Mouse/Rat PDGF-AB Quantikine ELISA Kit

  (8 citations)     
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Assay Procedure
Citations (8)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (25 uL), Heparin Plasma (25 uL)
  • Sensitivity
    3.8 pg/mL
  • Assay Range
    7.8 - 500 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse and rat PDGF-AB
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse/Rat PDGF-AB Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse/rat PDGF-AB in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat PDGF-AB and antibodies raised against recombinant rat PDGF. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse/rat PDGF-AB showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse/rat PDGF-AB.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 89 127 89
Mean 19 58 141 18 60 147
Standard Deviation 1.4 2.9 4.6 1.8 3.6 7.4
CV% 7.4 5 3.3 10 6 5

Recovery

The recovery of mouse/rat PDGF-AB spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Mouse Cell Culture Supernates (n=6) 96 86-113
Rat Cell Culture Supernates (n=5) 95 80-108
Rat EDTA Plasma (n=6) 104 80-119
Rat Heparin Plasma (n=6) 98 87-109
Rat Serum (n=6) 97 82-112
Linearity
To assess the linearity of the assay, samples spiked with or containing PDGF-AB in each matrix were diluted with Calibrator Diluent and then assayed.
 PDGF-AB [HRP]
 PDGF-AB [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PDGF-AB
The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).
  • Long Name:
    Platelet-derived Growth Factor AB
  • Alternate Names:
    PDGFAB; PDGF-AB
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Sample Type
  1. Adiponectin enhances bone marrow mesenchymal stem cell resistance to flow shear stress through AMP-activated protein kinase signaling
    Sci Rep, 2016;6(0):28752.
    Species: Rat
    Sample Type: Cell Culture Supernates
  2. Deficiency of liver sinusoidal scavenger receptors stabilin-1 and -2 in mice causes glomerulofibrotic nephropathy via impaired hepatic clearance of noxious blood factors.
    Authors: Schledzewski K, Geraud C, Arnold B, Wang S, Grone HJ, Kempf T, Wollert KC, Straub BK, Schirmacher P, Demory A, Schonhaber H, Gratchev A, Dietz L, Thierse HJ, Kzhyshkowska J, Goerdt S
    J. Clin. Invest., 2011;121(2):703-14.
    Species: Mouse
    Sample Type: Plasma
  3. Antifibrotic action of pirfenidone and prednisolone: different effects on pulmonary cytokines and growth factors in bleomycin-induced murine pulmonary fibrosis.
    Authors: Oku H, Shimizu T, Kawabata T, Nagira M, Hikita I, Ueyama A, Matsushima S, Torii M, Arimura A
    Eur. J. Pharmacol., 2008;590(1):400-8.
    Species: Mouse
    Sample Type: Tissue Homogenates
  4. Observations on the microvasculature of bone defects filled with biodegradable nanoparticulate hydroxyapatite.
    Authors: Kilian O, Wenisch S, Karnati S, Baumgart-Vogt E, Hild A, Fuhrmann R, Jonuleit T, Dingeldein E, Schnettler R, Franke RP
    Biomaterials, 2008;29(24):3429-37.
    Species: Porcine
    Sample Type: Plasma
  5. Inhibition of rho family functions by lovastatin promotes myelin repair in ameliorating experimental autoimmune encephalomyelitis.
    Authors: Paintlia AS, Paintlia MK, Singh AK, Singh I
    Mol. Pharmacol., 2008;73(5):1381-93.
    Species: Rat
    Sample Type: Serum
  6. The role of the receptor for advanced glycation end-products in lung fibrosis.
    Authors: He M, Kubo H, Ishizawa K, Hegab AE, Yamamoto Y, Yamamoto H, Yamaya M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(6):L1427-36.
    Species: Mouse
    Sample Type: BALF
  7. Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy.
    Authors: Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(43):17069-74.
    Species: Mouse
    Sample Type: Plasma
  8. A new murine model for bronchiolitis obliterans post-bone marrow transplant.
    Authors: Panoskaltsis-Mortari A, Tram KV, Price AP, Wendt CH, Blazar BR
    Am. J. Respir. Crit. Care Med., 2007;176(7):713-23.
    Species: Mouse
    Sample Type: Serum

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