Mouse Resistin Quantikine ELISA Kit

(14 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    8 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse Resistin
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse Resistin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Resistin in cell culture supernates, mouse serum, and plasma. It contains E. coli-expressed recombinant mouse Resistin and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse Resistin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse Resistin.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation3.


The recovery of mouse Resistin spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=8) 99 85-108
EDTA Plasma (n=8) 97 87-107
Heparin Plasma (n=8) 102 89-113
Serum (n=6) 99 93-108
To assess the linearity of the assay, samples containing high concentrations of mouse Resistin in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse Resistin Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Resistin
Resistin, also known ADSF and FIZZ3, is a secreted peptide hormone that circulates as either a noncovalent trimer, or a disulfide-linked homohexamer. Resistin is expressed by white adipocytes, splenocytes, astrocytes, and anterior pituitary epithelium. It blocks Insulin-stimulated uptake of glucose by adipocytes and promotes glucose release by hepatocytes. High circulating Insulin levels induce Resistin secretion which then antagonize Insulin action.
    • Entrez Gene IDs
      56729 (Human); 57264 (Mouse);
    • Alternate Names
      Adipose tissue-specific secretory factor; ADSF; ADSFMGC126609; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; FIZZ3; FIZZ3MGC126603; found in inflammatory zone 3; HXCP1; Resistin; RETN; RETN1; RSTNXCP1;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    14 Citations: Showing 1 - 10
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    Sample Type
    1. Leptin Production by Encapsulated Adipocytes Increases Brown Fat, Decreases Resistin, and Improves Glucose Intolerance in Obese Mice
      Authors: DJ DiSilvestr, E Melgar-Ber, R Yasmeen, P Fadda, LJ Lee, A Kalyanasun, CL Gilor, O Ziouzenkov
      PLoS ONE, 2016;11(4):e0153198.
      Species: Mouse
      Sample Type: Plasma
    2. Induction of adipocyte hyperplasia in subcutaneous fat depot alleviated type 2 diabetes symptoms in obese mice.
      Authors: Lu Q, Li M, Zou Y, Cao T
      Obesity (Silver Spring), 2014;22(7):1623-31.
      Species: Mouse
      Sample Type: Serum
    3. PKClambda haploinsufficiency prevents diabetes by a mechanism involving alterations in hepatic enzymes.
      Authors: Sajan M, Ivey R, Lee M, Mastorides S, Jurczak M, Samuels V, Shulman G, Braun U, Leitges M, Farese R
      Mol Endocrinol, 2014;28(7):1097-107.
      Species: Mouse
      Sample Type: Serum
    4. sigma1B adaptin regulates adipogenesis by mediating the sorting of sortilin in adipose tissue.
      Authors: Baltes J, Larsen J, Radhakrishnan K, Geumann C, Kratzke M, Petersen C, Schu P
      J Cell Sci, 2014;127(0):3477-87.
      Species: Mouse
      Sample Type: Serum
    5. Ox-LDL induces ER stress and promotes the adipokines secretion in 3T3-L1 adipocytes.
      Authors: Chen Y, Chen M, Wu Z, Zhao S
      PLoS ONE, 2013;8(10):e81379.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    6. HCV core-induced nonobese hepatic steatosis is associated with hypoadiponectinemia and is ameliorated by adiponectin administration.
      Authors: Chang ML, Yeh HC, Tsou YK
      Obesity (Silver Spring), 2012;20(7):1474-80.
      Species: Mouse
      Sample Type: Serum
    7. Tangeretin stimulates glucose uptake via regulation of AMPK signaling pathways in C2C12 myotubes and improves glucose tolerance in high-fat diet-induced obese mice.
      Mol. Cell. Endocrinol., 2012;358(1):127-34.
      Species: Mouse
      Sample Type: Serum
    8. Castration-induced changes in mouse epididymal white adipose tissue.
      Authors: Floryk D, Kurosaka S, Tanimoto R, Yang G, Goltsov A, Park S, Thompson TC
      Mol. Cell. Endocrinol., 2011;345(1):58-67.
      Species: Mouse
      Sample Type: Serum
    9. eNOS plays a major role in adiponectin synthesis in adipocytes.
      Authors: Koh EH, Kim M, Ranjan KC, Kim HS, Park HS, Oh KS, Park IS, Lee WJ, Kim MS, Park JY, Youn JH, Lee KU
      Am. J. Physiol. Endocrinol. Metab., 2010;298(4):E846-53.
      Species: Mouse
      Sample Type: Plasma
    10. PPARgamma in the endothelium regulates metabolic responses to high-fat diet in mice.
      Authors: Kanda T, Brown JD, Orasanu G, Vogel S, Gonzalez FJ, Sartoretto J, Michel T, Plutzky J
      J. Clin. Invest., 2009;119(1):110-24.
      Species: Mouse
      Sample Type: Serum
    11. Regulation of the brown and white fat gene programs through a PRDM16/CtBP transcriptional complex.
      Authors: Kajimura S, Seale P, Tomaru T, Erdjument-Bromage H, Cooper MP, Ruas JL, Chin S, Tempst P, Lazar MA, Spiegelman BM
      Genes Dev., 2008;22(10):1397-409.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    12. Octylphenol stimulates resistin gene expression in 3T3-L1 adipocytes via the estrogen receptor and extracellular signal-regulated kinase pathways.
      Authors: Lee MJ, Lin H, Liu CW, Wu MH, Liao WJ, Chang HH, Ku HC, Chien YS, Ding WH, Kao YH
      Am. J. Physiol., Cell Physiol., 2008;294(6):C1542-51.
      Species: Mouse
      Sample Type: Serum
    13. Role and regulation of adipokines during zymosan-induced peritoneal inflammation in mice.
      Authors: Pini M, Gove ME, Sennello JA, van Baal JW, Chan L, Fantuzzi G
      Endocrinology, 2008;149(8):4080-5.
      Species: Mouse
      Sample Type: Serum
    14. Neuropeptide Y acts directly in the periphery on fat tissue and mediates stress-induced obesity and metabolic syndrome.
      Authors: Kuo LE, Kitlinska JB, Tilan JU, Li L, Baker SB, Johnson MD, Lee EW, Burnett MS, Fricke ST, Kvetnansky R, Herzog H, Zukowska Z
      Nat. Med., 2007;13(7):803-11.
      Species: Mouse
      Sample Type: Plasma
    Expand to show all 14 Citations
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