Leptin, also known as OB, is a polypeptide hormone that regulates adipose tissue mass and energy balance. In mice (ob/ob) and humans, inactivating mutations of the OB gene can cause obesity. Leptin signals through the transmembrane Leptin R in the hypothalamus but can also bind shorter and soluble forms of Leptin R which do not signal. Adipocytes increase Leptin expression as cell size increases, resulting in depressed appetite and increased energy expenditure. Low Leptin levels prompt increased appetite and decreased energy expenditure. Leptin can also block the onset of puberty or menses during starvation, extreme exercise-induced weight loss, anorexia, or cancer-induced cachexia.
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Mouse/Rat Leptin Quantikine ELISA Kit
R&D Systems | Catalog # MOB00B
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Key Product Details
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Mouse Serum (10 µL), Rat Serum (25 µL), Mouse EDTA Plasma (10 µL), Rat EDTA Plasma (25 µL), Mouse Heparin Plasma (10 µL), Rat Heparin Plasma (25 µL)
Sensitivity
5.56 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Mouse Heparin Plasma, Rat Heparin Plasma)
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Product Summary for Mouse/Rat Leptin Quantikine ELISA Kit
The Quantikine® Mouse/Rat Leptin Immunoassay is a 4.0 hour solid phase ELISA designed to measure mouse or rat Leptin in cell culture supernates, tissue homogenates, serum, and plasma. It contains E.
coli-expressed recombinant mouse Leptin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse Leptin accurately. Results obtained for naturally occurring mouse or rat Leptin showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse or rat Leptin.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse, Rat
Specificity
Natural and recombinant mouse and rat Leptin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Serum, Rat EDTA Plasma, Rat Heparin Plasma, Rat Serum, Tissue Homogenates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 152 | 421 | 1512 | 143 | 389 | 1469 |
| Standard Deviation | 5.40 | 8.58 | 41.0 | 11.1 | 30.5 | 87.2 |
| CV% | 3.6 | 2.0 | 2.7 | 7.8 | 7.8 | 5.9 |
Recovery for Mouse/Rat Leptin Quantikine ELISA Kit
The recovery of mouse/rat Leptin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Mouse Cell Culture Media (n=4) | 109 | 98-117 |
| Mouse EDTA Plasma (n=4) | 96 | 89-110 |
| Mouse Heparin Plasma (n=4) | 90 | 80-105 |
| Mouse Serum (n=4) | 95 | 90-102 |
| Rat EDTA Plasma (n=4) | 106 | 99-113 |
| Rat Heparin Plasma (n=4) | 98 | 91-108 |
| Rat Serum (n=4) | 98 | 91-103 |
Linearity
To assess the linearity of the assay, four or more samples containing and/or spiked with high concentrations of mouse/rat Leptin in each matrix were diluted with calibrator diluent and assayed.
Scientific Data Images for Mouse/Rat Leptin Quantikine ELISA Kit
Mouse/Rat Leptin/OB ELISA Standard Curve
Mouse/Rat Leptin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Leptin/OB
Additional Leptin/OB Products
Product Documents for Mouse/Rat Leptin Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse/Rat Leptin Quantikine ELISA Kit
For research use only
Citations for Mouse/Rat Leptin Quantikine ELISA Kit
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Customer Reviews for Mouse/Rat Leptin Quantikine ELISA Kit (11)
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Sample Tested: mouse plasma/EDTA and EDTA PlasmaVerified Customer | Posted 06/23/2023Mice plasmas were diluted 1:50 and assayed. This kit works pretty well with zero background.
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Sample Tested: Serum and SerumVerified Customer | Posted 06/23/2023
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Sample Tested: SerumVerified Customer | Posted 10/27/2022
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Sample Tested: EDTA Plasma and SerumVerified Customer | Posted 09/21/2022I'd been using it a lot and works perfectly!
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Sample Tested: SerumVerified Customer | Posted 09/01/2022
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Sample Tested: SerumVerified Customer | Posted 07/15/2022
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Sample Tested: SerumVerified Customer | Posted 06/06/2022
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Sample Tested: SerumVerified Customer | Posted 01/14/2022
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Sample Tested: EDTA PlasmaVerified Customer | Posted 08/25/2021
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Sample Tested: SerumVerified Customer | Posted 04/05/2021
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Sample Tested: EDTA PlasmaVerified Customer | Posted 11/17/2020The performance of this analysis was great with samples in the log part of the standard curve.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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