Mouse/Rat CD39L1/ENTPD2 Antibody

Catalog #: AF5797
3 Citations Datasheet / COA / SDS

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AF5797
AF5797-SP

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Detection of Mouse and Rat CD39L1/ENTPD2 by Western Blot.

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat CD39L1/ENTPD2 Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse and rat CD39L1/ENTPD2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) CD39, rmCD39L2, and rmCD39L3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse CD39L1/ENTPD2
Cys26-Ser462
Accession # O55026

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse CD39L1/ENTPD2 (Catalog # 5797-EN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse and Rat CD39L1/ENTPD2 by Western Blot. Western blot shows lysates of mouse salivary gland tissue and rat placenta tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat CD39L1/ENTPD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5797) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD39L1/ENTPD2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD39L1/ENTPD2

CD39L1, also known as ENTPD2 and NTPDase2, is an ecto-nucleotidase belonging to the CD39 family. It is found on the surface of vascular adventitial cells and accessory vascular cells (1). CD39L1 is a Ca²⁺- and Mg²⁺-dependent enzyme that activates platelets by preferentially converting ATP to ADP (2). CD39L1 plays a role in regulating thrombosis and inflammation (3). It is considered to be a therapeutic target for thromboregulation and the treatment of vascular inflammation (2, 4).

References

Zimmermann, H. et al. 2000 Proceedings of the Second International Workshop on Ecto-ATPases and Related Ectonucleotidases:18.
Robson, S.C. et al. 2001 Drug Dev. Res. 53:193.
Marcus, A.J. et al. 2005 Semin. Thromb. Hemost. 31:234.
Sevigny, J. et al. 2002 Blood 99:2801.

Long Name

Ecto-Nucleoside Triphosphate Diphosphohydrolase 2

Entrez Gene IDs

954 (Human); 12496 (Mouse)

Alternate Names

CD39 antigen-like 1; CD39L1; CD39L1NTPDase 2; CD39-like-1; EC 3.6.1; EC 3.6.1.-; EC 3.6.1.5; Ecto-ATP diphosphohydrolase 2; Ecto-ATPase 2; Ecto-ATPase; Ecto-ATPDase 2; ectonucleoside triphosphate diphosphohydrolase 2; ENTPD2; NTPD2; NTPDase 2; NTPDase-2

Product Datasheets

Product Datasheet

COA

SDS

Citations for Mouse/Rat CD39L1/ENTPD2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Expression of Renin-Angiotensin System Components in the Taste Organ of Mice
Authors: N Shigemura, S Takai, F Hirose, R Yoshida, K Sanematsu, Y Ninomiya
Nutrients, 2019;11(9):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Role of P2XReceptor in Mouse Voiding Function
Authors: W Yu, WG Hill, SC Robson, ML Zeidel
Sci Rep, 2018;8(1):1838.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Expression and distribution of ectonucleotidases in mouse urinary bladder.
Authors: Yu W, Robson SC, Hill WG
PLoS ONE, 2011;6(4):e18704.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot