Mouse TGF-beta 2 Antibody
Mouse TGF-beta 2 Antibody Summary
Ala303-Ser414
Accession # P27090
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse TGF‑ beta 2 by Western Blot. Western blot shows lysates of Y-79 human retinoblastoma cell line, HUVEC human umbilical vein endothelial cells, NIH-3T3 mouse embryonic fibroblast cell line, 4T1 mouse breast cancer cell line, and NMuMG mouse mammary gland epithelial cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse TGF-beta 2 Monoclonal Antibody (Catalog # MAB73461) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TGF-beta 2 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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TGF‑ beta 2 in Mouse Embryo. TGF-beta 2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rat Anti-Mouse TGF-beta 2 Monoclonal Antibody (Catalog # MAB73461) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta 2
TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cysteine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems. Mouse TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 283 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 2. Disulfide-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 2 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature mouse TGF-beta 2 shares 100% aa identity with rat TGF-beta 2, and 97% aa identity with human, porcine, canine, equine and bovine TGF-beta 2. It demonstrates cross-species activity. In most cells, TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II ser/thr kinase receptor termed TGF-beta RII, which then phosphorylates and activates another ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and activates Smad proteins that regulate transcription. In bone -related cells, however, TGF-beta 2 also signals through TGF-beta RIIB (a splice variant of TGF-beta RII), independently of TGF-beta RIII. Use of other signaling pathways that are Smad-independent allows for disparate actions observed in response to TGF-beta in different contexts.
Product Datasheets
Citations for Mouse TGF-beta 2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Resveratrol protects mice against SEB-induced acute lung injury and mortality by miR-193a modulation that targets TGF-? signalling
Authors: H Alghetaa, A Mohammed, M Sultan, P Busbee, A Murphy, S Chatterjee, M Nagarkatti, P Nagarkatti
J. Cell. Mol. Med., 2018-03-07;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Targeting LOXL2 for cardiac interstitial fibrosis and heart failure treatment
Nat Commun, 2016-12-14;7(0):13710.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
TGF-?1 stimulates movement of renal proximal tubular epithelial cells in a three-dimensional cell culture via an autocrine TGF-?2 production
Authors: Deyi Luo
Exp. Cell Res, 2016-11-21;350(1):132-139.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Immunomodulatory cross-talk between conjunctival goblet cells and dendritic cells.
Authors: Contreras-Ruiz L, Masli S
PLoS ONE, 2015-03-20;10(3):e0120284.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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Reviews for Mouse TGF-beta 2 Antibody
Average Rating: 5 (Based on 1 Review)
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direct ELISA. Check the antibody did not cross react with rh TGF beta 1 protein because I wanted to use it as a detection antibody for a TGF beta 2 ELISA
