Mouse TGF-beta 2 Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TGF‑ beta 2 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) TGF-beta 2 and rhTGF-beta 3 is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 771213
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse TGF‑ beta 2
    Ala303-Ser414
    Accession # P27090
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize TGF‑ beta 2 inhibition of IL-4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 0.15-0.75 ug/mL in the presence of 5 ng/mL Recombinant Mouse TGF‑ beta 2 and 7.5 ng/mL Recombinant Mouse IL-4.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
TGF‑ beta 2 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by Mouse TGF‑ beta 2 Antibody. Recombinant Mouse TGF‑ beta 2 (Catalog # 7346‑B2) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Mouse TGF-beta 2 (5 ng/mL) is neutralized (green line) by increasing concen­trations of Rat Anti-Mouse TGF‑ beta 2 Monoclonal Antibody (Catalog # MAB7346). The ND50 is typically 0.15-0.75 ug/mL.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta 2
TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta  superfamily that share a characteristic cysteine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems. Mouse TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 283 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-  beta 2. Disulfide-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 2 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature mouse TGF-beta 2 shares 100% aa identity with rat TGF-beta 2, and 97% aa identity with human, porcine, canine, equine and bovine TGF-beta 2. It demonstrates cross-species activity. In most cells, TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta  RIII) and a type II ser/thr kinase receptor termed TGF-beta  RII, which then phosphorylates and activates another ser/thr kinase receptor, TGF-beta  RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and activates Smad proteins that regulate transcription. In bone -related cells, however, TGF-beta 2 also signals through TGF-beta RIIB (a splice variant of TGF-beta RII), independently of TGF-beta RIII. Use of other signaling pathways that are Smad-independent allows for disparate actions observed in response to TGF-beta in different contexts.
  • Long Name:
    Transforming Growth Factor beta 2
  • Entrez Gene IDs:
    7042 (Human); 21808 (Mouse); 397084 (Porcine)
  • Alternate Names:
    BSC-1 cell growth inhibitor; cetermin; Glioblastoma-derived T-cell suppressor factor; G-TSF; MGC116892; polyergin; TGFB2; TGFbeta 2; TGF-beta 2; TGF-beta2; TGF-beta-2; transforming growth factor beta-2; transforming growth factor, beta 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. CYP4A in tumor-associated macrophages promotes pre-metastatic niche formation and metastasis
    Authors: XW Chen, TJ Yu, J Zhang, Y Li, HL Chen, GF Yang, W Yu, YZ Liu, XX Liu, CF Duan, HL Tang, M Qiu, CL Wang, H Zheng, J Yue, AM Guo, J Yang
    Oncogene, 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: Functional Assay
  2. Immunomodulatory cross-talk between conjunctival goblet cells and dendritic cells.
    Authors: Contreras-Ruiz L, Masli S
    PLoS ONE, 2015;10(3):e0120284.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: Neut
  3. Endothelial lineage differentiation from induced pluripotent stem cells is regulated by microRNA-21 and transforming growth factor beta2 (TGF-beta2) pathways.
    Authors: Di Bernardini E, Campagnolo P, Margariti A, Zampetaki A, Karamariti E, Hu Y, Xu Q
    J Biol Chem, 2013;289(6):3383-93.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
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Rat IgG2B Isotype Control

Ctrl MAB0061 27  
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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG2B Isotype Control

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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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