Mouse uPAR Antibody

Catalog # Availability Size / Price Qty
AF534
AF534-SP
uPAR in Mouse Kidney.
25 Images
Product Details
Citations (16)
FAQs
Supplemental Products
Reviews (1)

Mouse uPAR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse uPAR in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse uPAR isoform 1
Leu24-Thr297
Accession # Q545X5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse uPAR Fc Chimera (Catalog # 531-PA)
Flow Cytometry
0.25 µg/106 cells
RAW 264.7 mouse monocyte/macrophage cell line
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.8-4 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human u-Plasminogen Activator/Urokinase (Catalog # 1310-SE) to immobilized Recombinant Mouse uPAR Fc Chimera (Catalog # 531-PA) coated at 5 µg/mL (100 µL/well). At 25 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry uPAR antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

uPAR in Mouse Kidney. uPAR was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF534) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Flow Cytometry View Larger

Detection of uPAR in Raw264.7 cells by Flow Cytometry. Raw264.7 cells were stained with Goat Anti-Mouse uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF534, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Mouse uPAR by Flow Cytometry View Larger

Detection of Mouse uPAR by Flow Cytometry Expression of murine Plaur in AT84 cells.In vitro characterization of AT84 cells stably transfected with either empty vector (EV) or a vector containing cDNA encoding murine uPAR (Plaur). A: Western blot analysis of whole cell lysates using a polyclonal anti-murine uPAR antibody (AF534). A total of 7.5 ng of recombinant murine uPAR (rmuPAR) was loaded as a positive control. Re-probing for beta -actin was used as a loading control. B: Western blot analysis of cellular membrane fractions using a polyclonal anti-murine uPAR antibody (AF534). Total protein was measured per sample and 53.5 µg of protein was loaded per lane. A and B: Images were cropped, as no additional bands were detectable. C: FACS analysis of non-permeabilized cells using a polyclonal anti-murine uPAR antibody (AF534). Alexa Fluor 488 anti-goat secondary antibody (A11055) was used as the secondary antibody. The quantified mean Alexa 488 fluorescence signal per cell line is presented in the panel to the right. D and E: Relative Plaur mRNA (uPAR) (D) or Plau mRNA (uPA) (E) expression levels as analysed using RT-qPCR. All expression levels were normalized to the expression of the reference genes Trfc and beta -actin. Error bars represent the standard error of mean (+SEM) and N = 3. One-way ANOVA; *p<0.05. F: Plasminogen-gelatin (upper panel) and gelatin (lower panel) zymography analysis of conditioned medium of cells cultured for 24 hours in SFM. HMW-uPA and mPLM (mouse plasmin) were loaded as positive controls. The images were cropped to size. G: Relative Plasminogen mRNA (Plg) expression levels as analysed using RT-qPCR. Error bars represent standard error of mean (+SEM) and N = 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse uPAR by Western Blot View Larger

Detection of Mouse uPAR by Western Blot Leiomyoma conditioned medium induced uPAR expression.Analysis of uPAR expression induced by the LCM or purified ECM proteins in cultured uPAR knock-down cells. All Western blots were performed on whole cell lysates, and uPAR was detected using the polyclonal anti-murine uPAR antibody (AF534). A: Cells were either cultured in LCM (LM) or serum free medium (SF) for 24 hours. Cells were harvested with sample buffer and re-probing for beta -actin was used as a loading control. B: uPAR1-NT cells were seeded on different ECM protein substrates, incubated for 24 hours and harvested using RIPA buffer. 7.5 µg of total protein was loaded per lane. Equal loading was verified by re-probing for beta -actin. The poloxamer pluronic was used as a no-adhesion control. Col I = Collagen I, Vn = Vitronectin, Fn = Fibronectin, Lm = Laminin, ECL = Entactin, Collagen, Laminin, and FBS = Foetal Bovine Serum. C: Cells cultured in LCM (LM) or serum free medium (SF) for 24 hours were harvested using sample buffer and deglycosylated by PNGase F treatment (+) as indicated. Re-probing for beta -actin was used as a loading control. The three bands detected by the anti-uPAR antibody are labelled 1, 2, and 3, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse uPAR by Immunohistochemistry View Larger

Detection of Mouse uPAR by Immunohistochemistry Tumour microenvironment induced uPAR protein expression in tongue tumours.Tumour growth pattern and uPAR protein levels in tongue tumours generated from the EV1, EV2, uPAR1 and uPAR2 cells. A–B: Representative images depicting the tumour growth pattern at the tumour-stroma interface in hematoxylin/eosin stained EV1 (A) and uPAR1 (B) tumours. Images were recorded at 10x magnification. C–D: Representative images depicting the IHC uPAR staining of the EV1 (C) or uPAR1 tumours (D). Images were recorded at 4x magnification. E–H: The images show high power magnification (20x magnifications) of the EV1 (E), uPAR1 (F), EV2 (G) and uPAR2 (H) tumours IHC stained for uPAR protein. Positive uPAR staining is seen as brown colour, and counterstaining was done with haematoxylin. I: The average staining index (SI) of the uPAR staining in the tumours. Maximum obtainable score is 9. The error bars shows the +SEM. N = number of tumours; EV1, N = 8/10; EV2, N = 5/10; uPAR1, N = 4/10; uPAR2 N = 9/10. One-way ANOVA; **p<0.01, *p<0.05. T = Tumours, S = Stroma. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse uPAR by Immunohistochemistry View Larger

Detection of Mouse uPAR by Immunohistochemistry Leiomyoma stroma is a strong inducer of uPAR expression.Representative images of low- (EV1-sh3) and high- (uPAR1-NT) uPAR-expressing cells invading the ex vivo leiomyoma tissue. Cells were incubated for 7 and 14 days, as indicated. The tissue was IHC stained for uPAR. Positive uPAR staining is seen as brown colour, counterstained with haematoxylin. Images were recorded at 10x magnification. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse uPAR by Immunohistochemistry View Larger

Detection of Mouse uPAR by Immunohistochemistry Tumour microenvironment induced uPAR protein expression in tongue tumours.Tumour growth pattern and uPAR protein levels in tongue tumours generated from the EV1, EV2, uPAR1 and uPAR2 cells. A–B: Representative images depicting the tumour growth pattern at the tumour-stroma interface in hematoxylin/eosin stained EV1 (A) and uPAR1 (B) tumours. Images were recorded at 10x magnification. C–D: Representative images depicting the IHC uPAR staining of the EV1 (C) or uPAR1 tumours (D). Images were recorded at 4x magnification. E–H: The images show high power magnification (20x magnifications) of the EV1 (E), uPAR1 (F), EV2 (G) and uPAR2 (H) tumours IHC stained for uPAR protein. Positive uPAR staining is seen as brown colour, and counterstaining was done with haematoxylin. I: The average staining index (SI) of the uPAR staining in the tumours. Maximum obtainable score is 9. The error bars shows the +SEM. N = number of tumours; EV1, N = 8/10; EV2, N = 5/10; uPAR1, N = 4/10; uPAR2 N = 9/10. One-way ANOVA; **p<0.01, *p<0.05. T = Tumours, S = Stroma. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse uPAR by Immunohistochemistry View Larger

Detection of Mouse uPAR by Immunohistochemistry Tumour microenvironment induced uPAR protein expression in tongue tumours.Tumour growth pattern and uPAR protein levels in tongue tumours generated from the EV1, EV2, uPAR1 and uPAR2 cells. A–B: Representative images depicting the tumour growth pattern at the tumour-stroma interface in hematoxylin/eosin stained EV1 (A) and uPAR1 (B) tumours. Images were recorded at 10x magnification. C–D: Representative images depicting the IHC uPAR staining of the EV1 (C) or uPAR1 tumours (D). Images were recorded at 4x magnification. E–H: The images show high power magnification (20x magnifications) of the EV1 (E), uPAR1 (F), EV2 (G) and uPAR2 (H) tumours IHC stained for uPAR protein. Positive uPAR staining is seen as brown colour, and counterstaining was done with haematoxylin. I: The average staining index (SI) of the uPAR staining in the tumours. Maximum obtainable score is 9. The error bars shows the +SEM. N = number of tumours; EV1, N = 8/10; EV2, N = 5/10; uPAR1, N = 4/10; uPAR2 N = 9/10. One-way ANOVA; **p<0.01, *p<0.05. T = Tumours, S = Stroma. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse uPAR by Immunohistochemistry View Larger

Detection of Mouse uPAR by Immunohistochemistry Tumour microenvironment induced uPAR protein expression in tongue tumours.Tumour growth pattern and uPAR protein levels in tongue tumours generated from the EV1, EV2, uPAR1 and uPAR2 cells. A–B: Representative images depicting the tumour growth pattern at the tumour-stroma interface in hematoxylin/eosin stained EV1 (A) and uPAR1 (B) tumours. Images were recorded at 10x magnification. C–D: Representative images depicting the IHC uPAR staining of the EV1 (C) or uPAR1 tumours (D). Images were recorded at 4x magnification. E–H: The images show high power magnification (20x magnifications) of the EV1 (E), uPAR1 (F), EV2 (G) and uPAR2 (H) tumours IHC stained for uPAR protein. Positive uPAR staining is seen as brown colour, and counterstaining was done with haematoxylin. I: The average staining index (SI) of the uPAR staining in the tumours. Maximum obtainable score is 9. The error bars shows the +SEM. N = number of tumours; EV1, N = 8/10; EV2, N = 5/10; uPAR1, N = 4/10; uPAR2 N = 9/10. One-way ANOVA; **p<0.01, *p<0.05. T = Tumours, S = Stroma. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse uPAR by Western Blot View Larger

Detection of Mouse uPAR by Western Blot TGF-beta 1 reduces uPAR cleavage through induced PAI-1 expression. a, f-h. Western blot analysis of whole cell lysates of cultured and stimulated AT84-uPAR cells. Where indicated, cell lysates were either treated with PNGase F (+) or samples received the same treatment except that PNGase F was omitted (−). Glycosylated uPAR is indicated as uPARglc. d-e. Western blot analysis of conditioned medium from equally seeded amounts of AT84-EV and AT84-uPAR cells. a. Cells cultured in FBSM with or without 2 ng/ml active human TGF-beta 1 for 24 h. b-c. Total RNA from treated (TGF-beta 1) or untreated (Ctrl) cells was isolated and the relative expression of uPAR mRNA (B) or uPA mRNA (C) was analysed using RT-qPCR. The error bars show the +SD. N = 3. d. Cells cultured in SFM or FBSM and stimulated with 2 ng/ml TGF-beta 1 for 24 h as indicated and PAI-1 protein levels were analysed. Controls received either no additives (−) or the TGF-beta 1 buffer (0). Positive control: recombinant mouse PAI-1 (rmPAI-1). e. AT84-uPAR cells cultured in either SFM or in FBSM. Cells were either unstimulated (−) or stimulated (+) with 2 ng/ml TGF-beta 1 and/or 10 μM of the TGF-beta 1-inhibitor SB431542 as indicated. f. AT84-uPAR cells were cultured for 24 h in FBSM and stimulated with 2 ng/ml TGF-beta 1 as indicated. g. Deglycosylated whole cell lysates from AT84-uPAR cells cultured in SFM stimulated with 2 ng/ml TGF-beta 1 +/− the inhibitor SB431542 as indicated were analysed for uPAR protein levels. h. Cells were cultured for 24- or 48 h in SFM, FBSM or TMEM. Cells were treated with 2 ng/ml TGF-beta 1 in 10% FBS as indicated. The LRP1 protein is indicated. Arrowhead shows an unknown band. Image collected and cropped by CiteAb from the following open publication (https://bmccancer.biomedcentral.com/articles/10.1186/s12885-017-3349-7), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse uPAR by Western Blot View Larger

Detection of Mouse uPAR by Western Blot TGF-beta 1 reduces uPAR cleavage through induced PAI-1 expression. a, f-h. Western blot analysis of whole cell lysates of cultured and stimulated AT84-uPAR cells. Where indicated, cell lysates were either treated with PNGase F (+) or samples received the same treatment except that PNGase F was omitted (−). Glycosylated uPAR is indicated as uPARglc. d-e. Western blot analysis of conditioned medium from equally seeded amounts of AT84-EV and AT84-uPAR cells. a. Cells cultured in FBSM with or without 2 ng/ml active human TGF-beta 1 for 24 h. b-c. Total RNA from treated (TGF-beta 1) or untreated (Ctrl) cells was isolated and the relative expression of uPAR mRNA (B) or uPA mRNA (C) was analysed using RT-qPCR. The error bars show the +SD. N = 3. d. Cells cultured in SFM or FBSM and stimulated with 2 ng/ml TGF-beta 1 for 24 h as indicated and PAI-1 protein levels were analysed. Controls received either no additives (−) or the TGF-beta 1 buffer (0). Positive control: recombinant mouse PAI-1 (rmPAI-1). e. AT84-uPAR cells cultured in either SFM or in FBSM. Cells were either unstimulated (−) or stimulated (+) with 2 ng/ml TGF-beta 1 and/or 10 μM of the TGF-beta 1-inhibitor SB431542 as indicated. f. AT84-uPAR cells were cultured for 24 h in FBSM and stimulated with 2 ng/ml TGF-beta 1 as indicated. g. Deglycosylated whole cell lysates from AT84-uPAR cells cultured in SFM stimulated with 2 ng/ml TGF-beta 1 +/− the inhibitor SB431542 as indicated were analysed for uPAR protein levels. h. Cells were cultured for 24- or 48 h in SFM, FBSM or TMEM. Cells were treated with 2 ng/ml TGF-beta 1 in 10% FBS as indicated. The LRP1 protein is indicated. Arrowhead shows an unknown band. Image collected and cropped by CiteAb from the following open publication (https://bmccancer.biomedcentral.com/articles/10.1186/s12885-017-3349-7), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse uPAR by Immunohistochemistry View Larger

Detection of Mouse uPAR by Immunohistochemistry Tumour microenvironment induced uPAR protein expression in tongue tumours.Tumour growth pattern and uPAR protein levels in tongue tumours generated from the EV1, EV2, uPAR1 and uPAR2 cells. A–B: Representative images depicting the tumour growth pattern at the tumour-stroma interface in hematoxylin/eosin stained EV1 (A) and uPAR1 (B) tumours. Images were recorded at 10x magnification. C–D: Representative images depicting the IHC uPAR staining of the EV1 (C) or uPAR1 tumours (D). Images were recorded at 4x magnification. E–H: The images show high power magnification (20x magnifications) of the EV1 (E), uPAR1 (F), EV2 (G) and uPAR2 (H) tumours IHC stained for uPAR protein. Positive uPAR staining is seen as brown colour, and counterstaining was done with haematoxylin. I: The average staining index (SI) of the uPAR staining in the tumours. Maximum obtainable score is 9. The error bars shows the +SEM. N = number of tumours; EV1, N = 8/10; EV2, N = 5/10; uPAR1, N = 4/10; uPAR2 N = 9/10. One-way ANOVA; **p<0.01, *p<0.05. T = Tumours, S = Stroma. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse uPAR by Western Blot View Larger

Detection of Mouse uPAR by Western Blot Leiomyoma conditioned medium induced uPAR expression.Analysis of uPAR expression induced by the LCM or purified ECM proteins in cultured uPAR knock-down cells. All Western blots were performed on whole cell lysates, and uPAR was detected using the polyclonal anti-murine uPAR antibody (AF534). A: Cells were either cultured in LCM (LM) or serum free medium (SF) for 24 hours. Cells were harvested with sample buffer and re-probing for beta -actin was used as a loading control. B: uPAR1-NT cells were seeded on different ECM protein substrates, incubated for 24 hours and harvested using RIPA buffer. 7.5 µg of total protein was loaded per lane. Equal loading was verified by re-probing for beta -actin. The poloxamer pluronic was used as a no-adhesion control. Col I = Collagen I, Vn = Vitronectin, Fn = Fibronectin, Lm = Laminin, ECL = Entactin, Collagen, Laminin, and FBS = Foetal Bovine Serum. C: Cells cultured in LCM (LM) or serum free medium (SF) for 24 hours were harvested using sample buffer and deglycosylated by PNGase F treatment (+) as indicated. Re-probing for beta -actin was used as a loading control. The three bands detected by the anti-uPAR antibody are labelled 1, 2, and 3, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse uPAR by Western Blot View Larger

Detection of Mouse uPAR by Western Blot Expression of murine Plaur in AT84 cells.In vitro characterization of AT84 cells stably transfected with either empty vector (EV) or a vector containing cDNA encoding murine uPAR (Plaur). A: Western blot analysis of whole cell lysates using a polyclonal anti-murine uPAR antibody (AF534). A total of 7.5 ng of recombinant murine uPAR (rmuPAR) was loaded as a positive control. Re-probing for beta -actin was used as a loading control. B: Western blot analysis of cellular membrane fractions using a polyclonal anti-murine uPAR antibody (AF534). Total protein was measured per sample and 53.5 µg of protein was loaded per lane. A and B: Images were cropped, as no additional bands were detectable. C: FACS analysis of non-permeabilized cells using a polyclonal anti-murine uPAR antibody (AF534). Alexa Fluor 488 anti-goat secondary antibody (A11055) was used as the secondary antibody. The quantified mean Alexa 488 fluorescence signal per cell line is presented in the panel to the right. D and E: Relative Plaur mRNA (uPAR) (D) or Plau mRNA (uPA) (E) expression levels as analysed using RT-qPCR. All expression levels were normalized to the expression of the reference genes Trfc and beta -actin. Error bars represent the standard error of mean (+SEM) and N = 3. One-way ANOVA; *p<0.05. F: Plasminogen-gelatin (upper panel) and gelatin (lower panel) zymography analysis of conditioned medium of cells cultured for 24 hours in SFM. HMW-uPA and mPLM (mouse plasmin) were loaded as positive controls. The images were cropped to size. G: Relative Plasminogen mRNA (Plg) expression levels as analysed using RT-qPCR. Error bars represent standard error of mean (+SEM) and N = 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse uPAR by Immunohistochemistry View Larger

Detection of Mouse uPAR by Immunohistochemistry Tumour microenvironment induced uPAR protein expression in tongue tumours.Tumour growth pattern and uPAR protein levels in tongue tumours generated from the EV1, EV2, uPAR1 and uPAR2 cells. A–B: Representative images depicting the tumour growth pattern at the tumour-stroma interface in hematoxylin/eosin stained EV1 (A) and uPAR1 (B) tumours. Images were recorded at 10x magnification. C–D: Representative images depicting the IHC uPAR staining of the EV1 (C) or uPAR1 tumours (D). Images were recorded at 4x magnification. E–H: The images show high power magnification (20x magnifications) of the EV1 (E), uPAR1 (F), EV2 (G) and uPAR2 (H) tumours IHC stained for uPAR protein. Positive uPAR staining is seen as brown colour, and counterstaining was done with haematoxylin. I: The average staining index (SI) of the uPAR staining in the tumours. Maximum obtainable score is 9. The error bars shows the +SEM. N = number of tumours; EV1, N = 8/10; EV2, N = 5/10; uPAR1, N = 4/10; uPAR2 N = 9/10. One-way ANOVA; **p<0.01, *p<0.05. T = Tumours, S = Stroma. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse uPAR by Western Blot View Larger

Detection of Mouse uPAR by Western Blot Soluble uPAR shed from mouse OSCC cells induce migration. a-b. Western blot analysis of uPAR protein levels in concentrated conditioned medium from AT84-EV and AT84-uPAR cells. a. Conditioned medium from cells cultured in SFM or SFM containing 10 nM plasminogen (plg) as indicated. The conditioned medium was concentrated from 2 ml to 110 μl for both samples, and deglycosylated using PNGase F. b. Conditioned medium from cells cultured in SFM or in FBSM was concentrated from 2 ml to 350 μl for each sample. A whole cell lysate sample of AT84-uPAR cells was used as a positive control. The two indicated FBSM samples are replicates. Glycosylated suPAR is indicated as suPARglc. c. Non-concentrated conditioned medium from AT84-EV (EV medium) and AT84-uPAR cells (uPAR-medium) was used as an attractant for the AT84-EV cells in a real-time cell migration analysis using the xCELLigence system. N = 3, where each experiment had two technical replicates, and the error bars represent the standard error of mean (±SEM) based on three separate experiments. Image collected and cropped by CiteAb from the following open publication (https://bmccancer.biomedcentral.com/articles/10.1186/s12885-017-3349-7), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse uPAR by Immunohistochemistry View Larger

Detection of Mouse uPAR by Immunohistochemistry In vivo tongue tumours of EV1 and uPAR1 knock-down cells.IHC uPAR staining and growth pattern of tongue tumours generated from the EV1 and uPAR1 cells containing either shRNA targeting uPAR (EV1-sh and uPAR1-sh), or non-targeting shRNA (uPAR1-NT). A–C: IHC uPAR staining of EV1-sh (A), uPAR1-NT (B) and uPAR1-sh (C) tumours, respectively. Images were recorded at 20x magnifications. D: Representative image depicting the tumour growth pattern at the tumour-stroma interface in hematoxylin/eosin stained EV1-sh. E: The average SI of the uPAR staining in the tumours, with the maximum obtainable score of 9. The error bars shows the +SEM. N = number of tumours; EV1, N = 8/10; EV1-sh, N = 11/16; uPAR1, N = 4/10; uPAR1-NT, N = 3/8; uPAR1-sh, N = 4/16. One-way ANOVA; **p<0.01, *p<0.05. T = Tumour, S = Stroma. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse uPAR by Immunohistochemistry View Larger

Detection of Mouse uPAR by Immunohistochemistry In vivo tongue tumours of EV1 and uPAR1 knock-down cells.IHC uPAR staining and growth pattern of tongue tumours generated from the EV1 and uPAR1 cells containing either shRNA targeting uPAR (EV1-sh and uPAR1-sh), or non-targeting shRNA (uPAR1-NT). A–C: IHC uPAR staining of EV1-sh (A), uPAR1-NT (B) and uPAR1-sh (C) tumours, respectively. Images were recorded at 20x magnifications. D: Representative image depicting the tumour growth pattern at the tumour-stroma interface in hematoxylin/eosin stained EV1-sh. E: The average SI of the uPAR staining in the tumours, with the maximum obtainable score of 9. The error bars shows the +SEM. N = number of tumours; EV1, N = 8/10; EV1-sh, N = 11/16; uPAR1, N = 4/10; uPAR1-NT, N = 3/8; uPAR1-sh, N = 4/16. One-way ANOVA; **p<0.01, *p<0.05. T = Tumour, S = Stroma. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse uPAR by Western Blot View Larger

Detection of Mouse uPAR by Western Blot Knock-down of uPAR expression in AT84 cells.shRNA knock-down of Plaur in AT84 cells. A: Flow chart showing the generation of the single cell clones. B: Western blot analysis of whole cell lysates from the single cell clones stably transfected with either shRNA-constructs (shRNA 3 = sh3, shRNA 4 = sh4 or shRNA 5 = sh5) targeting Plaur or constructs containing non-target shRNA (NT) or the empty vector (EV). uPAR was detected using a polyclonal anti-murine uPAR antibody (AF534). Re-probing for beta -actin was used as a loading control. Images were cropped, as no additional bands were detected in the blot. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse uPAR by Western Blot View Larger

Detection of Mouse uPAR by Western Blot TGF-beta 1 reduces uPAR cleavage through induced PAI-1 expression. a, f-h. Western blot analysis of whole cell lysates of cultured and stimulated AT84-uPAR cells. Where indicated, cell lysates were either treated with PNGase F (+) or samples received the same treatment except that PNGase F was omitted (−). Glycosylated uPAR is indicated as uPARglc. d-e. Western blot analysis of conditioned medium from equally seeded amounts of AT84-EV and AT84-uPAR cells. a. Cells cultured in FBSM with or without 2 ng/ml active human TGF-beta 1 for 24 h. b-c. Total RNA from treated (TGF-beta 1) or untreated (Ctrl) cells was isolated and the relative expression of uPAR mRNA (B) or uPA mRNA (C) was analysed using RT-qPCR. The error bars show the +SD. N = 3. d. Cells cultured in SFM or FBSM and stimulated with 2 ng/ml TGF-beta 1 for 24 h as indicated and PAI-1 protein levels were analysed. Controls received either no additives (−) or the TGF-beta 1 buffer (0). Positive control: recombinant mouse PAI-1 (rmPAI-1). e. AT84-uPAR cells cultured in either SFM or in FBSM. Cells were either unstimulated (−) or stimulated (+) with 2 ng/ml TGF-beta 1 and/or 10 μM of the TGF-beta 1-inhibitor SB431542 as indicated. f. AT84-uPAR cells were cultured for 24 h in FBSM and stimulated with 2 ng/ml TGF-beta 1 as indicated. g. Deglycosylated whole cell lysates from AT84-uPAR cells cultured in SFM stimulated with 2 ng/ml TGF-beta 1 +/− the inhibitor SB431542 as indicated were analysed for uPAR protein levels. h. Cells were cultured for 24- or 48 h in SFM, FBSM or TMEM. Cells were treated with 2 ng/ml TGF-beta 1 in 10% FBS as indicated. The LRP1 protein is indicated. Arrowhead shows an unknown band. Image collected and cropped by CiteAb from the following open publication (https://bmccancer.biomedcentral.com/articles/10.1186/s12885-017-3349-7), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse uPAR by Western Blot View Larger

Detection of Mouse uPAR by Western Blot Soluble uPAR shed from mouse OSCC cells induce migration. a-b. Western blot analysis of uPAR protein levels in concentrated conditioned medium from AT84-EV and AT84-uPAR cells. a. Conditioned medium from cells cultured in SFM or SFM containing 10 nM plasminogen (plg) as indicated. The conditioned medium was concentrated from 2 ml to 110 μl for both samples, and deglycosylated using PNGase F. b. Conditioned medium from cells cultured in SFM or in FBSM was concentrated from 2 ml to 350 μl for each sample. A whole cell lysate sample of AT84-uPAR cells was used as a positive control. The two indicated FBSM samples are replicates. Glycosylated suPAR is indicated as suPARglc. c. Non-concentrated conditioned medium from AT84-EV (EV medium) and AT84-uPAR cells (uPAR-medium) was used as an attractant for the AT84-EV cells in a real-time cell migration analysis using the xCELLigence system. N = 3, where each experiment had two technical replicates, and the error bars represent the standard error of mean (±SEM) based on three separate experiments. Image collected and cropped by CiteAb from the following open publication (https://bmccancer.biomedcentral.com/articles/10.1186/s12885-017-3349-7), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse uPAR by Western Blot View Larger

Detection of Mouse uPAR by Western Blot Expression of murine Plaur in AT84 cells.In vitro characterization of AT84 cells stably transfected with either empty vector (EV) or a vector containing cDNA encoding murine uPAR (Plaur). A: Western blot analysis of whole cell lysates using a polyclonal anti-murine uPAR antibody (AF534). A total of 7.5 ng of recombinant murine uPAR (rmuPAR) was loaded as a positive control. Re-probing for beta -actin was used as a loading control. B: Western blot analysis of cellular membrane fractions using a polyclonal anti-murine uPAR antibody (AF534). Total protein was measured per sample and 53.5 µg of protein was loaded per lane. A and B: Images were cropped, as no additional bands were detectable. C: FACS analysis of non-permeabilized cells using a polyclonal anti-murine uPAR antibody (AF534). Alexa Fluor 488 anti-goat secondary antibody (A11055) was used as the secondary antibody. The quantified mean Alexa 488 fluorescence signal per cell line is presented in the panel to the right. D and E: Relative Plaur mRNA (uPAR) (D) or Plau mRNA (uPA) (E) expression levels as analysed using RT-qPCR. All expression levels were normalized to the expression of the reference genes Trfc and beta -actin. Error bars represent the standard error of mean (+SEM) and N = 3. One-way ANOVA; *p<0.05. F: Plasminogen-gelatin (upper panel) and gelatin (lower panel) zymography analysis of conditioned medium of cells cultured for 24 hours in SFM. HMW-uPA and mPLM (mouse plasmin) were loaded as positive controls. The images were cropped to size. G: Relative Plasminogen mRNA (Plg) expression levels as analysed using RT-qPCR. Error bars represent standard error of mean (+SEM) and N = 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse uPAR by Western Blot View Larger

Detection of Mouse uPAR by Western Blot Leiomyoma conditioned medium induced uPAR expression.Analysis of uPAR expression induced by the LCM or purified ECM proteins in cultured uPAR knock-down cells. All Western blots were performed on whole cell lysates, and uPAR was detected using the polyclonal anti-murine uPAR antibody (AF534). A: Cells were either cultured in LCM (LM) or serum free medium (SF) for 24 hours. Cells were harvested with sample buffer and re-probing for beta -actin was used as a loading control. B: uPAR1-NT cells were seeded on different ECM protein substrates, incubated for 24 hours and harvested using RIPA buffer. 7.5 µg of total protein was loaded per lane. Equal loading was verified by re-probing for beta -actin. The poloxamer pluronic was used as a no-adhesion control. Col I = Collagen I, Vn = Vitronectin, Fn = Fibronectin, Lm = Laminin, ECL = Entactin, Collagen, Laminin, and FBS = Foetal Bovine Serum. C: Cells cultured in LCM (LM) or serum free medium (SF) for 24 hours were harvested using sample buffer and deglycosylated by PNGase F treatment (+) as indicated. Re-probing for beta -actin was used as a loading control. The three bands detected by the anti-uPAR antibody are labelled 1, 2, and 3, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse uPAR by Immunohistochemistry View Larger

Detection of Mouse uPAR by Immunohistochemistry In vivo tongue tumours of EV1 and uPAR1 knock-down cells.IHC uPAR staining and growth pattern of tongue tumours generated from the EV1 and uPAR1 cells containing either shRNA targeting uPAR (EV1-sh and uPAR1-sh), or non-targeting shRNA (uPAR1-NT). A–C: IHC uPAR staining of EV1-sh (A), uPAR1-NT (B) and uPAR1-sh (C) tumours, respectively. Images were recorded at 20x magnifications. D: Representative image depicting the tumour growth pattern at the tumour-stroma interface in hematoxylin/eosin stained EV1-sh. E: The average SI of the uPAR staining in the tumours, with the maximum obtainable score of 9. The error bars shows the +SEM. N = number of tumours; EV1, N = 8/10; EV1-sh, N = 11/16; uPAR1, N = 4/10; uPAR1-NT, N = 3/8; uPAR1-sh, N = 4/16. One-way ANOVA; **p<0.01, *p<0.05. T = Tumour, S = Stroma. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse uPAR by Western Blot View Larger

Detection of Mouse uPAR by Western Blot Expression of murine Plaur in AT84 cells.In vitro characterization of AT84 cells stably transfected with either empty vector (EV) or a vector containing cDNA encoding murine uPAR (Plaur). A: Western blot analysis of whole cell lysates using a polyclonal anti-murine uPAR antibody (AF534). A total of 7.5 ng of recombinant murine uPAR (rmuPAR) was loaded as a positive control. Re-probing for beta -actin was used as a loading control. B: Western blot analysis of cellular membrane fractions using a polyclonal anti-murine uPAR antibody (AF534). Total protein was measured per sample and 53.5 µg of protein was loaded per lane. A and B: Images were cropped, as no additional bands were detectable. C: FACS analysis of non-permeabilized cells using a polyclonal anti-murine uPAR antibody (AF534). Alexa Fluor 488 anti-goat secondary antibody (A11055) was used as the secondary antibody. The quantified mean Alexa 488 fluorescence signal per cell line is presented in the panel to the right. D and E: Relative Plaur mRNA (uPAR) (D) or Plau mRNA (uPA) (E) expression levels as analysed using RT-qPCR. All expression levels were normalized to the expression of the reference genes Trfc and beta -actin. Error bars represent the standard error of mean (+SEM) and N = 3. One-way ANOVA; *p<0.05. F: Plasminogen-gelatin (upper panel) and gelatin (lower panel) zymography analysis of conditioned medium of cells cultured for 24 hours in SFM. HMW-uPA and mPLM (mouse plasmin) were loaded as positive controls. The images were cropped to size. G: Relative Plasminogen mRNA (Plg) expression levels as analysed using RT-qPCR. Error bars represent standard error of mean (+SEM) and N = 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25157856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: uPAR

The urokinase-type plasminogen activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single‑chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. Mouse uPAR-1/Fc cDNA encodes a 327 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide, seven potential N-linked glycosylation sites and a C-terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is highly species-specific. Human uPA binds rmuPAR at a lower affinity compared to rhuPAR.

References
  1. Dear, A.E. and R.L. Medcalf (1998) Eur. J. Biochemistry 252:185.
Long Name
Urokinase-type Plasminogen Activator Receptor
Entrez Gene IDs
5329 (Human); 18793 (Mouse); 102139334 (Cynomolgus Monkey)
Alternate Names
CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3

Product Datasheets

You must select a language.

x

Citations for Mouse uPAR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Viral anti-inflammatory serpin reduces immuno-coagulopathic pathology in SARS-CoV-2 mouse models of infection
    Authors: Zhang, L;Li, YH;Kibler, K;Kraberger, S;Varsani, A;Turk, J;Elmadbouly, N;Aliskevich, E;Spaccarelli, L;Estifanos, B;Enow, J;Zanetti, IR;Saldevar, N;Lim, E;Schlievert, J;Browder, K;Wilson, A;Juan, FA;Pinteric, A;Garg, A;Monder, H;Saju, R;Gisriel, S;Jacobs, B;Karr, TL;Florsheim, EB;Kumar, V;Wallen, J;Rahman, M;McFadden, G;Hogue, BG;Lucas, AR;
    EMBO molecular medicine
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. PEGylated Serp-1 Markedly Reduces Pristane-Induced Experimental Diffuse Alveolar Hemorrhage, Altering uPAR Distribution, and Macrophage Invasion
    Authors: Qiuyun Guo, Jordan R. Yaron, John W. Wallen, Kyle F. Browder, Ryan Boyd, Tien L. Olson et al.
    Frontiers in Cardiovascular Medicine
  3. In vivo antitumor activity by dual stromal and tumor-targeted oncolytic measles viruses
    Authors: Yuqi Jing, Valery Chavez, Natasha Khatwani, Yuguang Ban, Andrea P. Espejo, Xi Chen et al.
    Cancer Gene Therapy
  4. Senolytic CAR T cells reverse senescence-associated pathologies
    Authors: C Amor, J Feucht, J Leibold, YJ Ho, C Zhu, D Alonso-Cur, J Mansilla-S, JA Boyer, X Li, T Giavridis, A Kulick, S Houlihan, E Peerschke, SL Friedman, V Ponomarev, A Piersigill, M Sadelain, SW Lowe
    Nature, 2020-06-17;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Flow Cytometry
  5. Further Evidence That the Soluble Urokinase Plasminogen Activator Receptor Does Not Directly Injure Mice or Human Podocytes
    Authors: Efrat Harel, Jun Shoji, Vivek Abraham, Loan Miller, Zoltan G. Laszik, Andrew King et al.
    Transplantation
  6. Fibrinolysis protease receptors promote activation of astrocytes to express pro-inflammatory cytokines
    Authors: P Pontecorvi, MA Banki, C Zampieri, C Zalfa, P Azmoon, MZ Kounnas, C Marchese, SL Gonias, E Mantuano
    J Neuroinflammation, 2019-12-06;16(1):257.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  7. A Virus-Derived Immune Modulating Serpin Accelerates Wound Closure with Improved Collagen Remodeling
    Authors: L Zhang, JR Yaron, AM Tafoya, SE Wallace, J Kilbourne, S Haydel, K Rege, G McFadden, AR Lucas
    J Clin Med, 2019-10-04;8(10):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. uPAR isoform 2 forms a dimer and induces severe kidney disease in mice
    Authors: C Wei, J Li, BD Adair, K Zhu, J Cai, M Merchant, B Samelko, Z Liao, KH Koh, NJ Tardi, RR Dande, S Liu, J Ma, S DiBartolo, S Hägele, V Peev, SS Hayek, DJ Cimbaluk, M Tracy, JB Klein, S Sever, SJ Shattil, MA Arnaout, J Reiser
    J. Clin. Invest., 2019-04-02;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Cleavage of the urokinase receptor (uPAR) on oral cancer cells: regulation by transforming growth factor - ?1 (TGF-?1) and potential effects on migration and invasion
    Authors: SN Magnussen, E Hadler-Ols, DE Costea, E Berg, CC Jacobsen, B Mortensen, T Salo, I Martinez-Z, JO Winberg, L Uhlin-Hans, G Svineng
    BMC Cancer, 2017-05-19;17(1):350.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Urokinase-type Plasminogen Activator (uPA) Binding to the uPA Receptor (uPAR) Promotes Axonal Regeneration in the Central Nervous System
    Authors: Paola Merino
    J. Biol. Chem, 2016-12-16;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. A reassessment of soluble urokinase-type plasminogen activator receptor in glomerular disease.
    Authors: Spinale J, Mariani L, Kapoor S, Zhang J, Weyant R, Song P, Wong H, Troost J, Gadegbeku C, Gipson D, Kretzler M, Nihalani D, Holzman L
    Kidney Int, 2014-10-29;87(3):564-74.
    Species: Mouse
    Sample Types: Serum, Tissue Homogenates
    Applications: ELISA Development (Detection), Western Blot
  12. Tumour microenvironments induce expression of urokinase plasminogen activator receptor (uPAR) and concomitant activation of gelatinolytic enzymes.
    Authors: Magnussen S, Hadler-Olsen E, Latysheva N, Pirila E, Steigen S, Hanes R, Salo T, Winberg J, Uhlin-Hansen L, Svineng G
    PLoS ONE, 2014-08-26;9(8):e105929.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC, Western Blot
  13. Antipermeability function of PEDF involves blockade of the MAP kinase/GSK/beta-catenin signaling pathway and uPAR expression.
    Authors: Yang J, Duh EJ, Caldwell RB, Behzadian MA
    Invest. Ophthalmol. Vis. Sci., 2010-01-20;51(6):3273-80.
    Species: Bovine
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  14. Urokinase-type plasminogen activator receptor (uPAR) augments brain damage in a murine model of ischemic stroke.
    Authors: Nagai N, Okada K, Kawao N, Ishida C, Ueshima S, Collen D, Matsuo O
    Neurosci. Lett., 2007-12-08;432(1):46-9.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Immunoprecipitation
  15. Inhibition of choroidal neovascularization by a peptide inhibitor of the urokinase plasminogen activator and receptor system in a mouse model.
    Authors: Das A, Boyd N, Jones TR, Talarico N, McGuire PG
    Arch. Ophthalmol., 2004-12-01;122(12):1844-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  16. Mitogenic signaling of urokinase receptor-deficient kidney fibroblasts: actions of an alternative urokinase receptor and LDL receptor-related protein.
    Authors: Zhang G, Cai X, Lopez-Guisa JM, Collins SJ, Eddy AA
    J. Am. Soc. Nephrol., 2004-08-01;15(8):2090-102.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Neutralization, Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse uPAR Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Mouse uPAR Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse uPAR Antibody
By Anonymous on 01/13/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: bEnd.3 mouse endothelioma cell line Species: Mouse

No permeabilization with triton X-100
1% BSA, 30 min, RT
Primary Ab: 1 ug/mL, 1h, RT
Secondary Ab: AlexaFluor 448 donkey anti-goat IgG (H+L), 1:800, 30 min, RT