Detects mouse TGF‑ beta 2 in ELISAs and Western blots. In direct ELISAs, 100% cross‑reactivity with recombinant human (rh) TGF‑ beta 2, 25% cross‑reactivity with rhTGF‑ beta 3, and no cross‑reactivity with recombinant mouse TGF‑ beta 1 is observed.
Monoclonal Rat IgG2B Clone # 771244
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant mouse TGF-beta 2 Ala303-Ser414 Accession # P27090
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse TGF‑ beta 2 by Western Blot. Western blot shows lysates of Y‑79 human retinoblastoma cell line, HUVEC human umbilical vein endothelial cells, NIH‑3T3 mouse embryonic fibroblast cell line, 4T1 mouse breast cancer cell line, and NMuMG mouse mammary gland epithelial cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse TGF‑ beta 2 Monoclonal Antibody (Catalog # MAB73461) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TGF‑ beta 2 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TGF‑ beta 2 in Mouse Embryo. TGF‑ beta 2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rat Anti-Mouse TGF‑ beta 2 Monoclonal Antibody (Catalog # MAB73461) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta 2
(transforming growth factor beta 2) is one of three closely related mammalian
members of the large TGF-beta superfamily that share a characteristic cysteine
knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic
cytokines proposed to act as cellular switches that regulate processes
such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for
TGF-beta 2, mice with targeted deletion show defects in development of cardiac,
lung, craniofacial, limb, eye, ear and urogenital systems. Mouse TGF-beta 2
cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal
peptide and a 395 aa proprotein. A furin-like convertase processes the
proprotein to generate an N-terminal 283 aa latency-associated peptide (LAP)
and a C-terminal 112 aa mature TGF- beta 2. Disulfide-linked homodimers
of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the
small latent TGF-beta 2 complex. Covalent linkage of LAP to one of three
latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may
interact with the extracellular matrix. TGF-beta is activated from
latency by pathways that include actions of the protease plasmin, matrix
metalloproteases, thrombospondin 1 and a subset of integrins. Mature mouse
TGF-beta 2 shares 100% aa identity with rat TGF-beta 2, and 97% aa identity with
human, porcine, canine, equine and bovine TGF-beta 2. It demonstrates
cross-species activity. In most cells, TGF-beta 2 signaling begins with binding
to a complex of the accessory receptor betaglycan (also known as
TGF-beta RIII) and a type II ser/thr kinase receptor termed TGF-beta RII,
which then phosphorylates and activates another ser/thr kinase receptor,
TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or
alternatively, ALK-1. The whole complex phosphorylates and activates Smad
proteins that regulate transcription. In bone -related cells,
however, TGF-beta 2 also signals through TGF-beta RIIB (a splice variant of TGF-beta
RII), independently of TGF-beta RIII. Use of other signaling pathways
that are Smad-independent allows for disparate actions observed in response to
TGF-beta in different contexts.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.