Mouse/Rat CD39L1/ENTPD2 Antibody

Catalog # Availability Size / Price Qty
AF5797
AF5797-SP
Detection of Mouse and Rat CD39L1/ENTPD2 by Western Blot.
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Citations (9)
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Mouse/Rat CD39L1/ENTPD2 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat CD39L1/ENTPD2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) CD39, rmCD39L2, and rmCD39L3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CD39L1/ENTPD2
Cys26-Ser462
Accession # O55026
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse CD39L1/ENTPD2 (Catalog # 5797-EN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat CD39L1/ENTPD2 antibody by Western Blot. View Larger

Detection of Mouse and Rat CD39L1/ENTPD2 by Western Blot. Western blot shows lysates of mouse salivary gland tissue and rat placenta tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat CD39L1/ENTPD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5797) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD39L1/ENTPD2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry/ Immunofluorescence Detection of Mouse CD39L1/ENTPD2 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse CD39L1/ENTPD2 by Immunocytochemistry/Immunofluorescence NTPDase2 immunostaining co-localize with vimentin, desmin, and PDGF beta receptor in mice bladder.Cryosections of mouse bladders were labeled with antibodies to vimentin (A. green), desmin (D. green), PDGF beta receptor (G. green), and NTPDase2 (B. E. H. red) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C. F. I). Merged signals of NTPDase2 and vimentin, desmin, and PDGF beta receptor are shown as yellow (C. F. I). White arrows indicate representative co-localization. White asterisks indicate non-co-localized signal of smooth muscle (I) and fibroblasts (F. I). White scale bars = 10 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23145014), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse CD39L1/ENTPD2 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse CD39L1/ENTPD2 by Immunocytochemistry/Immunofluorescence NTPDase2 co-localizes with CD34 but not tryptase in mouse bladder.Cryosections of mouse bladders were labeled with antibodies to CD34 (A, D. green), NTPDase2 (B. red), tryptase (E. red), c-kit (G. green), and Topro-3 to label nuclei (B. E. H. blue). Color merged panels are shown on the right (C, F, I). Merged signals are shown as yellow (C. F. I). White arrows indicate representative NTPDase2/CD34 co-localization (C) or CD34/tryptase co-localization (F); White asterisks indicate CD34 positive cells with no NTPDase2 staining (C). White scale bars = 10 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23145014), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse CD39L1/ENTPD2 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse CD39L1/ENTPD2 by Immunocytochemistry/Immunofluorescence NTPDase2 immunostaining co-localize with vimentin, desmin, and PDGF beta receptor in mice bladder.Cryosections of mouse bladders were labeled with antibodies to vimentin (A. green), desmin (D. green), PDGF beta receptor (G. green), and NTPDase2 (B. E. H. red) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C. F. I). Merged signals of NTPDase2 and vimentin, desmin, and PDGF beta receptor are shown as yellow (C. F. I). White arrows indicate representative co-localization. White asterisks indicate non-co-localized signal of smooth muscle (I) and fibroblasts (F. I). White scale bars = 10 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23145014), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse CD39L1/ENTPD2 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse CD39L1/ENTPD2 by Immunocytochemistry/Immunofluorescence NTPDase2 immunostaining does not co-localize with alpha -SMA in mice bladder smooth muscle.Cryosections of mouse bladders were labeled with antibodies to NTPDase2 (A. green), alpha -SMA (B. red) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C). White arrows indicate distinct NTPDase2 staining next to bladder smooth muscle cells. White scale bars = 10 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23145014), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse CD39L1/ENTPD2 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse CD39L1/ENTPD2 by Immunocytochemistry/Immunofluorescence NTPDase2 immunostaining co-localize with c-kit in mice bladder.Cryosections of mouse bladders were labeled with antibodies to NTPDase2 (B. red), c-kit (A. green) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C). Merged signals of NTPDase2 and c-kit are shown as yellow (C). White arrows indicate representative NTPDase2/c-kit co-localization. White scale bars = 10 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23145014), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse CD39L1/ENTPD2 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse CD39L1/ENTPD2 by Immunocytochemistry/Immunofluorescence NTPDase2 immunostaining co-localize with connexin 43 in mice bladder.Cryosections of mouse bladders were labeled with antibodies to connexin 43 (A. green), NTPDase2 (B. red) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C). Merged signals of NTPDase2 and connexin 43 are shown as yellow (C). White arrows indicate representative NTPDase2/connexin 43 co-localization. White scale bars = 10 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23145014), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse CD39L1/ENTPD2 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse CD39L1/ENTPD2 by Immunocytochemistry/Immunofluorescence NTPDase2 immunostaining co-localize with vimentin, desmin, and PDGF beta receptor in mice bladder.Cryosections of mouse bladders were labeled with antibodies to vimentin (A. green), desmin (D. green), PDGF beta receptor (G. green), and NTPDase2 (B. E. H. red) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C. F. I). Merged signals of NTPDase2 and vimentin, desmin, and PDGF beta receptor are shown as yellow (C. F. I). White arrows indicate representative co-localization. White asterisks indicate non-co-localized signal of smooth muscle (I) and fibroblasts (F. I). White scale bars = 10 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23145014), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse CD39L1/ENTPD2 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse CD39L1/ENTPD2 by Immunocytochemistry/Immunofluorescence NTPDase2 immunostaining co-localize with merlin/NF2 in mice bladder.Cryosections of mouse bladders were labeled with antibodies to merlin/NF2 (A. green), NTPDase2 (B. red) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C). Merged signals of NTPDase2 and merlin/NF2 are shown as yellow (C). White arrows indicate representative NTPDase2/merlin co-localization. White scale bars = 10 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23145014), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD39L1/ENTPD2

CD39L1, also known as ENTPD2 and NTPDase2, is an ecto-nucleotidase belonging to the CD39 family. It is found on the surface of vascular adventitial cells and accessory vascular cells (1). CD39L1 is a Ca2+- and Mg2+-dependent enzyme that activates platelets by preferentially converting ATP to ADP (2). CD39L1 plays a role in regulating thrombosis and inflammation (3). It is considered to be a therapeutic target for thromboregulation and the treatment of vascular inflammation (2, 4).

References
  1. Zimmermann, H. et al. 2000 Proceedings of the Second International Workshop on Ecto-ATPases and Related Ectonucleotidases:18.        
  2. Robson, S.C. et al. 2001 Drug Dev. Res. 53:193.  
  3. Marcus, A.J. et al. 2005 Semin. Thromb. Hemost. 31:234.  
  4. Sevigny, J. et al. 2002 Blood 99:2801.
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 2
Entrez Gene IDs
954 (Human); 12496 (Mouse)
Alternate Names
CD39 antigen-like 1; CD39L1; CD39L1NTPDase 2; CD39-like-1; EC 3.6.1; EC 3.6.1.-; EC 3.6.1.5; Ecto-ATP diphosphohydrolase 2; Ecto-ATPase 2; Ecto-ATPase; Ecto-ATPDase 2; ectonucleoside triphosphate diphosphohydrolase 2; ENTPD2; NTPD2; NTPDase 2; NTPDase-2

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Citations for Mouse/Rat CD39L1/ENTPD2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Expression profile of the zinc transporter ZnT3 in taste cells of rat circumvallate papillae and its role in zinc release, a potential mechanism for taste stimulation
    Authors: Kentaro Nishida, Saho Bansho, Akiko Ikukawa, Teruyo Kubota, Akihiro Ohishi, Kazuki Nagasawa
    European Journal of Histochemistry
  2. Expression of Renin-Angiotensin System Components in the Taste Organ of Mice
    Authors: N Shigemura, S Takai, F Hirose, R Yoshida, K Sanematsu, Y Ninomiya
    Nutrients, 2019-09-19;11(9):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. ATP metabolizing enzymes ENPP1, 2 and 3 are localized in sensory neurons of rat dorsal root ganglion
    Authors: Kentaro Nishida, Yuka Nomura, Kanako Kawamori, Akihiro Ohishi, Kazuki Nagasawa
    European Journal of Histochemistry
  4. Role of P2XReceptor in Mouse Voiding Function
    Authors: W Yu, WG Hill, SC Robson, ML Zeidel
    Sci Rep, 2018-01-30;8(1):1838.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Expression of Prostatic Acid Phosphatase in Rat Circumvallate Papillae
    Authors: Kentaro Nishida, Teruyo Kubota, Saki Matsumoto, Junki Kato, Yu Watanabe, Atsuko Yamamoto et al.
    PLOS ONE
  6. Oxaliplatin Alters Expression of T1R2 Receptor and Sensitivity to Sweet Taste in Rats
    Authors: Akihiro Ohishi, Kentaro Nishida, Yuri Yamanaka, Ai Miyata, Akiko Ikukawa, Miharu Yabu et al.
    Biological & Pharmaceutical Bulletin
  7. Lack of specificity shown by P2Y6 receptor antibodies
    Authors: Weiqun Yu, Warren G. Hill
    Naunyn-Schmiedeberg's Archives of Pharmacology
  8. Cellular Expression Profile for Interstitial Cells of Cajal in Bladder - A Cell Often Misidentified as Myocyte or Myofibroblast
    Authors: Weiqun Yu, Mark L. Zeidel, Warren G. Hill
    PLoS ONE
  9. Expression and distribution of ectonucleotidases in mouse urinary bladder.
    Authors: Yu W, Robson SC, Hill WG
    PLoS ONE, 2011-04-14;6(4):e18704.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot

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