Mouse Serum Amyloid A1/A2 Antibody

Catalog # Availability Size / Price Qty
AF2948
AF2948-SP
Detection  of Mouse Serum Amyloid A1/A2 by Western Blot.
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Product Details
Citations (7)
FAQs
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Reviews (1)

Mouse Serum Amyloid A1/A2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Serum Amyloid A1/SAA1 and mouse Serum Amyloid A2/SAA2 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) SAA1, rhSAA2, recombinant mouse (rm) SAA3, and rmSAA4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Serum Amyloid A1
Gly20-Tyr122
Accession # P05366
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below
ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB2948).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse Serum Amyloid A DuoSet ELISA Kit (Catalog # DY2948-05) for convenient development of a sandwich ELISA or the Mouse Serum Amyloid A Quantikine ELISA Kit (Catalog # MSAA00) for a complete optimized ELISA.

 
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Mouse Serum Amyloid A1/A2 by Western Blot. View Larger

Detection of Mouse Serum Amyloid A1/A2 by Western Blot. Western blot shows mouse serum and mouse plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serum Amyloid A1/A2 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection  of Mouse Serum Amyloid A1/A2 by Western Blot. View Larger

Detection of Mouse Serum Amyloid A1/A2 by Western Blot. Western blot shows recombinant mouse Serum Amyloid A1 and recombinant mouse Serum Amyloid A2. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serum Amyloid A1/A2 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Serum Amyloid A1/A2 in Mouse Liver. View Larger

Serum Amyloid A1/A2 in Mouse Liver. Serum Amyloid A1/A2 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection  of Mouse Serum Amyloid A1/A2 by Simple  WesternTM. View Larger

Detection of Mouse Serum Amyloid A1/A2 by Simple WesternTM. Simple Western lane view shows mouse serum, loaded at a 1:100 dilution. A specific band was detected for Serum Amyloid A1/A2 at approximately 14 kDa (as indicated) using 50 µg/mL of (Catalog # AF2948) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Mouse Serum Amyloid A1/A2 ELISA Standard Curve. Recombinant Mouse Serum Amyloid A1/A2 protein was serially diluted 2-fold and captured by Rat Anti-Mouse Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB2948) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serum Amyloid A1/A2

Mouse serum amyloid A protein-1 (SAA1) is a multifunctional apolipoprotein produced by hepatocytes in response to pro-inflammatory cytokines. It is secreted as a 12 kDa, 103 amino acid (aa), nonglycosylated polypeptide and circulates as part of the HDL complex. The SAA1 gene is one of three SAA genes in mouse, and, based on human, is likely to be allelic. The SAA1 gene product differs from the SAA2 gene product by only nine amino acids. In human, circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one to three amino acids being cleaved from either the N- or C-terminus. The same situation may exist in mouse. The amino acid sequence of mature mouse SAA1 is 72%, 72%, 67%, and 74% identical to mature human, rabbit, equine, and hamster SAA1, respectively.

Alternate Names
Serum Amyloid A1/A2

Product Datasheets

Citations for Mouse Serum Amyloid A1/A2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Increased hypothalamic microglial activation after viral-induced pneumococcal lung infection is associated with excess serum amyloid A production
    Authors: H Wang, M Blackall, L Sominsky, SJ Spencer, R Vlahos, M Churchill, S Bozinovski
    J Neuroinflammation, 2018;15(1):200.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Amyloid deposition in a mouse model humanized at the transthyretin and retinol-binding protein 4 loci
    Authors: X Li, Y Lyu, J Shen, Y Mu, L Qiang, L Liu, K Araki, BP Imbimbo, KI Yamamura, S Jin, Z Li
    Lab. Invest., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Scavenger receptor A1 prevents metastasis of non-small cell lung cancer via suppression of macrophage serum amyloid A1
    Authors: Y Zhang, Y Wei, B Jiang, L Chen, H Bai, X Zhu, X Li, H Zhang, Q Yang, J Ma, Y Xu, J Ben, DC Christiani, Q Chen
    Cancer Res, 2017;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Zymosan-mediated inflammation impairs in vivo reverse cholesterol transport.
    Authors: Malik P, Berisha SZ, Santore J
    J. Lipid Res., 2011;52(5):951-7.
    Species: Mouse
    Sample Types: Plasma
    Applications: WB
  5. Transmission of circulating cell-free AA amyloid oligomers in exosomes vectors via a prion-like mechanism.
    Authors: Tasaki M, Ueda M, Ochiai S, Tanabe Y, Murata S, Misumi Y, Su Y, Sun X, Shinriki S, Jono H, Shono M, Obayashi K, Ando Y
    Biochem. Biophys. Res. Commun., 2010;400(4):559-62.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: WB
  6. Fish oil increases cholesterol storage in white adipose tissue with concomitant decreases in inflammation, hepatic steatosis, and atherosclerosis in mice.
    Authors: Saraswathi V, Gao L, Morrow JD, Chait A, Niswender KD, Hasty AH
    J. Nutr., 2007;137(7):1776-82.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  7. Role of APAF-1, E-cadherin and peritumoral lymphocytic infiltration in tumour budding in colorectal cancer.
    Authors: Zlobec I, Lugli A, Baker K, Roth S, Minoo P, Hayashi S, Terracciano L, Jass JR
    J. Pathol., 2007;212(3):260-8.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development

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Reviews for Mouse Serum Amyloid A1/A2 Antibody

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Mouse Serum Amyloid A1 Antibody
By Anonymous on 10/14/2016
Application: WB Sample Tested: Serum,Recombinant protein Species: Mouse

Protein loaded:

Lane 1: SAA1 50ng
Lane 2: SAA2 50ng
Lane 3: SAA1 10ng
Lane 4: SAA2 10ng
Lane 5: 50nl serum, LPS-treated mouse
Lane 6: 10nl serum, LPS-treated mouse

Recombinant proteins were from R and D systems

Prepared in Invitrogen 1x LDS sample buffer plus 1x reducing agent.

Run on 4-12% Bis-tris gel under reducing conditions in MOPS buffer. Transferred using iBlot 20V 7 min
Blocked 1 hour 5% BSA/TBS-T
Incubated overnight 4 C, antibody prepared 1/1000 in 5% BSA/TBS-T
Secondary antibody was anti-goat from Jackson Immuno 1:5000 for 30 minutes.
West Pico Chemiluminescent Reagent
ImageQuant LAS4000 for imaging

*This antibody gives great signal, but predominantly detects SAA2 over SAA1.