Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Blockade of Receptor-ligand Interaction

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all SECTM1A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SECTM1A
Met1-Thr165
Accession # Q921W8

Specificity

Detects mouse SECTM1A in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse SECTM1A Antibody

SECTM1A antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

SECTM1A in Mouse Embryo.

SECTM1A was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse SECTM1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7837) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse SECTM1A Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.2-1 µg/mL of this antibody will block 50% of the binding of 0.1 µg/mL of Recombinant Mouse SECTM1A Fc Chimera (Catalog # 7837-ST) to immobilized Recombinant Mouse CD7 (Catalog # 8145-CD) coated at 1 µg/mL (100 µL/well).

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SECTM1A

SECTM1A (Secreted and Transmembrane 1A), is 192 amino acid (aa) protein that shares approximately 45‑51%, 81%, and 43% aa sequence identity with human SECTM1 (also called K12), rat SECTM1, and mouse SECTM1B/K12, respectively, and appears to share structural and functional characteristics with other SECTM1 proteins. Human SECTM1 can be found either found as an approximately 27 kDa intracellular type I transmembrane protein that shows a perinuclear, Golgi‑like staining pattern, or as a 20 kDa soluble, secreted form, and is produced by some myeloid cells and by thymic epithelia and fibroblasts (1‑3). Stimulation with IFN‑ gamma is often necessary to detect human SECTM1 expression, and it is thought to be an interferon early‑response gene (1‑5). Mouse SECTM1A cDNA encodes a signal sequence, an extracellular domain with four potential N‑linked glycosylation sites, a transmembrane sequence, and a very short (approximately 6 aa) cytoplasmic sequence (6). SECTM1 proteins from human and mouse show species‑specific binding of CD7 and co‑stimulation of T cells, including enhancement of CD3‑induced proliferation (2‑4).

References

  1. Slentz-Kesler, K.A. et al. (1998) Genomics 47:327.
  2. Lam, G.K. et al. (2005) J. Clin. Immunol. 25:41.
  3. Wang, T. et al. (2012) J. Leukoc. Biol. 91:449.
  4. Lyman, S.D. et al. (2000) J. Biol. Chem. 275:3431.
  5. Huyton, T. et al. (2011) Biochim. Biophys. Acta 1810:1294.
  6. Swiss-Prot Accession # Q921W8.

Long Name

Secreted and Transmembrane 1A

Alternate Names

K12, SECTM

Entrez Gene IDs

209588 (Mouse); 287885 (Rat)

Gene Symbol

SECTM1A

UniProt

Q921W8

Additional SECTM1A Products

Product Documents for Mouse SECTM1A Antibody

Certificate of Analysis

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Product Specific Notices for Mouse SECTM1A Antibody

For research use only

Related Research Areas

NFkB Pathway

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Protocols

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