Mouse uPAR Antibody
Mouse uPAR Antibody Summary
Leu24-Thr297
Accession # Q545X5
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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uPAR in Mouse Kidney. uPAR was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF534) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Detection of uPAR in Raw264.7 cells by Flow Cytometry. Raw264.7 cells were stained with Goat Anti-Mouse uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF534, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: uPAR
The urokinase-type plasminogen activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single‑chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. Mouse uPAR-1/Fc cDNA encodes a 327 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide, seven potential N-linked glycosylation sites and a C-terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is highly species-specific. Human uPA binds rmuPAR at a lower affinity compared to rhuPAR.
Product Datasheets
Citations for Mouse uPAR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
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uPAR isoform 2 forms a dimer and induces severe kidney disease in mice
Authors: C Wei, J Li, BD Adair, K Zhu, J Cai, M Merchant, B Samelko, Z Liao, KH Koh, NJ Tardi, RR Dande, S Liu, J Ma, S DiBartolo, S Hägele, V Peev, SS Hayek, DJ Cimbaluk, M Tracy, JB Klein, S Sever, SJ Shattil, MA Arnaout, J Reiser
J. Clin. Invest., 2019;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
A Virus-Derived Immune Modulating Serpin Accelerates Wound Closure with Improved Collagen Remodeling
Authors: L Zhang, JR Yaron, AM Tafoya, SE Wallace, J Kilbourne, S Haydel, K Rege, G McFadden, AR Lucas
J Clin Med, 2019;8(10):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Fibrinolysis protease receptors promote activation of astrocytes to express pro-inflammatory cytokines
Authors: P Pontecorvi, MA Banki, C Zampieri, C Zalfa, P Azmoon, MZ Kounnas, C Marchese, SL Gonias, E Mantuano
J Neuroinflammation, 2019;16(1):257.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Cleavage of the urokinase receptor (uPAR) on oral cancer cells: regulation by transforming growth factor - ?1 (TGF-?1) and potential effects on migration and invasion
Authors: SN Magnussen, E Hadler-Ols, DE Costea, E Berg, CC Jacobsen, B Mortensen, T Salo, I Martinez-Z, JO Winberg, L Uhlin-Hans, G Svineng
BMC Cancer, 2017;17(1):350.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Urokinase-type Plasminogen Activator (uPA) Binding to the uPA Receptor (uPAR) Promotes Axonal Regeneration in the Central Nervous System
Authors: Paola Merino
J. Biol. Chem, 2016;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
A reassessment of soluble urokinase-type plasminogen activator receptor in glomerular disease.
Authors: Spinale J, Mariani L, Kapoor S, Zhang J, Weyant R, Song P, Wong H, Troost J, Gadegbeku C, Gipson D, Kretzler M, Nihalani D, Holzman L
Kidney Int, 2015;87(3):564-74.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development (Detection) -
Tumour microenvironments induce expression of urokinase plasminogen activator receptor (uPAR) and concomitant activation of gelatinolytic enzymes.
Authors: Magnussen S, Hadler-Olsen E, Latysheva N, Pirila E, Steigen S, Hanes R, Salo T, Winberg J, Uhlin-Hansen L, Svineng G
PLoS ONE, 2014;9(8):e105929.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Antipermeability function of PEDF involves blockade of the MAP kinase/GSK/beta-catenin signaling pathway and uPAR expression.
Authors: Yang J, Duh EJ, Caldwell RB, Behzadian MA
Invest. Ophthalmol. Vis. Sci., 2010;51(6):3273-80.
Species: Bovine
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Urokinase-type plasminogen activator receptor (uPAR) augments brain damage in a murine model of ischemic stroke.
Authors: Nagai N, Okada K, Kawao N, Ishida C, Ueshima S, Collen D, Matsuo O
Neurosci. Lett., 2007;432(1):46-9.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Immunoprecipitation -
Inhibition of choroidal neovascularization by a peptide inhibitor of the urokinase plasminogen activator and receptor system in a mouse model.
Authors: Das A, Boyd N, Jones TR, Talarico N, McGuire PG
Arch. Ophthalmol., 2004;122(12):1844-9.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Mitogenic signaling of urokinase receptor-deficient kidney fibroblasts: actions of an alternative urokinase receptor and LDL receptor-related protein.
Authors: Zhang G, Cai X, Lopez-Guisa JM, Collins SJ, Eddy AA
J. Am. Soc. Nephrol., 2004;15(8):2090-102.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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No permeabilization with triton X-100
1% BSA, 30 min, RT
Primary Ab: 1 ug/mL, 1h, RT
Secondary Ab: AlexaFluor 448 donkey anti-goat IgG (H+L), 1:800, 30 min, RT
