Mouse TIM-1/KIM-1/HAVCR Antibody

AF1817 17 Citations Datasheet / COA / SDS

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AF1817
AF1817-SP

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Mouse TIM-1/KIM-1/HAVCR Antibody in Western Blot

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Citations (17)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TIM-1/KIM-1/HAVCR Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TIM‑1/KIM-1/HAVCR in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human TIM-1, recombinant mouse (rm) TIM-2, rmTIM-3, rmTIM-4, rmTIM-6, and rmTIM-7 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TIM‑1/KIM-1/HAVCR
Tyr22-Thr212
Accession # NP_001160104

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot

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Detection of Mouse TIM‑1/KIM‑1/HAVCR by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1817) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TIM-1/KIM-1/HAVCR at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-1/KIM-1/HAVCR

TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVCR) is a 70-80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-4). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM genes (# 1-8) vs. only three genes in human (# 1, 3, and 4) (1, 2). Mouse TIM-1 and -2 are counterparts of human TIM-1 while mouse TIM-5 through 8 have no human counterparts (2). Mouse TIM-1 is synthesized as a 305 amino acid (aa) precursor that contains a 21 aa signal sequence, a 216 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (5, 6). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple T-S-P motifs. The mucin region undergoes extensive O-linked glycosylation. The mouse TIM-1 gene is highly polymorphic and, based on rat, may undergo alternate splicing (4, 6). For instance, HBA mice show a 15 aa deletion in the mucin region that occurs in BALB/c mice (6). This difference is associated with a decreased susceptibility to asthma. Other polymorphisms are also documented (6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing a 75-85 kDa soluble molecule (5). The same thing might be expected in mouse. The ECD of mouse TIM-1 is 50%, 39%, and 80% aa identical to human, canine and rat TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (8, 9). However, others are believed to exist, and based on the ligand for TIM-3, one possibility might be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10). In particular, it induces IL-4 production, and requires the cytoplasmic tyrosine phosphorylation motif (5).

References

Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
Feigelstock, D. et al. (1998) J. Virol. 72:6621.
Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.

Long Name

T Cell Immunoglobulin Mucin-1

Entrez Gene IDs

26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)

Alternate Names

CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1

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SDS

Related Research Areas

Citations for Mouse TIM-1/KIM-1/HAVCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Long-term rescue of dystrophin expression and improvement in muscle pathology and function in dystrophic mdx mice by peptide-conjugated morpholino.
Am. J. Pathol., 2102;181(2):392-400.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Immunoglobulin light chains generate proinflammatory and profibrotic kidney injury
Authors: WZ Ying, X Li, S Rangarajan, W Feng, LM Curtis, PW Sanders
J. Clin. Invest., 2019;129(7):2792-2806.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Lack of Cathepsin D in the Renal Proximal Tubular Cells Resulted in Increased Sensitivity against Renal Ischemia/Reperfusion Injury
Authors: C Suzuki, I Tanida, M Ohmuraya, JA Oliva Trej, S Kakuta, T Sunabori, Y Uchiyama
Int J Mol Sci, 2019;20(7):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury
Authors: E Lazzeri, ML Angelotti, A Peired, C Conte, JA Marschner, L Maggi, B Mazzinghi, D Lombardi, ME Melica, S Nardi, E Ronconi, A Sisti, G Antonelli, F Becherucci, L De Chiara, RR Guevara, A Burger, B Schaefer, F Annunziato, HJ Anders, L Lasagni, P Romagnani
Nat Commun, 2018;9(1):1344.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Control release of mitochondria-targeted antioxidant by injectable self-assembling peptide hydrogel ameliorated persistent mitochondrial dysfunction and inflammation after acute kidney injury
Authors: M Zhao, Y Zhou, S Liu, L Li, Y Chen, J Cheng, Y Lu, J Liu
Drug Deliv, 2018;25(1):546-554.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy
Authors: O Maydan, PG McDade, Y Liu, XR Wu, DG Matsell, AA Eddy
Physiol Rep, 2018;6(6):e13654.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Elevated urinary CRELD2 is associated with endoplasmic reticulum stress-mediated kidney disease
Authors: Y Kim, SJ Park, SR Manson, CA Molina, K Kidd, H Thiessen-P, RJ Perry, H Liapis, S Kmoch, CR Parikh, AJ Bleyer, YM Chen
JCI Insight, 2017;2(23):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
PINK1-PRKN/PARK2 pathway of mitophagy is activated to protect against Renal ischemia-reperfusion injury
Authors: C Tang, H Han, M Yan, S Zhu, J Liu, Z Liu, L He, J Tan, Y Liu, H Liu, L Sun, S Duan, Y Peng, F Liu, XM Yin, Z Zhang, Z Dong
Autophagy, 2017;0(0):1-45.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Molecular characterization of the transition from acute to chronic kidney injury following ischemia/reperfusion
Authors: J Liu, S Kumar, E Dolzhenko, GF Alvarado, J Guo, C Lu, Y Chen, M Li, MC Dessing, RK Parvez, PE Cippà, AM Krautzberg, G Saribekyan, AD Smith, AP McMahon
JCI Insight, 2017;2(18):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Forkhead Box O3 (FoxO3) Regulates Kidney Tubular Autophagy Following Urinary Tract Obstruction
Authors: L Li, R Zviti, C Ha, ZV Wang, JA Hill, F Lin
J. Biol. Chem., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Rescue therapy with Tanshinone IIA hinders transition of acute kidney injury to chronic kidney disease via targeting GSK3?
Sci Rep, 2016;6(0):36698.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Kidney-specific Sonoporation-mediated Gene Transfer.
Authors: Ishida R, Kami D, Kusaba T, Kirita Y, Kishida T, Mazda O, Adachi T, Gojo S
Mol Ther, 2016;24(1):125-34.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Wnt4 is a novel biomarker for the early detection of kidney tubular injury after ischemia/reperfusion injury
Sci Rep, 2016;6(0):32610.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Rapid Renal Regulation of Peroxisome Proliferator-activated Receptor ? Coactivator-1? by Extracellular Signal-Regulated Kinase 1/2 in Physiological and Pathological Conditions
Authors: JB Collier, RM Whitaker, ST Eblen, RG Schnellman
J. Biol. Chem., 2016;291(52):26850-26859.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Characterizing functional domains for TIM-mediated enveloped virus entry.
Authors: Moller-Tank S, Albritton L, Rennert P, Maury W
J Virol, 2014;88(12):6702-13.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Repair of injured proximal tubule does not involve specialized progenitors.
Authors: Humphreys BD, Czerniak S, DiRocco DP
Proc. Natl. Acad. Sci. U.S.A., 2011;108(22):9226-31.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells.
Authors: Nakae S, Iikura M, Suto H, Akiba H, Umetsu DT, DeKruyff RH, Saito H, Galli SJ
Blood, 2007;110(7):2565-8.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot