Mouse SCF Quantikine ELISA Kit

(13 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL)
  • Sensitivity
    5 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant mouse SCF
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse SCF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse SCF levels in cell culture supernates, serum, and EDTA plasma. It contains E. coli-expressed recombinant mouse SCF and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse SCF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse SCF.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation2.5630.55.38.947.4


The recovery of mouse SCF spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 105 99-113
EDTA Plasma (n=4) 102 84-113
Serum (n=5) 101 92-115
To assess the linearity of the assay, five or more samples spiked with various concentrations of mouse SCF in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse SCF Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: SCF/c-kit Ligand
Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. The ECD shows both N-linked and O-linked glycosylation. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice. An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Within the ECD of the short isoform (corresponding to this recombinant protein), human SCF shares 75 - 83% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF-R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.
    • Long Name
      Stem Cell Factor
    • Entrez Gene IDs
      4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline);
    • Alternate Names
      c-kit Ligand; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCFStem cell factor; SFc-Kit ligand; SLF; steel factor;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 13
    Filter your results:

    Sample Type
    1. JAM-B regulates maintenance of hematopoietic stem cells in the bone marrow.
      Authors: Arcangeli ML, Frontera V, Bardin F, Obrados E, Adams S, Chabannon C, Schiff C, Mancini SJ, Adams RH, Aurrand-Lions M
      Blood, 2011;118(17):4609-19.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    2. Rosuvastatin reduces atherosclerotic lesions and promotes progenitor cell mobilisation and recruitment in apolipoprotein E knockout mice.
      Authors: Schroeter MR, Humboldt T, Schafer K, Konstantinides S
      Atherosclerosis, 2009;205(1):63-73.
      Species: Mouse
      Sample Type: Plasma
    3. Ectodomain shedding of FLT3 ligand is mediated by TNF-alpha converting enzyme.
      Authors: Horiuchi K, Morioka H, Takaishi H, Akiyama H, Blobel CP, Toyama Y
      J. Immunol., 2009;182(12):7408-14.
      Species: Mouse
      Sample Type: Serum
    4. Cell-nonautonomous function of Id1 in the hematopoietic progenitor cell niche.
      Authors: Suh HC, Ji M, Gooya J, Lee M, Klarmann KD, Keller JR
      Blood, 2009;114(6):1186-95.
      Species: Mouse
      Sample Type: Serum
    5. Direct injection of kit ligand-2 lentivirus improves cardiac repair and rescues mice post-myocardial infarction.
      Authors: Higuchi K, Ayach B, Sato T, Chen M, Devine SP, Rasaiah VI, Dawood F, Yanagisawa T, Tei C, Takenaka T, Liu PP, Medin JA
      Mol. Ther., 2009;17(2):262-8.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    6. The lineage-c-Kit+Sca-1+ cell response to Escherichia coli bacteremia in Balb/c mice.
      Authors: Zhang P, Nelson S, Bagby GJ, Siggins R, Shellito JE, Welsh DA
      Stem Cells, 2008;26(7):1778-86.
      Species: Mouse
      Sample Type: Plasma
    7. SCF-mediated mast cell infiltration and activation exacerbate the inflammation and immunosuppression in tumor microenvironment.
      Authors: Huang B, Lei Z, Zhang GM, Li D, Song C, Li B, Liu Y, Yuan Y, Unkeless J, Xiong H, Feng ZH
      Blood, 2008;112(4):1269-79.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    8. Reversion of immune tolerance in advanced malignancy: modulation of myeloid-derived suppressor cell development by blockade of stem-cell factor function.
      Authors: Pan PY, Wang GX, Yin B, Ozao J, Ku T, Divino CM, Chen SH
      Blood, 2007;111(1):219-28.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    9. Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy.
      Authors: Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS
      Proc. Natl. Acad. Sci. U.S.A., 2007;104(43):17069-74.
      Species: Mouse
      Sample Type: Plasma
    10. Enalapril increases ischemia-induced endothelial progenitor cell mobilization through manipulation of the CD26 system.
      Authors: Wang CH, Verma S, Hsieh IC, Chen YJ, Kuo LT, Yang NI, Wang SY, Wu MY, Hsu CM, Cheng CW, Cherng WJ
      J. Mol. Cell. Cardiol., 2006;41(1):34-43.
      Species: Mouse
      Sample Type: Plasma
    11. A novel polysaccharide of black soybean promotes myelopoiesis and reconstitutes bone marrow after 5-flurouracil- and irradiation-induced myelosuppression.
      Authors: Liao HF, Chen YJ, Yang YC
      Life Sci., 2005;77(4):400-13.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    12. Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand.
      Authors: Heissig B, Hattori K, Dias S, Friedrich M, Ferris B, Hackett NR, Crystal RG, Besmer P, Lyden D, Moore MA, Werb Z, Rafii S
      Cell, 2002;109(5):625-37.
      Species: Mouse
      Sample Type: Plasma
    13. A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
      Authors: Shih CC, Hu MC, Hu J
      Blood, 2000;95(6):1957-66.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    Expand to show all
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine Wash Buffer 1

    ELISA WA126 5
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....

    Have you used Mouse SCF Quantikine ELISA Kit?

    Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Order Details

    Contact Information

    Certificate of Analysis Lookup

    The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

    Certificate of Analysis Lookup

    Research Areas of Interest
    R&D Systems Guarantee

    Customers Who Viewed
    This Item Also Viewed


    Customer Information