Mouse TLR6 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse TLR6. Stains mouse TLR6 transfectants and not irrelevant transfectants.
  • Source
    Monoclonal Rat IgG2A Clone # 418601
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    HEK293 human embryonic kidney cell line transfected with mouse TLR6
    Accession # BAA78632
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TLR6 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse TLR6 APC‑conjugated Monoclonal Antibody (Catalog # FAB1533A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TLR6

The Toll-like family of molecules are a group of integral membrane proteins that serve as pattern recognition receptors for microbial pathogens. There are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (1‑4). All Toll-like receptors (TLRs) are type I transmembrane (TM) proteins that exist either in the plasma membrane or in the membranes of endosomal structures (where they bind intracellular microbial nucleic acids). All TLRs also contain a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL-1 receptor (TIR) domain. The mouse TLR6 cDNA encodes a 795 amino acid (aa) precursor that includes a 27 aa signal sequence, a 557 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 190 aa cytoplasmic domain. The ECD contains 14 Leu-rich repeats, and the cytoplasmic region contains one TIR domain (5). Within the ECD, mouse TLR6 shares 59% aa sequence identity with mouse TLR1 and 20‑27% aa sequence identitity with mouse TLR2, -3, -4, -5, -7, -8, -9, -11, -12, and -13. It shares 71%, 72%, and 86% aa sequence identity with bovine, human, and rat TLR6, respectively. TLR6 is expressed on the cell surface of macrophages, monocytes, neutrophils, and dermal endothelial cells in ligand-independent association with TLR2 (6‑9). TLR2 also associates with TLR1, a functional complex with specificity for distinct but related microbial ligands (6‑8). TLR6 and TLR2 cooperate in the recognition of acylated bacterial and mycoplasma lipopeptides, peptidoglycan, and glycosylphosphatidylinositols (7‑14). The cytoplasmic TIR domain is necessary and sufficient to initiate signal transduction which leads to activation of NF kappa B (7, 15).

  • References:
    1. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
    2. Roeder, A. et al. (2004) Med. Mycol. 42:485.
    3. Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
    4. Wetzler, L.M. (2003) Vaccine 21:S55.
    5. Takeuchi, O. et al. (1999) Gene 231:59.
    6. Hajjar, A.M. et al. (2001) J. Immunol. 166:15.
    7. Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:13766.
    8. Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
    9. Nakao, Y. et al. (2005) J. Immunol. 174:1566.
    10. Bulut, Y. et al. (2001) J. Immunol. 167:987.
    11. Takeuchi, O. et al. (2001) Int. Immunol. 13:933.
    12. Morr, M. et al. (2002) Eur. J. Immunol. 32:3337.
    13. Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
    14. Omueti, K.O. et al. (2005) J. Biol. Chem. 280:36616.
    15. Nishiya, T. and A.L. DeFranco (2004) J. Biol. Chem. 279:19008.
  • Long Name:
    Toll-like Receptor 6
  • Entrez Gene IDs:
    10333 (Human); 21899 (Mouse); 305353 (Rat)
  • Alternate Names:
    CD286 antigen; CD286; toll-like receptor 6
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Allophycocyanin Isotype Control

Ctrl IC006A 4  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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