Mouse Resistin Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1069
Product Details
Citations (1)
FAQs
Supplemental Products
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Mouse Resistin Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Resistin in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) Resistin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Resistin (R&D Systems, Catalog # 1069-RN)
Ser21-Ser114
Accession # Q99P87
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Resistin (Catalog # 1069-RN)

Mouse Resistin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse Resistin Antibody (Catalog # MAB1069)

Standard: Recombinant Mouse Resistin Protein (Catalog # 1069-RN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Resistin

Resistin, also known as adipocyte-specific secretory factor (ADSF) and found in inflammatory zone 3 (FIZZ3), is a member of a family of secreted cysteine-rich peptide hormones that also includes Resistin-like molecules RELM alpha, beta, and gamma. These molecules play important roles in inflammation, glucose metabolism, and insulin resistance (1, 2). Mature mouse Resistin is a 12 kDa protein with an N-terminal alpha -helical domain and a C-terminal beta -sandwich domain that is stabilized by multiple intrachain disulfide bonds (3). Resistin circulates as noncovalent trimers and disulfide-linked hexamers, with the trimeric form showing greater bioactivity (3, 4). Resistin can also form multimers with RELM beta  (4). Mature mouse Resistin shares 56% and 72% amino acid (aa) sequence identity with human and rat Resistin, respectively. It shares 34% - 42% aa sequence identity with mouse RELM alpha, beta, and gamma. In rodents, Resistin is expressed by adipocytes and in the pituitary and arcuate nucleus of the hypothalamus (5 - 9). It is upregulated during adipogenesis, in obesity, and by insulin or a high carbohydrate diet (5, 6). This is in contrast to human Resistin which is produced by macrophages and monocytes but not by adipocytes (9 - 12). Mouse Resistin induces proinflammatory molecule production in adipocytes and promotes hepatic gluconeogenesis and insulin resistance (5, 7). Human Resistin promotes lipolysis by human and mouse adipocytes, but mouse Resistin does not promote lipolysis by adipocytes of either species (10). Both mouse and human Resistin promote vascular endothelial cell sprouting in vitro and inflammatory reactions in vivo (13 - 16).

References
  1. Adeghate, E. (2004) Cell. Mol. Life Sci. 61:2485.
  2. Asano, T. et al. (2006) Curr. Diabetes Rev. 2:449.
  3. Patel, S.D. et al. (2004) Science 304:1154.
  4. Chen, J. et al. (2002) J. Endocrinol. 175:499.
  5. Steppan, C.M. et al. (2001) Nature 409:307.
  6. Kim, K-H. et al. (2001) J. Biol. Chem. 276:11252.
  7. Banerjee, R.R. et al. (2004) Science 303:1195.
  8. Rajala, M.W. et al. (2002) Mol. Endocrinol. 16:1920.
  9. Morash, B.A. et al. (2002) FEBS Lett. 526:26.
  10. Ort, T. et al. (2005) Endocrinology 146:2200.
  11. Nagaev, I. et al. (2006) Plos ONE 1:e31.
  12. Savage, D.B. et al. (2001) Diabetes 50:2199.
  13. Mu, H. et al. (2006) Cardiovasc. Res. 70:146.
  14. Robertson, S.A. et al. (2008) Regul. Pept. August 5 epub.
  15. Bokarewa, M. et al. (2005) J. Immunol. 174:5789.
  16. Burnett, M.S. et al. (2005) Atherosclerosis 182:241.
Entrez Gene IDs
56729 (Human); 57264 (Mouse)
Alternate Names
Adipose tissue-specific secretory factor; ADSF; ADSFMGC126609; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; FIZZ3; FIZZ3MGC126603; found in inflammatory zone 3; HXCP1; Resistin; RETN; RETN1; RSTNXCP1

Product Datasheets

Citation for Mouse Resistin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Facilitated in vivo synthesis of ribonucleic acid and protein via T7 RNA polymerase.
    Authors: Wang TH, Yu SH, Au LC
    Anal. Biochem., 2008;375(1):97-104.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Slot Blot

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