Mouse TIM-1/KIM-1/HAVCR Antibody
Mouse TIM-1/KIM-1/HAVCR Antibody Summary
Tyr22-Thr212
Accession # NP_001160104
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
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Detection of Mouse TIM‑1/KIM‑1/HAVCR by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1817) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TIM-1/KIM-1/HAVCR at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIM-1/KIM-1/HAVCR
TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVCR) is a 70-80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-4). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM genes (# 1-8) vs. only three genes in human (# 1, 3, and 4) (1, 2). Mouse TIM-1 and -2 are counterparts of human TIM-1 while mouse TIM-5 through 8 have no human counterparts (2). Mouse TIM-1 is synthesized as a 305 amino acid (aa) precursor that contains a 21 aa signal sequence, a 216 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (5, 6). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple T-S-P motifs. The mucin region undergoes extensive O-linked glycosylation. The mouse TIM-1 gene is highly polymorphic and, based on rat, may undergo alternate splicing (4, 6). For instance, HBA mice show a 15 aa deletion in the mucin region that occurs in BALB/c mice (6). This difference is associated with a decreased susceptibility to asthma. Other polymorphisms are also documented (6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing a 75-85 kDa soluble molecule (5). The same thing might be expected in mouse. The ECD of mouse TIM-1 is 50%, 39%, and 80% aa identical to human, canine and rat TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (8, 9). However, others are believed to exist, and based on the ligand for TIM-3, one possibility might be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10). In particular, it induces IL-4 production, and requires the cytoplasmic tyrosine phosphorylation motif (5).
- Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
- Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
- Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
- Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
- de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
- McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
- Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
- Feigelstock, D. et al. (1998) J. Virol. 72:6621.
- Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
- Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Product Datasheets
Citations for Mouse TIM-1/KIM-1/HAVCR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Long-term rescue of dystrophin expression and improvement in muscle pathology and function in dystrophic mdx mice by peptide-conjugated morpholino.
Am. J. Pathol., 2102;181(2):392-400.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Signaling pathways predisposing to chronic kidney disease progression
Authors: M Zaidan, M Burtin, JD Zhang, T Blanc, P Barre, S Garbay, C Nguyen, F Vasseur, L Yammine, S Germano, L Badi, MC Gubler, M Gallazzini, G Friedlande, M Pontoglio, F Terzi
JCI Insight, 2020;5(9):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
FOXM1 drives proximal tubule proliferation during repair from acute ischemic kidney injury
Authors: M Chang-Pane, FF Kadyrov, M Lalli, H Wu, S Ikeda, E Kefaloyian, MM Abdelmagee, A Herrlich, A Kobayashi, BD Humphreys
J. Clin. Invest., 2019;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Immunoglobulin light chains generate proinflammatory and profibrotic kidney injury
Authors: WZ Ying, X Li, S Rangarajan, W Feng, LM Curtis, PW Sanders
J. Clin. Invest., 2019;129(7):2792-2806.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Lack of Cathepsin D in the Renal Proximal Tubular Cells Resulted in Increased Sensitivity against Renal Ischemia/Reperfusion Injury
Authors: C Suzuki, I Tanida, M Ohmuraya, JA Oliva Trej, S Kakuta, T Sunabori, Y Uchiyama
Int J Mol Sci, 2019;20(7):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury
Authors: E Lazzeri, ML Angelotti, A Peired, C Conte, JA Marschner, L Maggi, B Mazzinghi, D Lombardi, ME Melica, S Nardi, E Ronconi, A Sisti, G Antonelli, F Becherucci, L De Chiara, RR Guevara, A Burger, B Schaefer, F Annunziato, HJ Anders, L Lasagni, P Romagnani
Nat Commun, 2018;9(1):1344.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Control release of mitochondria-targeted antioxidant by injectable self-assembling peptide hydrogel ameliorated persistent mitochondrial dysfunction and inflammation after acute kidney injury
Authors: M Zhao, Y Zhou, S Liu, L Li, Y Chen, J Cheng, Y Lu, J Liu
Drug Deliv, 2018;25(1):546-554.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy
Authors: O Maydan, PG McDade, Y Liu, XR Wu, DG Matsell, AA Eddy
Physiol Rep, 2018;6(6):e13654.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Forkhead Box O3 (FoxO3) Regulates Kidney Tubular Autophagy Following Urinary Tract Obstruction
Authors: L Li, R Zviti, C Ha, ZV Wang, JA Hill, F Lin
J. Biol. Chem., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Elevated urinary CRELD2 is associated with endoplasmic reticulum stress-mediated kidney disease
Authors: Y Kim, SJ Park, SR Manson, CA Molina, K Kidd, H Thiessen-P, RJ Perry, H Liapis, S Kmoch, CR Parikh, AJ Bleyer, YM Chen
JCI Insight, 2017;2(23):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
PINK1-PRKN/PARK2 pathway of mitophagy is activated to protect against Renal ischemia-reperfusion injury
Authors: C Tang, H Han, M Yan, S Zhu, J Liu, Z Liu, L He, J Tan, Y Liu, H Liu, L Sun, S Duan, Y Peng, F Liu, XM Yin, Z Zhang, Z Dong
Autophagy, 2017;0(0):1-45.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Molecular characterization of the transition from acute to chronic kidney injury following ischemia/reperfusion
Authors: J Liu, S Kumar, E Dolzhenko, GF Alvarado, J Guo, C Lu, Y Chen, M Li, MC Dessing, RK Parvez, PE Cippà, AM Krautzberg, G Saribekyan, AD Smith, AP McMahon
JCI Insight, 2017;2(18):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Kidney-specific Sonoporation-mediated Gene Transfer.
Authors: Ishida R, Kami D, Kusaba T, Kirita Y, Kishida T, Mazda O, Adachi T, Gojo S
Mol Ther, 2016;24(1):125-34.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Rescue therapy with Tanshinone IIA hinders transition of acute kidney injury to chronic kidney disease via targeting GSK3?
Sci Rep, 2016;6(0):36698.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Rapid Renal Regulation of Peroxisome Proliferator-activated Receptor ? Coactivator-1? by Extracellular Signal-Regulated Kinase 1/2 in Physiological and Pathological Conditions
Authors: JB Collier, RM Whitaker, ST Eblen, RG Schnellman
J. Biol. Chem., 2016;291(52):26850-26859.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Wnt4 is a novel biomarker for the early detection of kidney tubular injury after ischemia/reperfusion injury
Sci Rep, 2016;6(0):32610.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Characterizing functional domains for TIM-mediated enveloped virus entry.
Authors: Moller-Tank S, Albritton L, Rennert P, Maury W
J Virol, 2014;88(12):6702-13.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Repair of injured proximal tubule does not involve specialized progenitors.
Authors: Humphreys BD, Czerniak S, DiRocco DP
Proc. Natl. Acad. Sci. U.S.A., 2011;108(22):9226-31.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells.
Authors: Nakae S, Iikura M, Suto H, Akiba H, Umetsu DT, DeKruyff RH, Saito H, Galli SJ
Blood, 2007;110(7):2565-8.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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