Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Flow Cytometry, ELISA Development, IHC-F

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all S100A8 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse S100A8
Pro2-Glu89
Accession # P27005

Specificity

Detects mouse S100A8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) S100A9, rmS100A10, and recombinant human S100B is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse S100A8 Antibody

Detection of Mouse S100A8 antibody by Western Blot.

Detection of Mouse S100A8 by Western Blot.

Western blot shows lysates of mouse spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for S100A8 at approximately 10-11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

S100A8 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

S100A8 in Mouse Splenocytes.

S100A8 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

S100A8 antibody in NMuMG Mouse Cell Line by Immunocytochemistry (ICC).

S100A8 in NMuMG Mouse Cell Line.

S100A8 was detected in immersion fixed NMuMG mouse mammary gland epithelial cell line using 10 µg/mL Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Mouse S100A8 antibody by Simple WesternTM.

Detection of Mouse S100A8 by Simple WesternTM.

Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A8 at approximately 9 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of S100A8 by Western Blot

Detection of S100A8 by Western Blot

S100A8/A9 proteins augment differentiation and osteoclastic activity derived from iOCPs but not from hOCPs. a Expression of S100A8, S100A9 and RAGE in sorted control and inflamed BM iOCPs and hOCPs. The protein phosphatase 2A catalytic subunit (PP2Ac) is shown as a loading control. b Densitometry measurements of three biological repeats (no statistical analysis presented). c Sorted control and inflamed BM iOCPs and hOCPs (5 × 104) were cultured on the Osteo assay surface with or without anti-RAGE blocking antibodies (bar: 50 µm). d Pit area quantitation. e Sorted iOCPs and hOCPs (5 × 104) from the BM of control mice were cultured on the Osteo assay surface in combination with a recombinant S100A8/A9 heterodimer and anti-RAGE blocking antibodies as indicated (bar: 50 µm). f Pit area quantitation. c–f Depict representative results for two independent experiments, n = 5 for each group. Line: median, box: 25th-75th percentile, whiskers: range. *P < 0.05 (Mann–Whitney test and Holm multiplicity correction) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35396510), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse S100A8 by Immunohistochemistry

Detection of Mouse S100A8 by Immunohistochemistry

iASPP deficient keratinocytes induce pro-inflammatory gene expression in vitro and attract macrophages in vivo(A) qPCR analysis of mRNA expression levels of inflammatory genes (and iASPP gene Ppp1r13l) in iASPP WT and KO primary keratinocytes at 0, 1, and 6 h after treatment with TNF-alpha. Values are mean + SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; n = 3 biological replicates.(B) Left, IHC staining of S100a8 and S100a9 in untreated skin sections from iASPP WT and KO mice (adjacent sections). Scale bar, 50 μm. Right, IB of iASPP and S100a9 expression levels in iASPP WT (−tamoxifen) and KO (+tamoxifen) primary keratinocytes.(C) H&E analysis of acetone- or TPA-treated skin sections from iASPP WT and KO mice. Scale bar, 50 μm. Histograms below show epidermal thickness in the same samples. Values are mean ± SD. n = 3 (WT) and n = 4 (KO) mice in acetone cohort; n = 4 (WT), n = 3 (KO) mice in TPA cohort.(D) IHC staining of F4/80-positive macrophages in acetone- or TPA-treated skin sections from iASPP WT and KO mice. Scale bar, 50 μm. Histograms below show quantification of the same samples. Values are mean + SD. Same cohort as in (C).See STAR Methods for p calculations for (A), (C), and (D). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36261000), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse S100A8 by Immunohistochemistry

Detection of Mouse S100A8 by Immunohistochemistry

iASPP deficient keratinocytes induce pro-inflammatory gene expression in vitro and attract macrophages in vivo(A) qPCR analysis of mRNA expression levels of inflammatory genes (and iASPP gene Ppp1r13l) in iASPP WT and KO primary keratinocytes at 0, 1, and 6 h after treatment with TNF-alpha. Values are mean + SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; n = 3 biological replicates.(B) Left, IHC staining of S100a8 and S100a9 in untreated skin sections from iASPP WT and KO mice (adjacent sections). Scale bar, 50 μm. Right, IB of iASPP and S100a9 expression levels in iASPP WT (−tamoxifen) and KO (+tamoxifen) primary keratinocytes.(C) H&E analysis of acetone- or TPA-treated skin sections from iASPP WT and KO mice. Scale bar, 50 μm. Histograms below show epidermal thickness in the same samples. Values are mean ± SD. n = 3 (WT) and n = 4 (KO) mice in acetone cohort; n = 4 (WT), n = 3 (KO) mice in TPA cohort.(D) IHC staining of F4/80-positive macrophages in acetone- or TPA-treated skin sections from iASPP WT and KO mice. Scale bar, 50 μm. Histograms below show quantification of the same samples. Values are mean + SD. Same cohort as in (C).See STAR Methods for p calculations for (A), (C), and (D). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36261000), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse S100A8 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed NMuMG mouse mammary gland epithelial cell line and immersion fixed mouse splenocytes

Simple Western

2.5 µg/mL
Sample: Mouse spleen tissue

Western Blot

0.25 µg/mL
Sample: Mouse spleen tissue

Reviewed Applications

Read 2 reviews rated 3.5 using AF3059 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100A8

Mouse S100A8, also known as CP-10, Calgranulin A and MRP8, is a 10 kDa member of the S100 family of calcium-binding proteins. S100A8 contains short sequential modules, including an N-terminal alpha -helix, a Ca++-binding EF-hand segment, a short central linker region, a second EF-hand segment, and a C-terminal alpha -helix. S100A8 noncovalently heterodimerizes with S100A9. In the presence of Ca++, the heterodimers form heterotetramers. The dimeric complex is found both intracellularly and extracellularly. It binds to heparan sulfate and is chemotactic for PMNs and macrophages. The amino acid sequence of mouse S100A8 is 80% and 57% identical to that of rat and human S100A8, respectively.

Long Name

S100 Calcium Binding Protein A8

Alternate Names

Calgranulin A, CFAG, MRP8

Entrez Gene IDs

6279 (Human); 20201 (Mouse)

Gene Symbol

S100A8

UniProt

P27005

Additional S100A8 Products

Product Documents for Mouse S100A8 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse S100A8 Antibody

For research use only

Citations for Mouse S100A8 Antibody

Customer Reviews for Mouse S100A8 Antibody (2)

3.5 out of 5
2 Customer Ratings
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 20098622
    Species: Mouse
    Verified Customer | Posted 01/07/2015
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23548910
    Species: Mouse
    Verified Customer | Posted 01/07/2015

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