Mouse TIM-1/KIM-1/HAVCR Antibody
Mouse TIM-1/KIM-1/HAVCR Antibody Summary
Tyr22-Thr212
Accession # NP_001160104
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
View Larger
Detection of Mouse TIM‑1/KIM‑1/HAVCR by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1817) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TIM-1/KIM-1/HAVCR at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIM-1/KIM-1/HAVCR
TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVCR) is a 70-80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-4). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM genes (# 1-8) vs. only three genes in human (# 1, 3, and 4) (1, 2). Mouse TIM-1 and -2 are counterparts of human TIM-1 while mouse TIM-5 through 8 have no human counterparts (2). Mouse TIM-1 is synthesized as a 305 amino acid (aa) precursor that contains a 21 aa signal sequence, a 216 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (5, 6). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple T-S-P motifs. The mucin region undergoes extensive O-linked glycosylation. The mouse TIM-1 gene is highly polymorphic and, based on rat, may undergo alternate splicing (4, 6). For instance, HBA mice show a 15 aa deletion in the mucin region that occurs in BALB/c mice (6). This difference is associated with a decreased susceptibility to asthma. Other polymorphisms are also documented (6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing a 75-85 kDa soluble molecule (5). The same thing might be expected in mouse. The ECD of mouse TIM-1 is 50%, 39%, and 80% aa identical to human, canine and rat TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (8, 9). However, others are believed to exist, and based on the ligand for TIM-3, one possibility might be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10). In particular, it induces IL-4 production, and requires the cytoplasmic tyrosine phosphorylation motif (5).
- Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
- Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
- Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
- Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
- de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
- McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
- Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
- Feigelstock, D. et al. (1998) J. Virol. 72:6621.
- Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
- Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Product Datasheets
Citations for Mouse TIM-1/KIM-1/HAVCR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
22
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Long-term rescue of dystrophin expression and improvement in muscle pathology and function in dystrophic mdx mice by peptide-conjugated morpholino.
Am. J. Pathol., 2102;181(2):392-400.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Kidney injury molecule-1 inhibits metastasis of renal cell carcinoma
Authors: JC Lee, DM Yotis, JY Lee, MA Sarabusky, B Shrum, A Champagne, OZ Ismail, E Tutunea-Fa, HS Leong, L Gunaratnam
Scientific Reports, 2021;11(1):11840.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The sodium-glucose cotransporter 2 inhibitor tofogliflozin prevents diabetic kidney disease progression in type 2 diabetic mice
Authors: Z Li, M Murakoshi, S Ichikawa, T Koshida, E Adachi, C Suzuki, S Ueda, T Gohda, Y Suzuki
FEBS Open Bio, 2020;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Phenformin Attenuates Renal Injury in Unilateral Ureteral Obstructed Mice without Affecting Immune Cell Infiltration
Authors: MØ Nørgård, M Christense, H A M Mutsae, R Nørregaard
Pharmaceutics, 2020;12(4):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Signaling pathways predisposing to chronic kidney disease progression
Authors: M Zaidan, M Burtin, JD Zhang, T Blanc, P Barre, S Garbay, C Nguyen, F Vasseur, L Yammine, S Germano, L Badi, MC Gubler, M Gallazzini, G Friedlande, M Pontoglio, F Terzi
JCI Insight, 2020;5(9):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
FOXM1 drives proximal tubule proliferation during repair from acute ischemic kidney injury
Authors: M Chang-Pane, FF Kadyrov, M Lalli, H Wu, S Ikeda, E Kefaloyian, MM Abdelmagee, A Herrlich, A Kobayashi, BD Humphreys
J. Clin. Invest., 2019;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Immunoglobulin light chains generate proinflammatory and profibrotic kidney injury
Authors: WZ Ying, X Li, S Rangarajan, W Feng, LM Curtis, PW Sanders
J. Clin. Invest., 2019;129(7):2792-2806.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Lack of Cathepsin D in the Renal Proximal Tubular Cells Resulted in Increased Sensitivity against Renal Ischemia/Reperfusion Injury
Authors: C Suzuki, I Tanida, M Ohmuraya, JA Oliva Trej, S Kakuta, T Sunabori, Y Uchiyama
Int J Mol Sci, 2019;20(7):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Control release of mitochondria-targeted antioxidant by injectable self-assembling peptide hydrogel ameliorated persistent mitochondrial dysfunction and inflammation after acute kidney injury
Authors: M Zhao, Y Zhou, S Liu, L Li, Y Chen, J Cheng, Y Lu, J Liu
Drug Deliv, 2018;25(1):546-554.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy
Authors: O Maydan, PG McDade, Y Liu, XR Wu, DG Matsell, AA Eddy
Physiol Rep, 2018;6(6):e13654.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury
Authors: E Lazzeri, ML Angelotti, A Peired, C Conte, JA Marschner, L Maggi, B Mazzinghi, D Lombardi, ME Melica, S Nardi, E Ronconi, A Sisti, G Antonelli, F Becherucci, L De Chiara, RR Guevara, A Burger, B Schaefer, F Annunziato, HJ Anders, L Lasagni, P Romagnani
Nat Commun, 2018;9(1):1344.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Elevated urinary CRELD2 is associated with endoplasmic reticulum stress-mediated kidney disease
Authors: Y Kim, SJ Park, SR Manson, CA Molina, K Kidd, H Thiessen-P, RJ Perry, H Liapis, S Kmoch, CR Parikh, AJ Bleyer, YM Chen
JCI Insight, 2017;2(23):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
PINK1-PRKN/PARK2 pathway of mitophagy is activated to protect against Renal ischemia-reperfusion injury
Authors: C Tang, H Han, M Yan, S Zhu, J Liu, Z Liu, L He, J Tan, Y Liu, H Liu, L Sun, S Duan, Y Peng, F Liu, XM Yin, Z Zhang, Z Dong
Autophagy, 2017;0(0):1-45.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Molecular characterization of the transition from acute to chronic kidney injury following ischemia/reperfusion
Authors: J Liu, S Kumar, E Dolzhenko, GF Alvarado, J Guo, C Lu, Y Chen, M Li, MC Dessing, RK Parvez, PE Cippà, AM Krautzberg, G Saribekyan, AD Smith, AP McMahon
JCI Insight, 2017;2(18):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Forkhead Box O3 (FoxO3) Regulates Kidney Tubular Autophagy Following Urinary Tract Obstruction
Authors: L Li, R Zviti, C Ha, ZV Wang, JA Hill, F Lin
J. Biol. Chem., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Rescue therapy with Tanshinone IIA hinders transition of acute kidney injury to chronic kidney disease via targeting GSK3?
Sci Rep, 2016;6(0):36698.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Wnt4 is a novel biomarker for the early detection of kidney tubular injury after ischemia/reperfusion injury
Sci Rep, 2016;6(0):32610.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Rapid Renal Regulation of Peroxisome Proliferator-activated Receptor ? Coactivator-1? by Extracellular Signal-Regulated Kinase 1/2 in Physiological and Pathological Conditions
Authors: JB Collier, RM Whitaker, ST Eblen, RG Schnellman
J. Biol. Chem., 2016;291(52):26850-26859.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Kidney-specific Sonoporation-mediated Gene Transfer.
Authors: Ishida R, Kami D, Kusaba T, Kirita Y, Kishida T, Mazda O, Adachi T, Gojo S
Mol Ther, 2016;24(1):125-34.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Characterizing functional domains for TIM-mediated enveloped virus entry.
Authors: Moller-Tank S, Albritton L, Rennert P, Maury W
J Virol, 2014;88(12):6702-13.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Repair of injured proximal tubule does not involve specialized progenitors.
Authors: Humphreys BD, Czerniak S, DiRocco DP
Proc. Natl. Acad. Sci. U.S.A., 2011;108(22):9226-31.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells.
Authors: Nakae S, Iikura M, Suto H, Akiba H, Umetsu DT, DeKruyff RH, Saito H, Galli SJ
Blood, 2007;110(7):2565-8.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Mouse TIM-1/KIM-1/HAVCR Antibody
Average Rating: 5 (Based on 1 Review)
Have you used Mouse TIM-1/KIM-1/HAVCR Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
