Mouse SDNSF/MCFD2 Antibody

Catalog # Availability Size / Price Qty
Mouse SDNSF/MCFD2 Antibody in Immunohistochemistry
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Product Details
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Mouse SDNSF/MCFD2 Antibody Summary

Species Reactivity
Detects mouse SDNSF/MCFD2 in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse SDNSF/MCFD2
Accession # Q8K5B2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse SDNSF/MCFD2
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry SDNSF/MCFD2 in Embryonic Mouse Brain. View Larger

SDNSF/MCFD2 in Embryonic Mouse Brain. SDNSF/MCFD2 was detected in immersion fixed frozen sections of embryonic mouse brain (E10, neural tube) using Goat Anti-Mouse SDNSF/MCFD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2556) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to secretion. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SDNSF/MCFD2

SDNSF, also known as MCFD2 (multiple coagulation factor deficiency 2), was described as a secreted molecule from adult hippocampal neural stem/progenitor cells (ANSC) that functions as an autocrine/paracrine factor to maintain neurogenesis in the central nervous system. It is also a component of the MCDF2-LMAN1 (Mannose-binding lectin-1, also known as ERGIC-53) complex that functions as a specific cargo receptor for the ER to golgi transport of proteins. Mutations in MCFD2 causes factor 5 and factor 8 combined deficiency. Human SDNSF shares 84% and 82% amino acid sequence identity with rat and mouse SDNSF, respectively.

Long Name
Stem Cell-derived Neuronal Stem/Progenitor Cell Supporting Factor
Entrez Gene IDs
90411 (Human); 193813 (Mouse)
Alternate Names
DKFZp686G21263; F5F8D2; LMAN1IP; MCFD2; multiple coagulation factor deficiency 2; multiple coagulation factor deficiency protein 2; neural stem cell derived neuronal survival protein; Neural stem cell-derived neuronal survival protein; SDNSF; SDNSFF5F8D

Product Datasheets


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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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