Mouse S100A16 Antibody

Catalog # Availability Size / Price Qty
AF6397
AF6397-SP
Mouse S100A16 Antibody in Data
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Mouse S100A16 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse S100A16 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human S100A16 is observed, and less than 1% cross-reactivity with recombinant mouse S100A4, recombinant human (rh) S100A2, and rhS100A10 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E.coli-derived recombinant mouse S100A16
Ala2-Ser124
Accession # NP_080692
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse S100A16 antibody by Western Blot. View Larger

Detection of Mouse S100A16 by Western Blot. Western blot shows lysates of mouse brain (cerebellum) tissue. PVDF Membrane was probed with 1 µg/mL of Mouse S100A16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6397) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A16 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A16

Mouse S100A16 (S100 calcium-binding protein A16; also S100F and AAG13) is a 15-18 kDa member of the S100 family of calcium-binding proteins. It is found in both cytoplasm (when Ca is high) and nucleus (when Ca is low), and its expression appears to be restricted to astrocytes found in select areas of the brain. S100A16 is 124 amino acids (aa) in length. Based on human, it will contain an N-terminal non-Ca-binding EF-hand domain (aa 12-47), a C-terminal Ca-binding EF-hand domain (aa 54-89), and a putative FGF-1 binding site (aa 91-94). There is one potential myristoylation site (aa 54-59) and a phosphorylation site at Ser124. S100A16 will homodimerize, and perhaps form higher order oligomers. Full length mouse S100A16 shares 95% aa identity with rat S100A16.  Relative to human S100A16, mouse S100A16 possesses a 21 aa Glu/Gln-rich insertion after Glu98 that reduces the shared aa homology between mouse and human S100A16 to 72% aa identity. 

Long Name
S100 Calcium Binding Protein A16
Entrez Gene IDs
140576 (Human); 67860 (Mouse); 361991 (Rat)
Alternate Names
AAG13; Aging-associated gene 13 protein; aging-associated protein 13; DT1P1A7; MGC17528; protein S100-A16; Protein S100-F; S100 calcium binding protein A16; S100A16; S100F; S100FS100 calcium-binding protein A16

Product Datasheets

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