Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 300812
View all SDNSF/MCFD2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SDNSF/MCFD2
His27-Gln145 (predicted)
Accession # Q8K5B2

Specificity

Detects mouse SDNSF/MCFD2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human SDNSF is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse SDNSF/MCFD2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse brain

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SDNSF/MCFD2

SDNSF, also known as MCFD2 (multiple coagulation factor deficiency 2), is a secreted molecule from adult hippocampal neural stem/progenitor cells (ANSC) that functions as an autocrine/paracrine factor to maintain neurogenesis in the central nervous system. It is also a component of the MCDF2-LMAN1 complex that functions as a specific cargo receptor for the ER to Golgi transport of proteins. Mutations in MCFD2 causes factor 5 and factor 8 combined deficiency.

Long Name

Stem Cell-derived Neuronal Stem/Progenitor Cell Supporting Factor

Alternate Names

MCFD2

Entrez Gene IDs

90411 (Human); 193813 (Mouse)

Gene Symbol

MCFD2

UniProt

Q8K5B2

Additional SDNSF/MCFD2 Products

Product Documents for Mouse SDNSF/MCFD2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse SDNSF/MCFD2 Antibody

For research use only

Related Research Areas

Coagulation

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Protocols

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FAQs

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