDs

9210 (Human); 12155 (Mouse); 59302 (Rat)

Gene Symbol

BMP15

UniProt

Q9Z0L4

Additional BMP-15/GDF-9B Products

Product Documents for Mouse/Rat BMP‑15/GDF‑9B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Rat BMP‑15/GDF‑9B Antibody

For research use only

Related Research Areas

BMP Family

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