Mouse TIM-1/KIM-1/HAVCR Antibody Summary
Tyr22-Thr212
Accession # NP_001160104
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data

Detection of Mouse TIM‑1/KIM‑1/HAVCR by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1817) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TIM-1/KIM-1/HAVCR at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIM-1/KIM-1/HAVCR
TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVCR) is a 70-80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-4). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM genes (# 1-8) vs. only three genes in human (# 1, 3, and 4) (1, 2). Mouse TIM-1 and -2 are counterparts of human TIM-1 while mouse TIM-5 through 8 have no human counterparts (2). Mouse TIM-1 is synthesized as a 305 amino acid (aa) precursor that contains a 21 aa signal sequence, a 216 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (5, 6). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple T-S-P motifs. The mucin region undergoes extensive O-linked glycosylation. The mouse TIM-1 gene is highly polymorphic and, based on rat, may undergo alternate splicing (4, 6). For instance, HBA mice show a 15 aa deletion in the mucin region that occurs in BALB/c mice (6). This difference is associated with a decreased susceptibility to asthma. Other polymorphisms are also documented (6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing a 75-85 kDa soluble molecule (5). The same thing might be expected in mouse. The ECD of mouse TIM-1 is 50%, 39%, and 80% aa identical to human, canine and rat TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (8, 9). However, others are believed to exist, and based on the ligand for TIM-3, one possibility might be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10). In particular, it induces IL-4 production, and requires the cytoplasmic tyrosine phosphorylation motif (5).
- Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
- Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
- Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
- Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
- de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
- McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
- Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
- Feigelstock, D. et al. (1998) J. Virol. 72:6621.
- Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
- Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Product Datasheets
Citations for Mouse TIM-1/KIM-1/HAVCR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Platelet-instructed SPP1+ macrophages drive myofibroblast activation in fibrosis in a CXCL4-dependent manner
Authors: K Hoeft, GJL Schaefer, H Kim, D Schumacher, T Bleckwehl, Q Long, BM Klinkhamme, F Peisker, L Koch, J Nagai, M Halder, S Ziegler, E Liehn, C Kuppe, J Kranz, S Menzel, I Costa, A Wahida, P Boor, RK Schneider, S Hayat, R Kramann
Cell Reports, 2023-02-17;42(2):112131.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Cytoplasmic HMGB1 induces renal tubular ferroptosis after ischemia/reperfusion
Authors: Z Zhao, G Li, Y Wang, Y Li, H Xu, W Liu, W Hao, Y Yao, R Zeng
International immunopharmacology, 2023-02-01;116(0):109757.
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC -
Chromatin accessibility dynamics dictate renal tubular epithelial cell response to injury
Authors: X Cao, J Wang, T Zhang, Z Liu, L Liu, Y Chen, Z Li, Y Zhao, Q Yu, T Liu, J Nie, Y Niu, Y Chen, L Yang, L Zhang
Nature Communications, 2022-11-28;13(1):7322.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Sex differences in resilience to ferroptosis underlie sexual dimorphism in kidney injury and repair
Authors: S Ide, K Ide, K Abe, Y Kobayashi, H Kitai, J McKey, SA Strausser, LL O'Brien, A Tata, PR Tata, T Souma
Cell Reports, 2022-11-08;41(6):111610.
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC -
Nephroprotective Effects of Semaglutide as Mono- and Combination Treatment with Lisinopril in a Mouse Model of Hypertension-Accelerated Diabetic Kidney Disease
Authors: LS Dalbøge, M Christense, MR Madsen, T Secher, N Endlich, V Drenic', A Manresa-Ar, HH Hansen, I Rune, LN Fink, MV Østergaard
Biomedicines, 2022-07-11;10(7):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Tubular cells produce FGF2 via autophagy after acute kidney injury leading to fibroblast activation and renal fibrosis
Authors: MJ Livingston, S Shu, Y Fan, Z Li, Q Jiao, XM Yin, MA Venkatacha, Z Dong
Autophagy, 2022-05-18;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Sphingosine 1-Phosphate Receptor 5 (S1P5) Knockout Ameliorates Adenine-Induced Nephropathy
Authors: T Eckes, S Patyna, A Koch, A Oftring, S Gauer, N Obermüller, S Schwalm, L Schaefer, J Chun, HJ Gröne, J Pfeilschif
International Journal of Molecular Sciences, 2022-04-02;23(7):.
Species: Mouse
Sample Types: Tissue Lysates
Applications: Western Blot -
Snapshots of nascent RNA reveal cell- and stimulus- specific responses to acute kidney injury
Authors: TH Shen, J Stauber, K Xu, A Jacunski, N Paragas, M Callahan, R Banlengchi, AD Levitman, B Desanti de, A Beenken, MS Grau, E Mathieu, Q Zhang, Y Li, T Gopal, N Askanase, S Arumugam, S Mohan, PI Good, JS Stevens, F Lin, SK Sia, CS Lin, V D'Agati, K Kiryluk, NP Tatonetti, J Barasch
JCI Insight, 2022-03-22;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Hepatitis A Virus Cellular Receptor 1 (HAVcr-1) Initiates Prostate Cancer Progression in Human Cells via Hepatocyte Growth Factor (HGF)-Induced Changes in Junctional Integrity
Authors: EA Telford, AJ Sanders, S Owen, F Ruge, GM Harrison, WG Jiang, TA Martin
Biomolecules, 2022-02-21;12(2):.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Activation of Transcription Factor EB Alleviates Tubular Epithelial Cell Injury via Restoring Lysosomal Homeostasis in Diabetic Nephropathy
Authors: S Wang, K Jing, H Wu, X Li, C Yang, T Li, H Tang, T Zou, Y She, HF Liu
Oxidative Medicine and Cellular Longevity, 2022-01-12;2022(0):2812493.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Hydroxychloroquine administration exacerbates acute kidney injury complicated by lupus nephritis
Authors: N An, C Yang, HL Wu, Y Guo, XJ Huang, TS Huang, ZH Wu, J Xue, RH Chen, ZH Li, QJ Pan, HF Liu
Arthritis Research & Therapy, 2022-01-03;24(1):6.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Loss of renal olfactory receptor 1393 leads to improved glucose homeostasis in a type 1 diabetic mouse model
Authors: AR Schiazza, EG Considine, M Betcher, BD Shepard
Physiological Reports, 2021-12-01;9(23):e15007.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Aldehyde-driven transcriptional stress triggers an anorexic DNA damage response
Authors: L Mulderrig, JI Garaycoech, ZK Tuong, CL Millington, FA Dingler, JR Ferdinand, L Gaul, JA Tadross, MJ Arends, S O'Rahilly, GP Crossan, MR Clatworthy, KJ Patel
Nature, 2021-11-24;600(7887):158-163.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Farrerol Ameliorated Cisplatin-Induced Chronic Kidney Disease Through Mitophagy Induction via Nrf2/PINK1 Pathway
Authors: N Ma, Z Wei, J Hu, W Gu, X Ci
Frontiers in Pharmacology, 2021-11-11;12(0):768700.
Species: Mouse, Transgenic Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Deletion of Smad3 protects against C-reactive protein-induced renal fibrosis and inflammation in obstructive nephropathy
Authors: YK You, WF Wu, XR Huang, HD Li, YP Ren, JC Zeng, H Chen, HY Lan
International journal of biological sciences, 2021-09-21;17(14):3911-3922.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Kidney injury molecule-1 inhibits metastasis of renal cell carcinoma
Authors: JC Lee, DM Yotis, JY Lee, MA Sarabusky, B Shrum, A Champagne, OZ Ismail, E Tutunea-Fa, HS Leong, L Gunaratnam
Scientific Reports, 2021-06-04;11(1):11840.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Combination therapy of cisplatin with cilastatin enables an increased dose of cisplatin, enhancing its antitumor effect by suppression of nephrotoxicity
Authors: M Arita, S Watanabe, N Aoki, S Kuwahara, R Suzuki, S Goto, Y Abe, M Takahashi, M Sato, S Hokari, A Ohtsubo, S Shoji, K Nozaki, K Ichikawa, R Kondo, M Hayashi, Y Ohshima, H Kabasawa, M Hosojima, T Koya, A Saito, T Kikuchi
Scientific Reports, 2021-01-12;11(1):750.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The sodium-glucose cotransporter 2 inhibitor tofogliflozin prevents diabetic kidney disease progression in type 2 diabetic mice
Authors: Z Li, M Murakoshi, S Ichikawa, T Koshida, E Adachi, C Suzuki, S Ueda, T Gohda, Y Suzuki
FEBS Open Bio, 2020-11-10;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Vagus nerve stimulation even after injury ameliorates cisplatin-induced nephropathy via reducing macrophage infiltration
Authors: R Uni, T Inoue, Y Nakamura, D Fukaya, S Hasegawa, CH Wu, R Fujii, B Suratticha, W Peerapanya, A Ozeki, N Akimitsu, Y Wada, M Nangaku, R Inagi
Sci Rep, 2020-06-11;10(1):9472.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Signaling pathways predisposing to chronic kidney disease progression
Authors: M Zaidan, M Burtin, JD Zhang, T Blanc, P Barre, S Garbay, C Nguyen, F Vasseur, L Yammine, S Germano, L Badi, MC Gubler, M Gallazzini, G Friedlande, M Pontoglio, F Terzi
JCI Insight, 2020-05-07;5(9):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Phenformin Attenuates Renal Injury in Unilateral Ureteral Obstructed Mice without Affecting Immune Cell Infiltration
Authors: MØ Nørgård, M Christense, H A M Mutsae, R Nørregaard
Pharmaceutics, 2020-03-26;12(4):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
FOXM1 drives proximal tubule proliferation during repair from acute ischemic kidney injury
Authors: M Chang-Pane, FF Kadyrov, M Lalli, H Wu, S Ikeda, E Kefaloyian, MM Abdelmagee, A Herrlich, A Kobayashi, BD Humphreys
J. Clin. Invest., 2019-12-02;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Immunoglobulin light chains generate proinflammatory and profibrotic kidney injury
Authors: WZ Ying, X Li, S Rangarajan, W Feng, LM Curtis, PW Sanders
J. Clin. Invest., 2019-06-17;129(7):2792-2806.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Lack of Cathepsin D in the Renal Proximal Tubular Cells Resulted in Increased Sensitivity against Renal Ischemia/Reperfusion Injury
Authors: C Suzuki, I Tanida, M Ohmuraya, JA Oliva Trej, S Kakuta, T Sunabori, Y Uchiyama
Int J Mol Sci, 2019-04-05;20(7):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Control release of mitochondria-targeted antioxidant by injectable self-assembling peptide hydrogel ameliorated persistent mitochondrial dysfunction and inflammation after acute kidney injury
Authors: M Zhao, Y Zhou, S Liu, L Li, Y Chen, J Cheng, Y Lu, J Liu
Drug Deliv, 2018-11-01;25(1):546-554.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury
Authors: E Lazzeri, ML Angelotti, A Peired, C Conte, JA Marschner, L Maggi, B Mazzinghi, D Lombardi, ME Melica, S Nardi, E Ronconi, A Sisti, G Antonelli, F Becherucci, L De Chiara, RR Guevara, A Burger, B Schaefer, F Annunziato, HJ Anders, L Lasagni, P Romagnani
Nat Commun, 2018-04-09;9(1):1344.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy
Authors: O Maydan, PG McDade, Y Liu, XR Wu, DG Matsell, AA Eddy
Physiol Rep, 2018-03-01;6(6):e13654.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
PINK1-PRKN/PARK2 pathway of mitophagy is activated to protect against Renal ischemia-reperfusion injury
Authors: C Tang, H Han, M Yan, S Zhu, J Liu, Z Liu, L He, J Tan, Y Liu, H Liu, L Sun, S Duan, Y Peng, F Liu, XM Yin, Z Zhang, Z Dong
Autophagy, 2018-02-17;0(0):1-45.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Elevated urinary CRELD2 is associated with endoplasmic reticulum stress-mediated kidney disease
Authors: Y Kim, SJ Park, SR Manson, CA Molina, K Kidd, H Thiessen-P, RJ Perry, H Liapis, S Kmoch, CR Parikh, AJ Bleyer, YM Chen
JCI Insight, 2017-12-07;2(23):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Molecular characterization of the transition from acute to chronic kidney injury following ischemia/reperfusion
Authors: J Liu, S Kumar, E Dolzhenko, GF Alvarado, J Guo, C Lu, Y Chen, M Li, MC Dessing, RK Parvez, PE Cippà, AM Krautzberg, G Saribekyan, AD Smith, AP McMahon
JCI Insight, 2017-09-21;2(18):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Forkhead Box O3 (FoxO3) Regulates Kidney Tubular Autophagy Following Urinary Tract Obstruction
Authors: L Li, R Zviti, C Ha, ZV Wang, JA Hill, F Lin
J. Biol. Chem., 2017-07-13;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Rescue therapy with Tanshinone IIA hinders transition of acute kidney injury to chronic kidney disease via targeting GSK3?
Sci Rep, 2016-11-18;6(0):36698.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Rapid Renal Regulation of Peroxisome Proliferator-activated Receptor ? Coactivator-1? by Extracellular Signal-Regulated Kinase 1/2 in Physiological and Pathological Conditions
Authors: JB Collier, RM Whitaker, ST Eblen, RG Schnellman
J. Biol. Chem., 2016-11-14;291(52):26850-26859.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Wnt4 is a novel biomarker for the early detection of kidney tubular injury after ischemia/reperfusion injury
Sci Rep, 2016-09-07;6(0):32610.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Kidney-specific Sonoporation-mediated Gene Transfer.
Authors: Ishida R, Kami D, Kusaba T, Kirita Y, Kishida T, Mazda O, Adachi T, Gojo S
Mol Ther, 2015-09-30;24(1):125-34.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Characterizing functional domains for TIM-mediated enveloped virus entry.
Authors: Moller-Tank S, Albritton L, Rennert P, Maury W
J Virol, 2014-04-02;88(12):6702-13.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Long-term rescue of dystrophin expression and improvement in muscle pathology and function in dystrophic mdx mice by peptide-conjugated morpholino.
Am. J. Pathol., 2012-06-07;181(2):392-400.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Repair of injured proximal tubule does not involve specialized progenitors.
Authors: Humphreys BD, Czerniak S, DiRocco DP
Proc. Natl. Acad. Sci. U.S.A., 2011-05-16;108(22):9226-31.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells.
Authors: Nakae S, Iikura M, Suto H, Akiba H, Umetsu DT, DeKruyff RH, Saito H, Galli SJ
Blood, 2007-07-09;110(7):2565-8.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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