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Mouse Stella/Dppa3 Biotinylated Antibody

Catalog #: BAF2566 Datasheet / COA / SDS
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BAF2566

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Mouse Stella/Dppa3 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Stella/Dppa3 in Western blots. In Western blots, approximately 15% cross‑reactivity with recombinant human Stella is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Stella/Dppa3
Met1-Asn150
Accession # Q8QZY3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Stella/Dppa3
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse ovary

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Stella/Dppa3

Stella, also known as Dppa3 (Developmental pluripotency-associated 3) and PGC7 (primodial germ cell 7) is expressed in primodial germ cells, oocytes, preimplantation embryos, and pluripotent cells. Although the fuction of Stella is not known, the expression pattern of the protein suggests a role in the maintenance of cell pluripotency.

Long Name
Developmental Pluripotency-associated Protein 3
Entrez Gene IDs
359787 (Human); 73708 (Mouse)
Alternate Names
developmental pluripotency associated 3; DPPA3 developmental pluripotency associated 3; Dppa3; Stella

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