Detects mouse S100A16 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human S100A16 is observed, and less than 1% cross-reactivity with recombinant mouse S100A4, recombinant human (rh) S100A2, and rhS100A10 is observed.
Detection of Mouse S100A16 by Western Blot.
Western blot shows lysates of mouse brain (cerebellum) tissue. PVDF Membrane was probed with 1 µg/mL of Mouse S100A16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6397) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A16 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse S100A16 (S100 calcium-binding protein A16; also S100F
and AAG13) is a 15-18 kDa member of the S100 family of calcium-binding
proteins. It is found in both cytoplasm (when
Ca is high) and nucleus (when Ca is low), and its expression appears to be restricted
to astrocytes found in select areas of the brain. S100A16 is 124 amino acids (aa) in length. Based on human,
it will contain an N-terminal non-Ca-binding EF-hand domain (aa 12-47), a
C-terminal Ca-binding EF-hand domain (aa 54-89), and a putative FGF-1 binding
site (aa 91-94). There is one potential
myristoylation site (aa 54-59) and a phosphorylation site at Ser124. S100A16 will homodimerize, and perhaps form
higher order oligomers. Full length mouse
S100A16 shares 95% aa identity with rat S100A16. Relative to human S100A16, mouse S100A16
possesses a 21 aa Glu/Gln-rich insertion after Glu98 that reduces the shared aa
homology between mouse and human S100A16 to 72% aa identity.
S100 Calcium Binding Protein A16
Entrez Gene IDs:
140576 (Human); 67860 (Mouse); 361991 (Rat)
AAG13; Aging-associated gene 13 protein; aging-associated protein 13; DT1P1A7; MGC17528; protein S100-A16; Protein S100-F; S100 calcium binding protein A16; S100A16; S100F; S100FS100 calcium-binding protein A16
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