Mouse/Rat GDF-15 Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean | 25.6 | 60 | 190 | 26.7 | 63.1 | 196 |
Standard Deviation | 0.69 | 1.42 | 5.49 | 1.62 | 2.84 | 10.7 |
CV% | 2.7 | 2.4 | 2.9 | 6.1 | 4.5 | 5.5 |
Recovery
The recovery of GDF-15 spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Mouse Cell Culture Media (n=4) | 102 | 91-110 |
Mouse EDTA Plasma (n=4) | 95 | 81-109 |
Mouse Heparin Plasma (n=4) | 95 | 87-104 |
Mouse Serum (n=4) | 91 | 82-101 |
Mouse Urine (n=4) | 97 | 88-106 |
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background: GDF-15
Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.





Citations for Mouse/Rat GDF-15 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
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Both male and female obese ZSF1 rats develop cardiac dysfunction in obesity-induced heart failure with preserved ejection fraction
Authors: ITN Nguyen, MM Brandt, J van de Wou, RWA van Drie, M Wesseling, MJ Cramer, SCA de Jager, D Merkus, DJ Duncker, C Cheng, JA Joles, MC Verhaar
PLoS ONE, 2020;15(5):e0232399.
Species: Rat
Sample Types: Plasma
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Exploring the In�Vivo Role of the Mitochondrial Calcium Uniporter in Brown Fat Bioenergetics
Authors: D Flicker, Y Sancak, E Mick, O Goldberger, VK Mootha
Cell Rep, 2019;27(5):1364-1375.e5.
Species: Mouse
Sample Types: Plasma
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Role of GDF15 in active lifestyle induced metabolic adaptations and acute exercise response in mice
Authors: CI Gil, M Ost, J Kasch, S Schumann, S Heider, S Klaus
Sci Rep, 2019;9(1):20120.
Species: Mouse
Sample Types: Plasma
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GDF15 deficiency promotes high fat diet-induced obesity in mice
Authors: T Tran, J Yang, J Gardner, Y Xiong
PLoS ONE, 2018;13(8):e0201584.
Species: Mouse
Sample Types: Serum
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Supraphysiologic Administration of GDF11 Induces Cachexia in Part by Upregulating GDF15
Authors: JE Jones, SM Cadena, C Gong, X Wang, Z Chen, SX Wang, C Vickers, H Chen, E Lach-Trifi, JR Hadcock, DJ Glass
Cell Rep, 2018;22(6):1522-1530.
Species: Mouse
Sample Types: Plasma
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Induction of Growth Differentiation Factor 15 in Skeletal Muscle of Old Taurine Transporter Knockout Mouse
Authors: T Ito, Y Nakanishi, N Yamaji, S Murakami, SW Schaffer
Biol. Pharm. Bull., 2018;41(3):435-439.
Species: Mouse
Sample Types: Serum
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Partial involvement of Nrf2 in skeletal muscle mitohormesis as an adaptive response to mitochondrial uncoupling
Authors: V Coleman, P Sa-Nguanmo, J Koenig, TJ Schulz, T Grune, S Klaus, AP Kipp, M Ost
Sci Rep, 2018;8(1):2446.
Species: Mouse
Sample Types: Plasma
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Fasting exacerbates hepatic growth differentiation factor 15 to promote fatty acid ?-oxidation and ketogenesis via activating XBP1 signaling in liver
Authors: M Zhang, W Sun, J Qian, Y Tang
Redox Biol, 2018;16(0):87-96.
Species: Mouse
Sample Types: Tissue Homogenates
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GDF15 is elevated in mice following retinal ganglion cell death and in glaucoma patients
Authors: N Ban, CJ Siegfried, JB Lin, YB Shui, J Sein, W Pita-Thoma, A Sene, A Santeford, M Gordon, R Lamb, Z Dong, SC Kelly, V Cavalli, J Yoshino, RS Apte
JCI Insight, 2017;2(9):.
Species: Mouse
Sample Types: Aqueous Humor
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Oral Treatment with the Ghrelin Receptor Agonist HM01 Attenuates Cachexia in Mice Bearing Colon-26 (C26) Tumors
Authors: FO Villars, C Pietra, C Giuliano, TA Lutz, T Riediger
Int J Mol Sci, 2017;18(5):.
Species: Mouse
Sample Types: Plasma
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Mitochonic Acid 5 (MA-5) Facilitates ATP Synthase Oligomerization and Cell Survival in Various Mitochondrial Diseases
Authors: T Matsuhashi, T Sato, SI Kanno, T Suzuki, A Matsuo, Y Oba, M Kikusato, E Ogasawara, T Kudo, K Suzuki, O Ohara, H Shimbo, F Nanto, H Yamaguchi, D Saigusa, Y Mukaiyama, A Watabe, K Kikuchi, H Shima, E Mishima, Y Akiyama, Y Oikawa, HO Hsin-Jung, Y Akiyama, C Suzuki, M Uematsu, M Ogata, N Kumagai, M Toyomizu, A Hozawa, N Mano, Y Owada, S Aiba, T Yanagisawa, Y Tomioka, S Kure, S Ito, K Nakada, KI Hayashi, H Osaka, T Abe
EBioMedicine, 2017;0(0):.
Species: Mouse
Sample Types: Serum
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Loss of Hepatic Mitochondrial Long-Chain Fatty Acid Oxidation Confers Resistance to Diet-Induced Obesity and Glucose Intolerance
Authors: J Lee, J Choi, ES Selen Alpe, L Zhao, T Hartung, S Scafidi, RC Riddle, MJ Wolfgang
Cell Rep, 2017;20(3):655-667.
Species: Mouse
Sample Types: Serum
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Ghrelin protects against renal damages induced by angiotensin-II via an antioxidative stress mechanism in mice.
Authors: Fujimura K, Wakino S, Minakuchi H, Hasegawa K, Hosoya K, Komatsu M, Kaneko Y, Shinozuka K, Washida N, Kanda T, Tokuyama H, Hayashi K, Itoh H
PLoS ONE, 2014;9(4):e94373.
Species: Mouse
Sample Types: Urine
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