S100A8 in NMuMG Mouse Cell Line. S100A8 was detected in immersion fixed NMuMG mouse mammary gland epithelial cell line using 10 µg/mL Goat Anti-Mouse S100A8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3059) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse S100A8, also known as CP-10, Calgranulin A and MRP8, is a 10 kDa
member of the S100 family of calcium-binding proteins. S100A8 contains
short sequential modules, including an N-terminal alpha -helix, a Ca++-binding
EF-hand segment, a short central linker region, a second EF-hand
segment, and a C-terminal alpha -helix. S100A8 noncovalently heterodimerizes
with S100A9. In the presence of Ca++, the heterodimers form
heterotetramers. The dimeric complex is found both intracellularly and
extracellularly. It binds to heparan sulfate and is chemotactic for
PMNs and macrophages. The amino acid sequence of mouse S100A8 is 80%
and 57% identical to that of rat and human S100A8, respectively.
S100 Calcium Binding Protein A8
Entrez Gene IDs:
6279 (Human); 20201 (Mouse)
60B8AG; CAGACP-10; Calgranulin A; calgranulin-A; Calprotectin L1L subunit; CFAG; CFAGL1Ag; CGLA; Cystic fibrosis antigen; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP8; MRP-8; MRP8S100 calcium binding protein A8 (calgranulin A); NIF; P8; protein S100-A8; S100 calcium binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100A8; Urinary stone protein band A
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