Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 367612
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Serpin E1/PAI‑1
Leu24-Ser402
Accession # AAH54091

Specificity

Detects mouse Serpin E1/PAI‑1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Serpin A1, A5, A6, B8, C1, D1, E2, F1, F2, I1, recombinant human Serpin A3, A4, B6, B9, G1, or I2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Serpin E1/PAI‑1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Serpin E1/PAI‑1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin E1/PAI-1

Serpin E1, also known as Plasminogen Activator Inhibitor-1 (PAI-1), is the principal inhibitor of urokinase-type and tissue-type plasminogen activators. It plays a major role in many processes such as angiogenesis, tumor invasion, and obesity. The amino acid sequence of mouse PAI-1 is 89%, 79% and 76% identical to that of rat, human/chimpanzee and dog.

Long Name

Plasminogen Activator Inhibitor

Alternate Names

Nexin, PAI-1, PAI1, PLANH1

Entrez Gene IDs

5054 (Human); 18787 (Mouse)

Gene Symbol

SERPINE1

UniProt

Additional Serpin E1/PAI-1 Products

Product Documents for Mouse Serpin E1/PAI‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Serpin E1/PAI‑1 Antibody

For research use only

Citations for Mouse Serpin E1/PAI‑1 Antibody

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