Mouse Soggy-1/DkkL1 Antibody

Catalog # Availability Size / Price Qty
AF1508
AF1508-SP
Detection  of Mouse Soggy‑1/DkkL1 by Western Blot.
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Mouse Soggy-1/DkkL1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Soggy-1/DkkL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human Soggy-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Soggy-1/DkkL1
Leu21-Leu230
Accession # AAF02679
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Mouse Soggy‑1/DkkL1 by Western Blot. View Larger

Detection of Mouse Soggy‑1/DkkL1 by Western Blot. Western blot shows lysate of mouse testis tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Soggy‑1/DkkL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1508) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Soggy‑1/DkkL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Soggy-1/DkkL1

Soggy-1, also known as DkkL1 (Dickkopf-like 1), is a secreted protein that is related to the Dickkopf family of Wnt antagonists. Dkk-1, -2, -3, and -4 each have two cysteine-rich domains separated by a linker. Soggy-1 does not contain cysteine-rich domains but bears some sequence homology with the N-terminal regions of Dkk proteins (1, 2). The mouse Soggy-1 cDNA encodes a 230 amino acid (aa) precursor that includes a 20 aa signal sequence (2). Mouse Soggy-1 shares 65% and 90% aa sequence identity with human and rat Soggy-1, respectively, and approximately 15% aa sequence identity with mouse Dkk-1, -2, -3, and -4. Mouse Soggy-1 is expressed at various sites in the embryo but in the adult is primarily found in the testes (3‑5). Soggy-1 transcription is regulated by the spermatocyte specific factor, RFX2 (6). The regulatory elements for Soggy-1 lie very close to those of TEAD-2, a transcription factor that is expressed very early in development (4). Soggy-1 and TEAD-2 are co-expressed in preimplantation embryos and embryonic stem cells, but differentiated cells express only one or the other (4, 5). During development, Soggy-1 is first detectable at the onset of sexual differentiation (4). Soggy-1 is localized to the acrosome in developing spermatocytes and mature spermatozoa (3, 4). Soggy-1, as expressed in developing mouse spermatocytes, is a 34 kDa N-glycosylated protein. This glycosylation is not present on Soggy-1 in mature spermatozoa, although the apparent molecular weight suggests the presence of some post-translational modification (3). Two shorter forms of Soggy-1 have been described that result from the use of internal methionine residues for initiation (4).

References
  1. Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
  2. Krupnik, V.E. et al. (1999) Gene 238:301.
  3. Kohn, M.J. et al. (2005) Mol. Reprod. Dev. 71:516.
  4. Kaneko, K.J. and M.L. DePamphilis (2000) Nucleic Acids Res. 28:3982.
  5. Kaneko, K.J. et al. (2004) Mol. Cell. Biol. 24:1968.
  6. Horvath, G.C. et al. (2004) Biol. Reprod. 71:1551.
Long Name
Dickkopf-like Protein 1
Entrez Gene IDs
27120 (Human); 50722 (Mouse)
Alternate Names
Cancer/testis antigen 34; CT34dickkopf-like 1 (soggy); dickkopf-like 1; DkkL1; Protein soggy-1; SGY; SGY1; SGY-1dickkopf-like protein 1; SGY1soggy; Soggy1; Soggy-1

Product Datasheets

Citations for Mouse Soggy-1/DkkL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. SLXL1, a Novel Acrosomal Protein, Interacts with DKKL1 and Is Involved in Fertilization in Mice.
    Authors: Zhuang XJ, Hou XJ, Liao SY
    PLoS ONE, 2011;6(6):e20866.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  2. DkkL1 (Soggy), a Dickkopf family member, localizes to the acrosome during mammalian spermatogenesis.
    Authors: Kohn MJ, Kaneko KJ, DePamphilis ML
    Mol. Reprod. Dev., 2005;71(4):516-22.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse Soggy-1/DkkL1 Antibody

There are currently no reviews for this product. Be the first to review Mouse Soggy-1/DkkL1 Antibody and earn rewards!

Have you used Mouse Soggy-1/DkkL1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review