Detection of Mouse Shrew-1/AJAP1 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue, mouse brain (thalamus/hypothalamus) tissue, and mouse pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Shrew-1/AJAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7970) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Shrew-1/ AJAP1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
AJAP-1 (Adherens Junction Associated Protein 1; also Shrew-1 and Gm573) is a 44-47 kDa transmembrane protein found on the basolateral surface of epithelium. It is presumably expressed by multiple types of epithelium, and participates in cell-cell adhesion. Zonula adherens are structures that participate in the generation of cell contact adherens junctions. Such junctions, or complexes, are key to the maintenance of epithelial cell polarity and the maintenance of cell-to-cell contact. Central to this complex is E-cadherin, a transmembrane molecule that makes crucial contacts with cytoskeletal actin via catenins. AJAP-1 is an accompaning transmembrane molecule that interacts with E-cadherin and appears to promote the internalization of E-cadherin upon growth factor stimulation. This facilitates the dissolution of adherens junctions with an increase in cell motility. Notably, AJAP-1 is also known to complex with CD147 in nonpolar, invasive cells, suggesting that AJAP-1 may play a more complex role in cell migration. Mouse AJAP-1 is a 412 amino acid (aa) type III (no signal sequence) transmembrane protein. It contains a 284 aa extracellular region (aa 1-284) plus a 106 aa cytoplasmic domain (aa 306-412). Over aa 1-283, mouse AJAP-1 shares 95% and 75% aa sequence identity with rat and human AJAP-1, respectively; over aa 158-283, mouse AJAP-1 sequence identity changes little, showing 95% and 78% equality with rat and human AJAP-1, respectively.
Transmembrane Protein Shrew-1/Adherens Junction Associated Protein 1
Entrez Gene IDs:
55966 (Human); 230959 (Mouse)
adherens junction associated protein 1; adherens junction-associated protein 1; adherens junctions associated protein 1; AJAP1; Gm573; membrane protein shrew-1; MOT8; RP3-426F10.1; Shrew1; Shrew-1; transmembrane protein SHREW1
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