Mouse sVCAM-1/CD106 Quantikine ELISA Kit

(17 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL)
  • Sensitivity
    0.06 ng/mL
  • Assay Range
    0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant mouse sVCAM-1
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse soluble VCAM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse sVCAM-1 levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant mouse sVCAM-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse sVCAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse sVCAM-1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean0.962.848.550.872.648.5
Standard Deviation0.050.130.370.070.180.63
CV%5.24.64.386.87.4

Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean0.932.618.580.862.468.46
Standard Deviation0.070.170.40.050.110.65
CV%7.56.54.75.84.57.7

Recovery

The recovery of mouse sVCAM-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 100 89-112
EDTA Plasma (n=4) 100 81-119
Serum (n=10) 99 80-118
Linearity
To assess the linearity of the assay, six samples of each matrix containing and/or spiked with high concentrations of mouse sVCAM-1 were diluted with Calibrator Diluent and then assayed.
Mouse sVCAM-1/CD106 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: VCAM-1/CD106
VCAM-1 (Vascular Cell Adhesion Molecule-1; CD106) is a transmembrane molecule that mediates the adhesion of immune cells to the vascular endothelium during inflammation. It binds to several integrins including alpha4/beta1 (VLA-4), alpha4/beta7, alpha9/beta1, and alphaD/beta2. It is expressed constitutively in the bone marrow where it regulates T cell, B cell, and hematopoietic progenitor cell migration. A soluble form VCAM-1 can promote monocyte chemotaxis.
    • Long Name
      Vascular Cell Adhesion Molecule 1
    • Entrez Gene IDs
      7412 (Human); 22329 (Mouse);
    • Alternate Names
      CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of the appropriate Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    17 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Soluble Vascular Cell Adhesion Molecule-1 (VCAM-1) as a Biomarker in the Mouse Model of Experimental Autoimmune Myocarditis (EAM)
      PLoS ONE, 2016;11(8):e0158299.
      Species: Mouse
      Sample Type: Tissue Homogenates
    2. A Critical Role for P2X7 Receptor-Induced VCAM-1 Shedding and Neutrophil Infiltration during Acute Lung Injury
      J Immunol, 2016;0(0):.
      Species: Mouse
      Sample Type: BALF
    3. Reduction of adhesion molecule production and alteration of eNOS and endothelin-1 mRNA expression in endothelium by Euphorbia hirta L. through its beneficial beta-amyrin molecule.
      Authors: Shih M, Cherng J
      Molecules, 2014;19(7):10534-45.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    4. Endothelial mineralocorticoid receptor activation enhances endothelial protein C receptor and decreases vascular thrombosis in mice.
      Authors: Lagrange J, Li Z, Fassot C, Bourhim M, Louis H, Nguyen Dinh Cat A, Parlakian A, Wahl D, Lacolley P, Jaisser F, Regnault V
      FASEB J, 2014;28(5):2062-72.
      Species: Mouse
      Sample Type: Plasma
    5. Early protection from burn-induced acute lung injury by deletion of preprotachykinin-A gene.
      Authors: Sio SW, Moochhala S, Lu J
      Am. J. Respir. Crit. Care Med., 2010;181(1):36-46.
      Species: Mouse
      Sample Type: Tissue Homogenates
    6. Alternative implication of CXCR4 in JAK2/STAT3 activation in small cell lung cancer.
      Authors: Pfeiffer M, Hartmann TN, Leick M, Catusse J, Schmitt-Graeff A, Burger M
      Br. J. Cancer, 2009;100(12):1949-56.
    7. Early atherosclerosis and vascular inflammation in mice with diet-induced type 2 diabetes.
      Authors: Bartels ED, Bang CA, Nielsen LB
      Eur. J. Clin. Invest., 2009;39(3):190-9.
      Species: Mouse
      Sample Type: Plasma
    8. A neutralizing antibody against receptor for advanced glycation end products (RAGE) reduces atherosclerosis in uremic mice.
      Authors: Bro S, Flyvbjerg A, Binder CJ, Bang CA, Denner L, Olgaard K, Nielsen LB
      Atherosclerosis, 2008;201(2):274-80.
      Species: Mouse
      Sample Type: Plasma
    9. Prevention of murine antiphospholipid syndrome by BAFF blockade.
      Authors: Kahn P, Ramanujam M, Bethunaickan R, Huang W, Tao H, Madaio MP, Factor SM, Davidson A
      Arthritis Rheum., 2008;58(9):2824-34.
      Species: Mouse
      Sample Type: Serum
    10. Vascular dysfunction in a murine model of severe hemolysis.
      Authors: Frei AC, Guo Y, Jones DW, Pritchard KA, Fagan KA, Hogg N, Wandersee NJ
      Blood, 2008;112(2):398-405.
      Species: Mouse
      Sample Type: Plasma
    11. Vascular and inflammatory stresses mediate atherosclerosis via RAGE and its ligands in apoE-/- mice.
      Authors: Harja E, Bu DX, Hudson BI, Chang JS, Shen X, Hallam K, Kalea AZ, Lu Y, Rosario RH, Oruganti S, Nikolla Z, Belov D, Lalla E, Ramasamy R, Yan SF, Schmidt AM
      J. Clin. Invest., 2008;118(1):183-94.
      Species: Mouse
      Sample Type: Plasma
    12. Aspergillus fumigatus stimulates leukocyte adhesion molecules and cytokine production by endothelial cells in vitro and during invasive pulmonary disease.
      Authors: Chiang LY, Sheppard DC, Gravelat FN, Patterson TF, Filler SG
      Infect. Immun., 2008;76(8):3429-38.
      Species: Mouse
      Sample Type: Tissue Homogenates
    13. Atheroprotective role of interleukin-6 in diet- and/or pathogen-associated atherosclerosis using an ApoE heterozygote murine model.
      Authors: Madan M, Bishayi B, Hoge M, Amar S
      Atherosclerosis, 2007;197(2):504-14.
      Species: Mouse
      Sample Type: Serum
    14. Cell-based angiopoietin-1 gene therapy for acute lung injury.
      Authors: McCarter SD, Mei SH, Lai PF, Zhang QW, Parker CH, Suen RS, Hood RD, Zhao YD, Deng Y, Han RN, Dumont DJ, Stewart DJ
      Am. J. Respir. Crit. Care Med., 2007;175(10):1014-26.
      Species: Mouse
      Sample Type: Plasma
    15. Ionizing radiation accelerates the development of atherosclerotic lesions in ApoE-/- mice and predisposes to an inflammatory plaque phenotype prone to hemorrhage.
      Authors: Stewart FA, Heeneman S, Te Poele J, Kruse J, Russell NS, Gijbels M, Daemen M
      Am. J. Pathol., 2006;168(2):649-58.
      Species: Mouse
      Sample Type: Plasma
    16. Suppression of graft coronary artery disease by a brief treatment with a selective epsilonPKC activator and a deltaPKC inhibitor in murine cardiac allografts.
      Authors: Tanaka M, Terry RD, Mokhtari GK, Inagaki K, Koyanagi T, Kofidis T, Mochly-Rosen D, Robbins RC
      Circulation, 2004;110(11):II194-9.
      Species: Mouse
      Sample Type: Tissue Homogenates
    17. Cardiomyocyte-specific Bcl-2 overexpression attenuates ischemia-reperfusion injury, immune response during acute rejection, and graft coronary artery disease.
      Authors: Tanaka M, Nakae S, Terry RD, Mokhtari GK, Gunawan F, Balsam LB, Kaneda H, Kofidis T, Tsao PS, Robbins RC
      Blood, 2004;104(12):3789-96.
      Species: Mouse
      Sample Type: Tissue Homogenates
    Expand to show all 17 Citations
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