Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Stella/Dppa3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Stella/Dppa3
Met1-Asn150
Accession # Q8QZY3

Specificity

Detects mouse Stella/Dppa3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant human Stella is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Stella/Dppa3 Antibody

Stella/Dppa3 antibody in mouse ovary tissue by Immunohistochemistry (IHC-Fr).

Stella/Dppa3 in mouse ovary tissue.

Stella/Dppa3 was detected in perfusion fixed frozen sections of mouse ovary tissue using Goat Anti-Mouse Stella/Dppa3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2566) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to oocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse Stella/Dppa3 Antibody

Application
Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse ovary

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Stella/Dppa3

Reviewed Applications

Read 2 reviews rated 4.5 using AF2566 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Stella/Dppa3

Stella, also known as Dppa3 (Developmental pluripotency-associated 3) and PGC7 (primordial germ cell 7) is expressed in primordial germ cells, oocytes, preimplantation embryos, and pluripotent cells. Although the fuction of Stella is not known, the expression pattern of the protein suggests a role in the maintenance of cell pluripotency.

Long Name

Developmental Pluripotency-associated Protein 3

Alternate Names

Dppa3

Entrez Gene IDs

359787 (Human); 73708 (Mouse)

Gene Symbol

DPPA3

UniProt

Q8QZY3

Additional Stella/Dppa3 Products

Product Documents for Mouse Stella/Dppa3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Stella/Dppa3 Antibody

For research use only

Related Research Areas

Primordial and Mature Germ Cells

Citations for Mouse Stella/Dppa3 Antibody

Customer Reviews for Mouse Stella/Dppa3 Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
50%
3 Stars
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1 Stars
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Customer Images

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  • Mouse Stella/Dppa3 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Ovary tissue
    Species: Mouse
    Verified Customer | Posted 11/15/2024
    Working concentration 1:200, IHC-P
    Mouse Stella/Dppa3 Antibody AF2566
  • Mouse Stella/Dppa3 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Ovary tissue
    Species: Mouse
    Verified Customer | Posted 11/08/2024
    The antibody was tested on a paraffin-embedded section of a mouse P14 ovary, with successful staining observed at a dilution of 1:200. Yellow (Nr2F2), Magenta (Stella)
    Mouse Stella/Dppa3 Antibody AF2566

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Protocols

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