Mouse Serum Amyloid A1/A2 Antibody AF2948: R&D Systems

Mouse Serum Amyloid A1/A2 Antibody

(5 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Serum Amyloid A1/SAA1 and mouse Serum Amyloid A2/SAA2 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) SAA1, rhSAA2, recombinant mouse (rm) SAA3, and rmSAA4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse Serum Amyloid A1
    Gly20-Tyr122
    Accession # P05366
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    50 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Serum Amyloid A1/A2 by Western Blot. Western blot shows mouse serum and mouse plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serum Amyloid A1/A2 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Serum Amyloid A1/A2 by Western Blot. Western blot shows recombinant mouse Serum Amyloid A1 and recombinant mouse Serum Amyloid A2. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serum Amyloid A1/A2 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Serum Amyloid A1/A2 in Mouse Liver. Serum Amyloid A1/A2 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse Serum Amyloid A1/A2 by Simple WesternTM. Simple Western lane view shows mouse serum, loaded at a 1:100 dilution. A specific band was detected for Serum Amyloid A1/A2 at approximately 14 kDa (as indicated) using 50 µg/mL of (Catalog # AF2948) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serum Amyloid A1/A2
Mouse serum amyloid A protein-1 (SAA1) is a multifunctional apolipoprotein produced by hepatocytes in response to pro-inflammatory cytokines. It is secreted as a 12 kDa, 103 amino acid (aa), nonglycosylated polypeptide and circulates as part of the HDL complex. The SAA1 gene is one of three SAA genes in mouse, and, based on human, is likely to be allelic. The SAA1 gene product differs from the SAA2 gene product by only nine amino acids. In human, circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one to three amino acids being cleaved from either the N- or C-terminus. The same situation may exist in mouse. The amino acid sequence of mature mouse SAA1 is 72%, 72%, 67%, and 74% identical to mature human, rabbit, equine, and hamster SAA1, respectively.
  • Alternate Names:
    Serum Amyloid A1/A2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Scavenger receptor A1 prevents metastasis of non-small cell lung cancer via suppression of macrophage serum amyloid A1
    Authors: Y Zhang, Y Wei, B Jiang, L Chen, H Bai, X Zhu, X Li, H Zhang, Q Yang, J Ma, Y Xu, J Ben, DC Christiani, Q Chen
    Cancer Res, 2017;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  2. Zymosan-mediated inflammation impairs in vivo reverse cholesterol transport.
    Authors: Malik P, Berisha SZ, Santore J
    J. Lipid Res., 2011;52(5):951-7.
    Species: Mouse
    Sample Type: Plasma
    Application: WB
  3. Transmission of circulating cell-free AA amyloid oligomers in exosomes vectors via a prion-like mechanism.
    Authors: Tasaki M, Ueda M, Ochiai S, Tanabe Y, Murata S, Misumi Y, Su Y, Sun X, Shinriki S, Jono H, Shono M, Obayashi K, Ando Y
    Biochem. Biophys. Res. Commun., 2010;400(4):559-62.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  4. Role of APAF-1, E-cadherin and peritumoral lymphocytic infiltration in tumour budding in colorectal cancer.
    Authors: Zlobec I, Lugli A, Baker K, Roth S, Minoo P, Hayashi S, Terracciano L, Jass JR
    J. Pathol., 2007;212(3):260-8.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Development
  5. Fish oil increases cholesterol storage in white adipose tissue with concomitant decreases in inflammation, hepatic steatosis, and atherosclerosis in mice.
    Authors: Saraswathi V, Gao L, Morrow JD, Chait A, Niswender KD, Hasty AH
    J. Nutr., 2007;137(7):1776-82.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Development
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

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Immunohistochemistry Reagents
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Isotype Controls
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Normal Goat IgG Control

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Secondary Antibodies
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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Mouse Serum Amyloid A1 Antibody AF2948
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Very Good
 WB Mouse Anonymous 10/14/2016
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Western Blot Mouse Serum Amyloid A1 Antibody AF2948
Western Blot: Mouse Serum Amyloid A1 Antibody [AF2948]

Summary

ApplicationWestern Blot
Sample TestedSerum,Recombinant protein
SpeciesMouse

Other Experimental Details

Other Experimental DetailsProtein loaded:Lane 1: SAA1 50ngLane 2: SAA2 50ngLane 3: SAA1 10ngLane 4: SAA2 10ngLane 5: 50nl serum, LPS-treated mouseLane 6: 10nl serum, LPS-treated mouseRecombinant proteins were from R and D systemsPrepared in Invitrogen 1x LDS sample buffer plus 1x reducing agent.Run on 4-12% Bis-tris gel under reducing conditions in MOPS buffer. Transferred using iBlot 20V 7 minBlocked 1 hour 5% BSA/TBS-TIncubated overnight 4 C, antibody prepared 1/1000 in 5% BSA/TBS-TSecondary antibody was anti-goat from Jackson Immuno 1:5000 for 30 minutes.West Pico Chemiluminescent ReagentImageQuant LAS4000 for imaging*This antibody gives great signal, but predominantly detects SAA2 over SAA1.

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