Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all S100A16 Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant mouse S100A16
Ala2-Ser124
Accession # NP_080692

Specificity

Detects mouse S100A16 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human S100A16 is observed, and less than 1% cross-reactivity with recombinant mouse S100A4, recombinant human (rh) S100A2, and rhS100A10 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse S100A16 Antibody

Detection of Mouse S100A16 antibody by Western Blot.

Detection of Mouse S100A16 by Western Blot.

Western blot shows lysates of mouse brain (cerebellum) tissue. PVDF Membrane was probed with 1 µg/mL of Mouse S100A16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6397) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A16 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse S100A16 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse brain (cerebellum) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100A16

Mouse S100A16 (S100 calcium-binding protein A16; also S100F and AAG13) is a 15-18 kDa member of the S100 family of calcium-binding proteins. It is found in both cytoplasm (when Ca is high) and nucleus (when Ca is low), and its expression appears to be restricted to astrocytes found in select areas of the brain. S100A16 is 124 amino acids (aa) in length. Based on human, it will contain an N-terminal non-Ca-binding EF-hand domain (aa 12-47), a C-terminal Ca-binding EF-hand domain (aa 54-89), and a putative FGF-1 binding site (aa 91-94). There is one potential myristoylation site (aa 54-59) and a phosphorylation site at Ser124. S100A16 will homodimerize, and perhaps form higher order oligomers. Full length mouse S100A16 shares 95% aa identity with rat S100A16.  Relative to human S100A16, mouse S100A16 possesses a 21 aa Glu/Gln-rich insertion after Glu98 that reduces the shared aa homology between mouse and human S100A16 to 72% aa identity. 

Long Name

S100 Calcium Binding Protein A16

Alternate Names

AAG13, DT1P1A7, S100F

Entrez Gene IDs

140576 (Human); 67860 (Mouse); 361991 (Rat)

Gene Symbol

S100A16

UniProt

NP_080692

Additional S100A16 Products

Product Documents for Mouse S100A16 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse S100A16 Antibody

For research use only

Citations for Mouse S100A16 Antibody

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