Mouse TIM-1/KIM-1/HAVCR Antibody

Catalog # Availability Size / Price Qty
AF1817
AF1817-SP
Detection of Mouse TIM‑1/KIM‑1/HAVCR by Western Blot.
1 Image
Product Details
Citations (14)
FAQs
Supplemental Products
Reviews (1)

Mouse TIM-1/KIM-1/HAVCR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TIM‑1/KIM-1/HAVCR in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human TIM-1, recombinant mouse (rm) TIM-2, rmTIM-3, rmTIM-4, rmTIM-6, and rmTIM-7 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TIM‑1/KIM-1/HAVCR
Tyr22-Thr212
Accession # NP_001160104
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse TIM‑1/KIM‑1/HAVCR by Western Blot. View Larger

Detection of Mouse TIM‑1/KIM‑1/HAVCR by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse TIM‑1/KIM‑1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1817) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TIM‑1/KIM‑1/HAVCR at approximately
70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-1/KIM-1/HAVCR

TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVCR) is a 70-80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-4). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM genes (# 1-8) vs. only three genes in human (# 1, 3, and 4) (1, 2). Mouse TIM-1 and -2 are counterparts of human TIM-1 while mouse TIM-5 through 8 have no human counterparts (2). Mouse TIM-1 is synthesized as a 305 amino acid (aa) precursor that contains a 21 aa signal sequence, a 216 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (5, 6). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple T-S-P motifs. The mucin region undergoes extensive O-linked glycosylation. The mouse TIM-1 gene is highly polymorphic and, based on rat, may undergo alternate splicing (4, 6). For instance, HBA mice show a 15 aa deletion in the mucin region that occurs in BALB/c mice (6). This difference is associated with a decreased susceptibility to asthma. Other polymorphisms are also documented (6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing a 75-85 kDa soluble molecule (5). The same thing might be expected in mouse. The ECD of mouse TIM-1 is 50%, 39%, and 80% aa identical to human, canine and rat TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (8, 9). However, others are believed to exist, and based on the ligand for TIM-3, one possibility might be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10). In particular, it induces IL-4 production, and requires the cytoplasmic tyrosine phosphorylation motif (5).

References
  1. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471. 
  2. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
  3. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.  
  4. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.  
  5. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113. 
  6. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  7. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
  8. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  9. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  10. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Long Name
T Cell Immunoglobulin Mucin-1
Entrez Gene IDs
26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
Alternate Names
CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1

Product Datasheets

Citations for Mouse TIM-1/KIM-1/HAVCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Long-term rescue of dystrophin expression and improvement in muscle pathology and function in dystrophic mdx mice by peptide-conjugated morpholino.
    Am. J. Pathol., 2102;181(2):392-400.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  2. Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy
    Authors: O Maydan, PG McDade, Y Liu, XR Wu, DG Matsell, AA Eddy
    Physiol Rep, 2018;6(6):e13654.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  3. Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury
    Authors: E Lazzeri, ML Angelotti, A Peired, C Conte, JA Marschner, L Maggi, B Mazzinghi, D Lombardi, ME Melica, S Nardi, E Ronconi, A Sisti, G Antonelli, F Becherucci, L De Chiara, RR Guevara, A Burger, B Schaefer, F Annunziato, HJ Anders, L Lasagni, P Romagnani
    Nat Commun, 2018;9(1):1344.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Elevated urinary CRELD2 is associated with endoplasmic reticulum stress-mediated kidney disease
    Authors: Y Kim, SJ Park, SR Manson, CA Molina, K Kidd, H Thiessen-P, RJ Perry, H Liapis, S Kmoch, CR Parikh, AJ Bleyer, YM Chen
    JCI Insight, 2017;2(23):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  5. PINK1-PRKN/PARK2 pathway of mitophagy is activated to protect against Renal ischemia-reperfusion injury
    Authors: C Tang, H Han, M Yan, S Zhu, J Liu, Z Liu, L He, J Tan, Y Liu, H Liu, L Sun, S Duan, Y Peng, F Liu, XM Yin, Z Zhang, Z Dong
    Autophagy, 2017;0(0):1-45.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  6. Molecular characterization of the transition from acute to chronic kidney injury following ischemia/reperfusion
    Authors: J Liu, S Kumar, E Dolzhenko, GF Alvarado, J Guo, C Lu, Y Chen, M Li, MC Dessing, RK Parvez, PE Cippà, AM Krautzberg, G Saribekyan, AD Smith, AP McMahon
    JCI Insight, 2017;2(18):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Forkhead Box O3 (FoxO3) Regulates Kidney Tubular Autophagy Following Urinary Tract Obstruction
    Authors: L Li, R Zviti, C Ha, ZV Wang, JA Hill, F Lin
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Kidney-specific Sonoporation-mediated Gene Transfer.
    Authors: Ishida R, Kami D, Kusaba T, Kirita Y, Kishida T, Mazda O, Adachi T, Gojo S
    Mol Ther, 2016;24(1):125-34.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  9. Rapid Renal Regulation of Peroxisome Proliferator-activated Receptor ? Coactivator-1? by Extracellular Signal-Regulated Kinase 1/2 in Physiological and Pathological Conditions
    Authors: JB Collier, RM Whitaker, ST Eblen, RG Schnellman
    J. Biol. Chem., 2016;291(52):26850-26859.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  10. Wnt4 is a novel biomarker for the early detection of kidney tubular injury after ischemia/reperfusion injury
    Sci Rep, 2016;6(0):32610.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  11. Rescue therapy with Tanshinone IIA hinders transition of acute kidney injury to chronic kidney disease via targeting GSK3?
    Sci Rep, 2016;6(0):36698.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  12. Characterizing functional domains for TIM-mediated enveloped virus entry.
    Authors: Moller-Tank S, Albritton L, Rennert P, Maury W
    J Virol, 2014;88(12):6702-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  13. Repair of injured proximal tubule does not involve specialized progenitors.
    Authors: Humphreys BD, Czerniak S, DiRocco DP
    Proc. Natl. Acad. Sci. U.S.A., 2011;108(22):9226-31.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  14. TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells.
    Authors: Nakae S, Iikura M, Suto H, Akiba H, Umetsu DT, DeKruyff RH, Saito H, Galli SJ
    Blood, 2007;110(7):2565-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse TIM-1/KIM-1/HAVCR Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Mouse TIM-1/KIM-1/HAVCR Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse TIM-1/KIM-1/HAVCR Antibody
By Anonymous on 12/02/2016
Application: WB Sample Tested: Kidney tissue (cortex) Species: Mouse