Mouse Serum Amyloid A1/A2 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2948
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Mouse Serum Amyloid A1/A2 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Serum Amyloid A1/A2 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human Serum Amyloid A1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Serum Amyloid A1
Gly20-Tyr122
Accession # P05366
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Serum Amyloid A1/A2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serum Amyloid A1/A2

Mouse Serum Amyloid A1 (SAA1) is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines. It is secreted as a 12 kDa, 103 amino acid (aa), nonglycosylated polypeptide and circulates as part of the HDL complex. The SAA1 gene is one of three SAA genes in mouse, and, based on human, is likely to be allelic. The SAA1 gene product differs from the SAA2 gene product by only nine amino acids. In human, circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one to three amino acids being cleaved from either the N- or C-terminus. The same situation may exist in mouse. The amino acid sequence of mature mouse SAA1 is 72%, 72%, 67% and 74% identical to mature human, rabbit, equine and hamster SAA1, respectively.

Alternate Names
Serum Amyloid A1/A2

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