Mouse/Rat Transglutaminase 2/TGM2 Antibody

Catalog # Availability Size / Price Qty
AF5418
AF5418-SP
Detection of Mouse and Rat Transglutaminase 2/TGM2 by Western Blot.
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Mouse/Rat Transglutaminase 2/TGM2 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat Transglutaminase 2/TGM2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) TGM2 and rhTGM7 is observed, and less than 1% cross‑reactivity with rhTGM3 and rhTGM4 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Transglutaminase 2/TGM2
Ala2-Ala686
Accession # P21981
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse and Rat Transglutaminase 2/TGM2 by Western Blot. View Larger

Detection of Mouse and Rat Transglutaminase 2/
TGM2 by Western Blot.

Western blot shows lysates of mouse lung tissue and rat lung tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat Transglutaminase 2/TGM2 Polyclonal Antibody (Catalog # AF5418) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Transglutaminase 2/TGM2 at approximately 80-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Transglutaminase 2/TGM2

Transglutaminase 2 (TG2), encoded by the Tgm2 gene, is also known as tissue Transglutaminase (tTG), Transglutaminase C (TGC), and protein-glutamine-gamma -glutamyltransferase. It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1‑3). In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP (4) and has intrinsic kinase activity (5). TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases (6, 7).

References
  1. Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
  2. Chen, J.S.K. and Mehta K. (1999) Internat. J. Biochem. Cell Biol. 31:817.
  3. Griffin, M. et al. (2002) Biochem. J. 368:377.
  4. Lai, T.S. et al. (1998) J. Biol. Chem. 273:1776.
  5. Mishra, S. et al. (2007) J. Biol. Chem. 282:18108.
  6. Kim, S-Y. et al. (2002) Neurochem. Int. 40:85.
  7. Lesort, M. et al. (2000) Prog. Neurobiol. 61:439.
Entrez Gene IDs
7052 (Human); 21817 (Mouse); 56083 (Rat)
Alternate Names
C polypeptide; EC 2.3.2.13; G-ALPHA-h; protein-glutamine gamma-glutamyltransferase 2; protein-glutamine-gamma-glutamyltransferase; TG(C); TG2; TGase C; TGase H; TGase-2; TGase-H; TGC; TGCGNAH; TGM2; Tissue transglutaminase; transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase); Transglutaminase 2; Transglutaminase C; Transglutaminase H; transglutaminase-2; tTG

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