Detects mouse and rat Transglutaminase 2/TGM2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) TGM2 and rhTGM7 is observed, and less than 1% cross‑reactivity with rhTGM3 and rhTGM4 is observed.
Polyclonal Sheep IgG
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Transglutaminase 2/TGM2 Ala2-Ala686 Accession # P21981
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse and Rat Transglutaminase 2/ TGM2 by Western Blot.
Western blot shows lysates of mouse lung tissue and rat lung tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat Transglutaminase 2/TGM2 Polyclonal Antibody (Catalog # AF5418) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Transglutaminase 2/TGM2 at approximately 80-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Transglutaminase 2/TGM2
Transglutaminase 2 (TG2), encoded by the Tgm2 gene, is also known as tissue Transglutaminase (tTG), Transglutaminase C (TGC), and protein-glutamine-gamma -glutamyltransferase. It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1‑3). In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP (4) and has intrinsic kinase activity (5). TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases (6, 7).
Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
Chen, J.S.K. and Mehta K. (1999) Internat. J. Biochem. Cell Biol. 31:817.
Griffin, M. et al. (2002) Biochem. J. 368:377.
Lai, T.S. et al. (1998) J. Biol. Chem. 273:1776.
Mishra, S. et al. (2007) J. Biol. Chem. 282:18108.
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