Thrombospondin‑4 was detected in perfusion fixed frozen sections of mouse connective tissue using Rat Anti-Mouse Thrombospondin‑4 Monoclonal Antibody (Catalog # MAB7860) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse Thrombospondin-4 (TSP-4) is a 120-130 kDa adhesive glycoprotein that belongs to the thrombospondin family of proteins. It is 963 amino acids (aa) in length and highly modular in structure, containing one Lamnin G-like domain, four EGF-like domains and eight type 3 TSP domains. It circulates as a disulfide-linked homopentamer, and is up-regulated in the heart in response to ischemic injury and pathology. Mature mouse TSP-4 is 93% aa identical to human TSP4.
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