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Mouse TIM-1/KIM-1/HAVCR Antibody

Catalog #: AF1817
39 Citations 1 Review Datasheet / COA / SDS
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AF1817
AF1817-SP
Detection of Mouse TIM‑1/KIM‑1/HAVCR by Western Blot.
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Citations (39)
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Mouse TIM-1/KIM-1/HAVCR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TIM‑1/KIM-1/HAVCR in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human TIM-1, recombinant mouse (rm) TIM-2, rmTIM-3, rmTIM-4, rmTIM-6, and rmTIM-7 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TIM‑1/KIM-1/HAVCR
Tyr22-Thr212
Accession # NP_001160104
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse TIM‑1/KIM‑1/HAVCR antibody by Western Blot. View Larger

Detection of Mouse TIM‑1/KIM‑1/HAVCR by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1817) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TIM-1/KIM-1/HAVCR at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-1/KIM-1/HAVCR

TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVCR) is a 70-80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-4). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM genes (# 1-8) vs. only three genes in human (# 1, 3, and 4) (1, 2). Mouse TIM-1 and -2 are counterparts of human TIM-1 while mouse TIM-5 through 8 have no human counterparts (2). Mouse TIM-1 is synthesized as a 305 amino acid (aa) precursor that contains a 21 aa signal sequence, a 216 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (5, 6). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple T-S-P motifs. The mucin region undergoes extensive O-linked glycosylation. The mouse TIM-1 gene is highly polymorphic and, based on rat, may undergo alternate splicing (4, 6). For instance, HBA mice show a 15 aa deletion in the mucin region that occurs in BALB/c mice (6). This difference is associated with a decreased susceptibility to asthma. Other polymorphisms are also documented (6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing a 75-85 kDa soluble molecule (5). The same thing might be expected in mouse. The ECD of mouse TIM-1 is 50%, 39%, and 80% aa identical to human, canine and rat TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (8, 9). However, others are believed to exist, and based on the ligand for TIM-3, one possibility might be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10). In particular, it induces IL-4 production, and requires the cytoplasmic tyrosine phosphorylation motif (5).

References
  1. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471. 
  2. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
  3. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.  
  4. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.  
  5. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113. 
  6. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  7. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
  8. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  9. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  10. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Long Name
T Cell Immunoglobulin Mucin-1
Entrez Gene IDs
26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
Alternate Names
CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1

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Citations for Mouse TIM-1/KIM-1/HAVCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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  1. Platelet-instructed SPP1+ macrophages drive myofibroblast activation in fibrosis in a CXCL4-dependent manner
    Authors: K Hoeft, GJL Schaefer, H Kim, D Schumacher, T Bleckwehl, Q Long, BM Klinkhamme, F Peisker, L Koch, J Nagai, M Halder, S Ziegler, E Liehn, C Kuppe, J Kranz, S Menzel, I Costa, A Wahida, P Boor, RK Schneider, S Hayat, R Kramann
    Cell Reports, 2023-02-17;42(2):112131.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Cytoplasmic HMGB1 induces renal tubular ferroptosis after ischemia/reperfusion
    Authors: Z Zhao, G Li, Y Wang, Y Li, H Xu, W Liu, W Hao, Y Yao, R Zeng
    International immunopharmacology, 2023-02-01;116(0):109757.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Chromatin accessibility dynamics dictate renal tubular epithelial cell response to injury
    Authors: X Cao, J Wang, T Zhang, Z Liu, L Liu, Y Chen, Z Li, Y Zhao, Q Yu, T Liu, J Nie, Y Niu, Y Chen, L Yang, L Zhang
    Nature Communications, 2022-11-28;13(1):7322.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Sex differences in resilience to ferroptosis underlie sexual dimorphism in kidney injury and repair
    Authors: S Ide, K Ide, K Abe, Y Kobayashi, H Kitai, J McKey, SA Strausser, LL O'Brien, A Tata, PR Tata, T Souma
    Cell Reports, 2022-11-08;41(6):111610.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Nephroprotective Effects of Semaglutide as Mono- and Combination Treatment with Lisinopril in a Mouse Model of Hypertension-Accelerated Diabetic Kidney Disease
    Authors: LS Dalbøge, M Christense, MR Madsen, T Secher, N Endlich, V Drenic', A Manresa-Ar, HH Hansen, I Rune, LN Fink, MV Østergaard
    Biomedicines, 2022-07-11;10(7):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Tubular cells produce FGF2 via autophagy after acute kidney injury leading to fibroblast activation and renal fibrosis
    Authors: MJ Livingston, S Shu, Y Fan, Z Li, Q Jiao, XM Yin, MA Venkatacha, Z Dong
    Autophagy, 2022-05-18;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Sphingosine 1-Phosphate Receptor 5 (S1P5) Knockout Ameliorates Adenine-Induced Nephropathy
    Authors: T Eckes, S Patyna, A Koch, A Oftring, S Gauer, N Obermüller, S Schwalm, L Schaefer, J Chun, HJ Gröne, J Pfeilschif
    International Journal of Molecular Sciences, 2022-04-02;23(7):.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  8. Snapshots of nascent RNA reveal cell- and stimulus- specific responses to acute kidney injury
    Authors: TH Shen, J Stauber, K Xu, A Jacunski, N Paragas, M Callahan, R Banlengchi, AD Levitman, B Desanti de, A Beenken, MS Grau, E Mathieu, Q Zhang, Y Li, T Gopal, N Askanase, S Arumugam, S Mohan, PI Good, JS Stevens, F Lin, SK Sia, CS Lin, V D'Agati, K Kiryluk, NP Tatonetti, J Barasch
    JCI Insight, 2022-03-22;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Hepatitis A Virus Cellular Receptor 1 (HAVcr-1) Initiates Prostate Cancer Progression in Human Cells via Hepatocyte Growth Factor (HGF)-Induced Changes in Junctional Integrity
    Authors: EA Telford, AJ Sanders, S Owen, F Ruge, GM Harrison, WG Jiang, TA Martin
    Biomolecules, 2022-02-21;12(2):.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  10. Activation of Transcription Factor EB Alleviates Tubular Epithelial Cell Injury via Restoring Lysosomal Homeostasis in Diabetic Nephropathy
    Authors: S Wang, K Jing, H Wu, X Li, C Yang, T Li, H Tang, T Zou, Y She, HF Liu
    Oxidative Medicine and Cellular Longevity, 2022-01-12;2022(0):2812493.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Hydroxychloroquine administration exacerbates acute kidney injury complicated by lupus nephritis
    Authors: N An, C Yang, HL Wu, Y Guo, XJ Huang, TS Huang, ZH Wu, J Xue, RH Chen, ZH Li, QJ Pan, HF Liu
    Arthritis Research & Therapy, 2022-01-03;24(1):6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Loss of renal olfactory receptor 1393 leads to improved glucose homeostasis in a type 1 diabetic mouse model
    Authors: AR Schiazza, EG Considine, M Betcher, BD Shepard
    Physiological Reports, 2021-12-01;9(23):e15007.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Aldehyde-driven transcriptional stress triggers an anorexic DNA damage response
    Authors: L Mulderrig, JI Garaycoech, ZK Tuong, CL Millington, FA Dingler, JR Ferdinand, L Gaul, JA Tadross, MJ Arends, S O'Rahilly, GP Crossan, MR Clatworthy, KJ Patel
    Nature, 2021-11-24;600(7887):158-163.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Farrerol Ameliorated Cisplatin-Induced Chronic Kidney Disease Through Mitophagy Induction via Nrf2/PINK1 Pathway
    Authors: N Ma, Z Wei, J Hu, W Gu, X Ci
    Frontiers in Pharmacology, 2021-11-11;12(0):768700.
    Species: Mouse, Transgenic Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  15. Deletion of Smad3 protects against C-reactive protein-induced renal fibrosis and inflammation in obstructive nephropathy
    Authors: YK You, WF Wu, XR Huang, HD Li, YP Ren, JC Zeng, H Chen, HY Lan
    International journal of biological sciences, 2021-09-21;17(14):3911-3922.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Kidney injury molecule-1 inhibits metastasis of renal cell carcinoma
    Authors: JC Lee, DM Yotis, JY Lee, MA Sarabusky, B Shrum, A Champagne, OZ Ismail, E Tutunea-Fa, HS Leong, L Gunaratnam
    Scientific Reports, 2021-06-04;11(1):11840.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Combination therapy of cisplatin with cilastatin enables an increased dose of cisplatin, enhancing its antitumor effect by suppression of nephrotoxicity
    Authors: M Arita, S Watanabe, N Aoki, S Kuwahara, R Suzuki, S Goto, Y Abe, M Takahashi, M Sato, S Hokari, A Ohtsubo, S Shoji, K Nozaki, K Ichikawa, R Kondo, M Hayashi, Y Ohshima, H Kabasawa, M Hosojima, T Koya, A Saito, T Kikuchi
    Scientific Reports, 2021-01-12;11(1):750.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. The sodium-glucose cotransporter 2 inhibitor tofogliflozin prevents diabetic kidney disease progression in type 2 diabetic mice
    Authors: Z Li, M Murakoshi, S Ichikawa, T Koshida, E Adachi, C Suzuki, S Ueda, T Gohda, Y Suzuki
    FEBS Open Bio, 2020-11-10;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Vagus nerve stimulation even after injury ameliorates cisplatin-induced nephropathy via reducing macrophage infiltration
    Authors: R Uni, T Inoue, Y Nakamura, D Fukaya, S Hasegawa, CH Wu, R Fujii, B Suratticha, W Peerapanya, A Ozeki, N Akimitsu, Y Wada, M Nangaku, R Inagi
    Sci Rep, 2020-06-11;10(1):9472.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Signaling pathways predisposing to chronic kidney disease progression
    Authors: M Zaidan, M Burtin, JD Zhang, T Blanc, P Barre, S Garbay, C Nguyen, F Vasseur, L Yammine, S Germano, L Badi, MC Gubler, M Gallazzini, G Friedlande, M Pontoglio, F Terzi
    JCI Insight, 2020-05-07;5(9):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Phenformin Attenuates Renal Injury in Unilateral Ureteral Obstructed Mice without Affecting Immune Cell Infiltration
    Authors: MØ Nørgård, M Christense, H A M Mutsae, R Nørregaard
    Pharmaceutics, 2020-03-26;12(4):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. FOXM1 drives proximal tubule proliferation during repair from acute ischemic kidney injury
    Authors: M Chang-Pane, FF Kadyrov, M Lalli, H Wu, S Ikeda, E Kefaloyian, MM Abdelmagee, A Herrlich, A Kobayashi, BD Humphreys
    J. Clin. Invest., 2019-12-02;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  23. Immunoglobulin light chains generate proinflammatory and profibrotic kidney injury
    Authors: WZ Ying, X Li, S Rangarajan, W Feng, LM Curtis, PW Sanders
    J. Clin. Invest., 2019-06-17;129(7):2792-2806.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. Lack of Cathepsin D in the Renal Proximal Tubular Cells Resulted in Increased Sensitivity against Renal Ischemia/Reperfusion Injury
    Authors: C Suzuki, I Tanida, M Ohmuraya, JA Oliva Trej, S Kakuta, T Sunabori, Y Uchiyama
    Int J Mol Sci, 2019-04-05;20(7):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  25. Control release of mitochondria-targeted antioxidant by injectable self-assembling peptide hydrogel ameliorated persistent mitochondrial dysfunction and inflammation after acute kidney injury
    Authors: M Zhao, Y Zhou, S Liu, L Li, Y Chen, J Cheng, Y Lu, J Liu
    Drug Deliv, 2018-11-01;25(1):546-554.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  26. Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury
    Authors: E Lazzeri, ML Angelotti, A Peired, C Conte, JA Marschner, L Maggi, B Mazzinghi, D Lombardi, ME Melica, S Nardi, E Ronconi, A Sisti, G Antonelli, F Becherucci, L De Chiara, RR Guevara, A Burger, B Schaefer, F Annunziato, HJ Anders, L Lasagni, P Romagnani
    Nat Commun, 2018-04-09;9(1):1344.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  27. Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy
    Authors: O Maydan, PG McDade, Y Liu, XR Wu, DG Matsell, AA Eddy
    Physiol Rep, 2018-03-01;6(6):e13654.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  28. PINK1-PRKN/PARK2 pathway of mitophagy is activated to protect against Renal ischemia-reperfusion injury
    Authors: C Tang, H Han, M Yan, S Zhu, J Liu, Z Liu, L He, J Tan, Y Liu, H Liu, L Sun, S Duan, Y Peng, F Liu, XM Yin, Z Zhang, Z Dong
    Autophagy, 2018-02-17;0(0):1-45.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  29. Elevated urinary CRELD2 is associated with endoplasmic reticulum stress-mediated kidney disease
    Authors: Y Kim, SJ Park, SR Manson, CA Molina, K Kidd, H Thiessen-P, RJ Perry, H Liapis, S Kmoch, CR Parikh, AJ Bleyer, YM Chen
    JCI Insight, 2017-12-07;2(23):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  30. Molecular characterization of the transition from acute to chronic kidney injury following ischemia/reperfusion
    Authors: J Liu, S Kumar, E Dolzhenko, GF Alvarado, J Guo, C Lu, Y Chen, M Li, MC Dessing, RK Parvez, PE Cippà, AM Krautzberg, G Saribekyan, AD Smith, AP McMahon
    JCI Insight, 2017-09-21;2(18):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  31. Forkhead Box O3 (FoxO3) Regulates Kidney Tubular Autophagy Following Urinary Tract Obstruction
    Authors: L Li, R Zviti, C Ha, ZV Wang, JA Hill, F Lin
    J. Biol. Chem., 2017-07-13;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  32. Rescue therapy with Tanshinone IIA hinders transition of acute kidney injury to chronic kidney disease via targeting GSK3?
    Sci Rep, 2016-11-18;6(0):36698.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  33. Rapid Renal Regulation of Peroxisome Proliferator-activated Receptor ? Coactivator-1? by Extracellular Signal-Regulated Kinase 1/2 in Physiological and Pathological Conditions
    Authors: JB Collier, RM Whitaker, ST Eblen, RG Schnellman
    J. Biol. Chem., 2016-11-14;291(52):26850-26859.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  34. Wnt4 is a novel biomarker for the early detection of kidney tubular injury after ischemia/reperfusion injury
    Sci Rep, 2016-09-07;6(0):32610.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  35. Kidney-specific Sonoporation-mediated Gene Transfer.
    Authors: Ishida R, Kami D, Kusaba T, Kirita Y, Kishida T, Mazda O, Adachi T, Gojo S
    Mol Ther, 2015-09-30;24(1):125-34.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  36. Characterizing functional domains for TIM-mediated enveloped virus entry.
    Authors: Moller-Tank S, Albritton L, Rennert P, Maury W
    J Virol, 2014-04-02;88(12):6702-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  37. Long-term rescue of dystrophin expression and improvement in muscle pathology and function in dystrophic mdx mice by peptide-conjugated morpholino.
    Am. J. Pathol., 2012-06-07;181(2):392-400.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  38. Repair of injured proximal tubule does not involve specialized progenitors.
    Authors: Humphreys BD, Czerniak S, DiRocco DP
    Proc. Natl. Acad. Sci. U.S.A., 2011-05-16;108(22):9226-31.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  39. TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells.
    Authors: Nakae S, Iikura M, Suto H, Akiba H, Umetsu DT, DeKruyff RH, Saito H, Galli SJ
    Blood, 2007-07-09;110(7):2565-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Mouse TIM-1/KIM-1/HAVCR Antibody
By Anonymous on 12/02/2016
Application: WB Sample Tested: Kidney tissue (cortex) Species: Mouse
Western Blot Mouse TIM-1/KIM-1/HAVCR Antibody AF1817