Mouse S100A16 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse S100A16 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human S100A16 is observed, and less than 1% cross-reactivity with recombinant mouse S100A4, recombinant human (rh) S100A2, and rhS100A10 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E.coli-derived recombinant mouse S100A16
    Ala2-Ser124
    Accession # NP_080692
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse S100A16 by Western Blot. Western blot shows lysates of mouse brain (cerebellum) tissue. PVDF Membrane was probed with 1 µg/mL of Mouse S100A16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6397) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A16 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A16

Mouse S100A16 (S100 calcium-binding protein A16; also S100F and AAG13) is a 15-18 kDa member of the S100 family of calcium-binding proteins. It is found in both cytoplasm (when Ca is high) and nucleus (when Ca is low), and its expression appears to be restricted to astrocytes found in select areas of the brain. S100A16 is 124 amino acids (aa) in length. Based on human, it will contain an N-terminal non-Ca-binding EF-hand domain (aa 12-47), a C-terminal Ca-binding EF-hand domain (aa 54-89), and a putative FGF-1 binding site (aa 91-94). There is one potential myristoylation site (aa 54-59) and a phosphorylation site at Ser124. S100A16 will homodimerize, and perhaps form higher order oligomers. Full length mouse S100A16 shares 95% aa identity with rat S100A16.  Relative to human S100A16, mouse S100A16 possesses a 21 aa Glu/Gln-rich insertion after Glu98 that reduces the shared aa homology between mouse and human S100A16 to 72% aa identity. 

  • Long Name:
    S100 Calcium Binding Protein A16
  • Entrez Gene IDs:
    140576 (Human); 67860 (Mouse); 361991 (Rat)
  • Alternate Names:
    AAG13; Aging-associated gene 13 protein; aging-associated protein 13; DT1P1A7; MGC17528; protein S100-A16; Protein S100-F; S100 calcium binding protein A16; S100A16; S100F; S100FS100 calcium-binding protein A16
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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