Mouse Serum Amyloid A1/A2 Antibody
Mouse Serum Amyloid A1/A2 Antibody Summary
Accession # P05366
This antibody functions as an ELISA capture antibody when paired with Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse Serum Amyloid A DuoSet ELISA Kit (Catalog # DY2948-05) for convenient development of a sandwich ELISA or the Mouse Serum Amyloid A Quantikine ELISA Kit (Catalog # MSAA00) for a complete optimized ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Mouse Serum Amyloid A1/A2 ELISA Standard Curve. Recombinant Mouse Serum Amyloid A1/A2 protein was serially diluted 2-fold and captured by Rat Anti-Mouse Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB2948) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serum Amyloid A1/A2
Mouse Serum Amyloid A Protein-1 (SAA1) is a multifunctional apolipoprotein produced by hepatocytes in response to pro-inflammatory cytokines. It is secreted as a 12 kDa, 103 amino acid (aa), nonglycosylated polypeptide and circulates as part of the HDL complex. The SAA1 gene is one of three SAA genes in mouse, and, based on human, is likely to be allelic. The SAA1 gene product differs from the SAA2 gene product by only nine amino acids. In human, circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one to three amino acids being cleaved from either the N- or C-terminus. The same situation may exist in mouse. The amino acid sequence of mature mouse SAA1 is 72%, 72%, 67%, and 74% identical to mature human, rabbit, equine, and hamster SAA1, respectively.
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