Mouse S100A4 Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse S100A4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse S100A6, 8, 9, 10, 13, 16, recombinant human (rh) S100A1, 2, 7, 11, or rhS100P is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 438709
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse S100A4
    Ala2-Lys101
    Accession # P07091
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse S100A4 by Western Blot. Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose‑like cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse S100A4 Monoclonal Antibody (Catalog # MAB4138) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for S100A4 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A4

S100A4 (also named Metastasin, Mtsl and Calvasculin) is an 11 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of proteins (1‑5). S100 family members belong to the EF-hand superfamily of Ca++-binding proteins. These participate in both calcium‑dependent and calcium‑independent protein‑protein interactions. The hallmark of this superfamily is the EF-hand motif that consists of a Ca++-binding site flanked by two alpha -helices (helix E and helix F) that were originally identified in a right-handed model of carp muscle calcium‑binding protein (6). Mouse S100A4 is 101 amino acids (aa) in length (1, 2). It contains two EF hand domains (aa 12‑47 and aa 50‑85). The first domain has a 14 aa cation-binding motif and binds Ca++ with low affinity. The second Ca++-binding motif is 12 aa in length and binds Ca++ with high affinity. S100A4 has no classical signal sequence but is secreted from cells (3, 7). Mouse S100A4 shares 93%, 96% and 89% aa identity with human, rat and canine S100A4, respectively. S100A4 exists as dimer (8, 9, 10). Extracellular S100A4 is reported to induce MMP production, activate MMPs, promote neurite outgrowth and stimulate cardiomyocyte proliferation (4, 10, 11, 12, 13). Within the cell, dimers are likely the functional unit. Here, they are constitutive homo‑ or heterodimers (with S100A1) that interact with Ca++, undergo a conformational change, and subsequently bind to cytoplasmic targets. Known targets include p53, Myosin heavy chain II, F-actin and Liprin beta 1 (4, 14). In general, it can be said that S100A4 blocks target phosphorylation and multimerization (4, 7, 14). S100A4 activity has been associated with cell transformation. It seems likely this is either coincidental, or a consequence, rather than a cause of transformation (3).

  • References:
    1. Jackson-Grusby, L.L. et al. (1987) Nucleic Acids Res. 15:6677.
    2. Goto, K. et al. (1988) J. Biochem. 103:48.
    3. Garrett, S.C. et al. (2006) J. Biol. Chem. 281:677.
    4. Santamaria-Kisiel, L. et al. (2006) Biochem. J. 396:201.
    5. Donato, R. (2001) Int. J. Biochem. Mol. Biol. 33:637.
    6. Kretsinger, R.H. and C.E. Nockolds (1973) J. Biol. Chem. 248:3313.
    7. Helfman, D.M. et al. (2005) Br. J. Cancer 92:1955.
    8. Burkitt, W.I. et al. (2003) Biochem. Soc. Trans. 31:985.
    9. Vallaly, K.M. et al. (2002) Biochemistry 41:12670.
    10. Novitskaya, V. et al. (2000) J. Biol. Chem. 275:41278.
    11. Stary, M. et al. (2006) Biochem. Biophys. Res. Commun. 343:555.
    12. Semov, A. et al. (2005) J. Biol. Chem. 280:20833.
    13. Saleem, M. et al. (2006) Proc. Natl. Acad. Sci. USA 103:14825.
    14. Kriajevska, M. et al. (2002) J. Biol. Chem. 277:5229.
  • Long Name:
    S100 Calcium Binding Protein A4
  • Entrez Gene IDs:
    6275 (Human); 20198 (Mouse); 24615 (Rat)
  • Alternate Names:
    18A2; 42A; Calvasculin; CAPL; CAPLS100 calcium binding protein A4 (calcium protein, calvasculin, metastasin; fibroblast-specific protein-1,42A; FSP1; leukemia multidrug resistance associated protein; malignant transformation suppression 1; Metastasin; MTS1; murine placental homolog); P9KA; PEL98; Placental calcium-binding protein; Protein Mts1; protein S100-A4; S100 calcium binding protein A4; S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog); S100 calcium-binding protein A4; S100A4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Functional expression, characterization and application of the human S100A4 protein.
    Authors: Wang, Degang, Zhang, Jianwei, Liu, Ziquan, Chen, Yunyun, Xu, Chuanxia, Zhang, Zhiqing, Liu, Xiaohua, Wu, Lei, Zhou, Xuesi, Meng, Xiangyan, Li, Hua, Liu, Hongtao, Jiang, Zifeng, Wang, Tianhui
    Mol Med Rep, 2015;11(1):175-81.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Isotype Controls
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Rat IgG2B Isotype Control

Ctrl MAB0061 27  
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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG2B Isotype Control

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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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