Mouse TGF-beta  RII APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB532A-100
FAB532A-025
Mouse TGF-beta  RII APC-conjugated Antibody in Data
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Citations (5)
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Mouse TGF-beta  RII APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TGF-beta  RII in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) TGF-beta  RII is observed and less than 1% cross-reactivity with recombinant mouse TGF-beta  RI and rhTGF-beta  RIII is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TGF-beta  RII and S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF-beta  RII
Ile24-Asp184
Accession # Q62312
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TGF-beta  RII antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of TGF‑ beta RII in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse TGF-beta RII APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB532A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TGF-beta RII

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

The recombinant soluble TGF-beta Type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta Type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta.

References
  1. Miyazono, K. et al. (1994) Adv. in Immunol. 55:181.
Long Name
Transforming Growth Factor beta Receptor II
Entrez Gene IDs
7048 (Human); 21813 (Mouse)
Alternate Names
AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII; TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II

Product Datasheets

Citations for Mouse TGF-beta  RII APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Membrane-organizing protein moesin controls Treg differentiation and antitumor immunity via TGF-? signaling
    Authors: EA Ansa-Addo, Y Zhang, Y Yang, GS Hussey, BV Howley, M Salem, B Riesenberg, S Sun, DC Rockey, S Karvar, PH Howe, B Liu, Z Li
    J. Clin. Invest, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. MicroRNAs are implicated in the suppression of CD4+CD25- conventional T cell proliferation by CD4+CD25+ regulatory T cells.
    Authors: Zhou S, Dong X, Zhang C, Chen X, Zhu J, Li W, Song X, Xu Z, Zhang W, Yang X, Li Y, Liu F, Sun C
    Mol Immunol, 2015;63(2):464-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. TGF-beta3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses.
    Authors: Okamura T, Sumitomo S, Morita K, Iwasaki Y, Inoue M, Nakachi S, Komai T, Shoda H, Miyazaki J, Fujio K, Yamamoto K
    Nat Commun, 2015;6(0):6329.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Angiotensin II-dependent TGF-beta signaling contributes to Loeys-Dietz syndrome vascular pathogenesis.
    Authors: Gallo E, Loch D, Habashi J, Calderon J, Chen Y, Bedja D, van Erp C, Gerber E, Parker S, Sauls K, Judge D, Cooke S, Lindsay M, Rouf R, Myers L, ap Rhys C, Kent K, Norris R, Huso D, Dietz H
    J Clin Invest, 2014;124(1):448-60.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. PARP-1 regulates expression of TGF-beta receptors in T cells.
    Authors: Zhang P, Nakatsukasa H, Tu E, Kasagi S, Cui K, Ishikawa M, Konkel J, Maruyama T, Wei G, Abbatiello B, Wang Z, Zhao K, Chen W
    Blood, 0;122(13):2224-32.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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