Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all SDNSF/MCFD2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SDNSF/MCFD2
Met1-Gln145
Accession # Q8K5B2

Specificity

Detects mouse SDNSF/MCFD2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse SDNSF/MCFD2 Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse brain

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse SDNSF/MCFD2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SDNSF/MCFD2

SDNSF, also known as MCFD2 (multiple coagulation factor deficiency 2), was described as a secreted molecule from adult hippocampal neural stem/progenitor cells (ANSC) that functions as an autocrine/paracrine factor to maintain neurogenesis in the central nervous system. It is also a component of the MCDF2-LMAN1 (Mannose-binding lectin-1, also known as ERGIC-53) complex that functions as a specific cargo receptor for the ER to golgi transport of proteins. Mutations in MCFD2 causes factor 5 and factor 8 combined deficiency. Human SDNSF shares 84% and 82% amino acid sequence identity with rat and mouse SDNSF, respectively.

Long Name

Stem Cell-derived Neuronal Stem/Progenitor Cell Supporting Factor

Alternate Names

MCFD2

Entrez Gene IDs

90411 (Human); 193813 (Mouse)

Gene Symbol

MCFD2

UniProt

Q8K5B2

Additional SDNSF/MCFD2 Products

Product Documents for Mouse SDNSF/MCFD2 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse SDNSF/MCFD2 Biotinylated Antibody

For research use only

Related Research Areas

Coagulation

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Protocols

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