Mouse Shrew-1/AJAP1 Antibody

Catalog # Availability Size / Price Qty
AF7970
AF7970-SP
Detection of Mouse Shrew-1/AJAP1 by Western Blot.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Mouse Shrew-1/AJAP1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Shrew-1/AJAP1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human AJAP1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Shrew-1/AJAP1
His157-His283
Accession # A2ALI5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse Shrew-1/AJAP1 by Western Blot. View Larger

Detection of Mouse Shrew-1/AJAP1 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue, mouse brain (thalamus/hypothalamus) tissue, and mouse pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Shrew-1/AJAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7970) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Shrew-1/
AJAP1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Shrew-1/AJAP1

AJAP-1 (Adherens Junction Associated Protein 1; also Shrew-1 and Gm573) is a 44-47 kDa transmembrane protein found on the basolateral surface of epithelium. It is presumably expressed by multiple types of epithelium, and participates in cell-cell adhesion. Zonula adherens are structures that participate in the generation of cell contact adherens junctions. Such junctions, or complexes, are key to the maintenance of epithelial cell polarity and the maintenance of cell-to-cell contact. Central to this complex is E-cadherin, a transmembrane molecule that makes crucial contacts with cytoskeletal actin via catenins. AJAP-1 is an accompaning transmembrane molecule that interacts with E-cadherin and appears to promote the internalization of E-cadherin upon growth factor stimulation. This facilitates the dissolution of adherens junctions with an increase in cell motility. Notably, AJAP-1 is also known to complex with CD147 in nonpolar, invasive cells, suggesting that AJAP-1 may play a more complex role in cell migration. Mouse AJAP-1 is a 412 amino acid (aa) type III (no signal sequence) transmembrane protein. It contains a 284 aa extracellular region (aa 1-284) plus a 106 aa cytoplasmic domain (aa 306-412). Over aa 1-283, mouse AJAP-1 shares 95% and 75% aa sequence identity with rat and human AJAP-1, respectively; over aa 158-283, mouse AJAP-1 sequence identity changes little, showing 95% and 78% equality with rat and human AJAP-1, respectively.

Long Name
Transmembrane Protein Shrew-1/Adherens Junction Associated Protein 1
Entrez Gene IDs
55966 (Human); 230959 (Mouse)
Alternate Names
adherens junction associated protein 1; adherens junction-associated protein 1; adherens junctions associated protein 1; AJAP1; Gm573; membrane protein shrew-1; MOT8; RP3-426F10.1; Shrew1; Shrew-1; transmembrane protein SHREW1

Product Datasheets

Citations for Mouse Shrew-1/AJAP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Complex formation of APP with GABAB receptors links axonal trafficking to amyloidogenic processing
    Authors: MC Dinamarca, A Raveh, A Schneider, T Fritzius, S Früh, PD Rem, M Stawarski, T Lalanne, R Turecek, M Choo, V Besseyrias, W Bildl, D Bentrop, M Staufenbie, M Gassmann, B Fakler, J Schwenk, B Bettler
    Nat Commun, 2019;10(1):1331.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Binding Assay
  2. Alternative exon usage creates novel transcript variants of tumor suppressor SHREW-1 gene with differential tissue expression profile
    Authors: Anna Starzinski
    Biol Open, 2016;5(11):1607-1619.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  3. Modular composition and dynamics of native GABAB receptors identified by high-resolution proteomics.
    Authors: Schwenk J, Perez-Garci E, Schneider A, Kollewe A, Gauthier-Kemper A, Fritzius T, Raveh A, Dinamarca M, Hanuschkin A, Bildl W, Klingauf J, Gassmann M, Schulte U, Bettler B, Fakler B
    Nat Neurosci, 2016;19(2):233-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-Fr

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Mouse Shrew-1/AJAP1 Antibody
By Anonymous on 01/17/2018
Application: IP Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse