Mouse TWEAK/TNFSF12 Antibody

Catalog # Availability Size / Price Qty
MAB1237
MAB1237-SP
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Mouse TWEAK/TNFSF12 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TWEAK/TNFSF12 in ELISAs. In sandwich ELISAs, approximately 5% cross-reactivity with recombinant human TWEAK is observed and no cross-reactivity with recombinant mouse (rm) BAFF, rmFas Ligand, rmOX40, or rmTNF-alpha is observed.
Source
Monoclonal Rat IgG2A Clone # 189803
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse TWEAK/TNFSF12
Arg105-His249
Accession # O54907.2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample

Mouse TWEAK/TNFSF12 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse TWEAK/TNFSF12 Biotinylated Antibody (Catalog # BAF1237)

Standard: Recombinant Mouse TWEAK/TNFSF12 Protein (Catalog # 1237-TW)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TWEAK/TNFSF12

TNF-related weak inducer of apoptosis (TWEAK) is a type II transmembrane protein belonging to the TNF superfamily and has been designated TNFSF12. Mouse TWEAK is a 249 amino acid (aa) protein with an N-terminal 21 aa cytoplasmic domain, a 21 aa transmembrane region and a 204 aa C-terminal extracellular domain (1). Human and mouse TWEAK share 88% amino acid sequence identity within the extracellular domain. A soluble form of TWEAK is generated from the membrane-associated molecules by proteolytic cleavage suggesting that TWEAK may have long-range effects. TWEAK is expressed widely in many tissues and cells (1). Although TWEAK has been proposed as a ligand that signals through the death domain receptor 3 (DR3) (2), a TNF receptor superfamily member currently designated TNFRSF25, subsequent studies did not demonstrate binding of TWEAK to cell lines that express DR3 (3). In cells that lack DR3, TWEAK has been shown to bind TWEAK receptor (TWEAK R), a novel TNF receptor superfamily member designated TNFRSF12A (4‑7). TWEAK R, also known as fibroblast growth factor-inducible 14 (Fn14), is a growth factor-inducible immediate-early response gene that is expressed in fibroblasts, hepatocellular carcinomas and endothelial cells. TWEAK-TWEAK R interaction has been shown to promote NF-kappa B activation and mediate multiple cell death pathways. On endothelial cells, TWEAK R plays a role in endothelial cell growth and migration. This effect of TWEAK is not due to upregulation of VEGF (8).

References
  1. Chicheportiche, Y. et al. (1997) J. Biol Chem. 272:32401.
  2. Marsters, S. et al. (1998) Current Biol. 8:525.
  3. Kaptein, A. et al. (2000) FEBS Letters, 485:135.
  4. Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.
  5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
  6. Feng, S.L. et al. (2000) Am. J. Pathol. 156:1253.
  7. Wiley, S.R. et al. (2001) Immunity 15:837.
  8. Lynch, C. et al. (1998) J. Biol. Chem. 274:8455.
Long Name
TNF-like and Weak Inducer of Apoptosis
Entrez Gene IDs
8742 (Human); 21944 (Mouse); 360548 (Rat)
Alternate Names
APO3 ligand; APO3LMGC20669; DR3LGTWEAKAPO3/DR3 ligand; MGC129581; TNF-related weak inducer of apoptosis; TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand superfamily member 12; TWEAK

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