Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Immunocytochemistry, Cell Culture, ELISA Capture, ELISA Development, In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Serum Amyloid A1/A2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Serum Amyloid A1
Gly20-Tyr122
Accession # P05366

Specificity

Detects mouse Serum Amyloid A1/SAA1 and mouse Serum Amyloid A2/SAA2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Serum Amyloid A1/A2 Antibody

Detection of Mouse Serum Amyloid A1/A2 antibody by Western Blot.

Detection of Mouse Serum Amyloid A1/A2 by Western Blot.

Western blot shows mouse serum and mouse plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serum Amyloid A1/A2 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Serum Amyloid A1/A2 antibody by Western Blot.

Detection of Mouse Serum Amyloid A1/A2 by Western Blot.

Western blot shows recombinant mouse Serum Amyloid A1 and recombinant mouse Serum Amyloid A2. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serum Amyloid A1/A2 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Serum Amyloid A1/A2 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr).

Serum Amyloid A1/A2 in Mouse Liver.

Serum Amyloid A1/A2 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse Serum Amyloid A1/A2 antibody by Simple WesternTM.

Detection of Mouse Serum Amyloid A1/A2 by Simple WesternTM.

Simple Western lane view shows mouse serum, loaded at a 1:100 dilution. A specific band was detected for Serum Amyloid A1/A2 at approximately 14 kDa (as indicated) using 50 µg/mL of (Catalog # AF2948) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Mouse Serum Amyloid A1/A2 Antibody in ELISA Standard Curve.

Mouse Serum Amyloid A1/A2 ELISA Standard Curve.

Recombinant Mouse Serum Amyloid A1/A2 protein was serially diluted 2-fold and captured by Rat Anti-Mouse Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB2948) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Detection of Serum Amyloid A1/A2 by Western Blot

Detection of Serum Amyloid A1/A2 by Western Blot

Robust expression of acute phase response proteins in skeletal muscle versus liver in C26 cachexia.A. Western blotting and quantitation of fibrinogen levels in control and C26 quadriceps and liver. Data (mean ± SEM) are expressed as relative densitometry value. **P<0.01, ***P<0.001. B, Western blotting analysis of fibrinogen standard proteins and quadriceps and liver extracts for control, CHO-IL6 injected nude mice and C26 injected CD2F1 mice. Quantitation was performed on the band indicated by the arrow. Data (means ± SEM) are expressed as ng fibrinogen / µg protein. *P<0.05, **P<0.01, ***P<0.001. C, Western blotting analysis demonstrates significantly increased fibrinogen and SAA1 protein levels in quadriceps and gastrocnemius in moderate and severe C26 cachexia. *P<0.05, **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/21799891), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Serum Amyloid A1/A2 by Western Blot

Detection of Serum Amyloid A1/A2 by Western Blot

FMT-AD neither induced peripheral inflammation nor caused intestinal damage to recipient mice. (a) Western blot showing protein Serum Amyloid A (SAA) levels in the serum from FMT-AD and FMT-young (FMT-y) mice at 3 d post-TBI. (b) Quantification of the western blot shows a not statistically significant increase in SAA in the serum of males after FMT-AD compared to FMT-y. (c) Representative histology images from intestinal tissue do not show any abnormality between the FMT-y and FMT-AD groups in male or female mice. Left, Hematoxylin and eosin (H.E.) staining; right (×10 Objective), Alcian blue staining (×20 Objective). (d) Densiometric quantification of the Alcian blue positive area and counting the number of goblet cells per villus are not different between FMT-y and FMT-AD groups. Male and female data were combined, since two-way ANOVA showed no sex differences (Alcian blue area, p = 0.429; goblet cells, p = 0.471). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35562867), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Serum Amyloid A1/A2 by Western Blot

Detection of Serum Amyloid A1/A2 by Western Blot

FMT-AD neither induced peripheral inflammation nor caused intestinal damage to recipient mice. (a) Western blot showing protein Serum Amyloid A (SAA) levels in the serum from FMT-AD and FMT-young (FMT-y) mice at 3 d post-TBI. (b) Quantification of the western blot shows a not statistically significant increase in SAA in the serum of males after FMT-AD compared to FMT-y. (c) Representative histology images from intestinal tissue do not show any abnormality between the FMT-y and FMT-AD groups in male or female mice. Left, Hematoxylin and eosin (H.E.) staining; right (×10 Objective), Alcian blue staining (×20 Objective). (d) Densiometric quantification of the Alcian blue positive area and counting the number of goblet cells per villus are not different between FMT-y and FMT-AD groups. Male and female data were combined, since two-way ANOVA showed no sex differences (Alcian blue area, p = 0.429; goblet cells, p = 0.471). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35562867), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Serum Amyloid A1/A2 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB2948).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse Serum Amyloid A DuoSet ELISA Kit (Catalog # DY2948-05) for convenient development of a sandwich ELISA or the Mouse Serum Amyloid A Quantikine ELISA Kit (Catalog # MSAA00) for a complete optimized ELISA.

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver

Simple Western

50 µg/mL
Sample:   Mouse serum

Western Blot

1 µg/mL
Sample: Mouse serum and mouse plasma

Reviewed Applications

Read 3 reviews rated 3.7 using AF2948 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serum Amyloid A1/A2

Mouse serum amyloid A protein-1 (SAA1) is a multifunctional apolipoprotein produced by hepatocytes in response to pro-inflammatory cytokines. It is secreted as a 12 kDa, 103 amino acid (aa), nonglycosylated polypeptide and circulates as part of the HDL complex. The SAA1 gene is one of three SAA genes in mouse, and, based on human, is likely to be allelic. The SAA1 gene product differs from the SAA2 gene product by only nine amino acids. In human, circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one to three amino acids being cleaved from either the N- or C-terminus. The same situation may exist in mouse. The amino acid sequence of mature mouse SAA1 is 72%, 72%, 67%, and 74% identical to mature human, rabbit, equine, and hamster SAA1, respectively.

Alternate Names

PIG4;SAA;SAA1;SAA2;Serum amyloid A-1 protein;Serum amyloid A-2 protein;TP53I4

UniProt

P05366

Additional Serum Amyloid A1/A2 Products

Product Documents for Mouse Serum Amyloid A1/A2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Serum Amyloid A1/A2 Antibody

For research use only

Citations for Mouse Serum Amyloid A1/A2 Antibody

Customer Reviews for Mouse Serum Amyloid A1/A2 Antibody (3)

3.7 out of 5
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Showing  1 - 3 of 3 reviews Showing All
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  • Mouse Serum Amyloid A1/A2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Spinal cord
    Species: Mouse
    Verified Customer | Posted 12/24/2021
    Mouse Serum Amyloid A1/A2 Antibody AF2948
  • Mouse Serum Amyloid A1/A2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 10/17/2018
  • Mouse Serum Amyloid A1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Serum and Recombinant protein
    Species: Mouse
    Verified Customer | Posted 10/14/2016
    Protein loaded: Lane 1: SAA1 50ng Lane 2: SAA2 50ng Lane 3: SAA1 10ng Lane 4: SAA2 10ng Lane 5: 50nl serum, LPS-treated mouse Lane 6: 10nl serum, LPS-treated mouse Recombinant proteins were from R and D systems Prepared in Invitrogen 1x LDS sample buffer plus 1x reducing agent. Run on 4-12% Bis-tris gel under reducing conditions in MOPS buffer. Transferred using iBlot 20V 7 min Blocked 1 hour 5% BSA/TBS-T Incubated overnight 4 C, antibody prepared 1/1000 in 5% BSA/TBS-T Secondary antibody was anti-goat from Jackson Immuno 1:5000 for 30 minutes. West Pico Chemiluminescent Reagent ImageQuant LAS4000 for imaging *This antibody gives great signal, but predominantly detects SAA2 over SAA1.
    Mouse Serum Amyloid A1/A2 Antibody AF2948

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