Mouse uPAR Antibody

Name: Mouse uPAR Antibody
Brand: R&D Systems

(8 citations)

Datasheet / CoA / SDS

Immunohistochemistry

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Product Details

Citations (8)

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Species Reactivity

Mouse
Specificity

Detects mouse uPAR in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human uPAR is observed.
Source

Polyclonal Goat IgG
Purification

Antigen Affinity-purified
Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse uPAR isoform 1
Leu24-Thr297
Accession # Q545X5
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse uPAR Fc Chimera (Catalog # 531-PA)
Flow Cytometry

2.5 µg/10⁶ cells

Mouse whole blood
Immunohistochemistry

5-15 µg/mL

See below
Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.8-4 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human u-Plasminogen Activator/Urokinase (Catalog # 1310-SE) to immobilized Recombinant Mouse uPAR Fc Chimera (Catalog # 531-PA) coated at 5 µg/mL (100 µL/well). At 25 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry

click image to view larger

uPAR in Mouse Kidney. uPAR was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF534) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: uPAR

The urokinase-type plasminogen activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single‑chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. Mouse uPAR-1/Fc cDNA encodes a 327 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide, seven potential N-linked glycosylation sites and a C-terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is highly species-specific. Human uPA binds rmuPAR at a lower affinity compared to rhuPAR.

References:
1. Dear, A.E. and R.L. Medcalf (1998) Eur. J. Biochemistry 252:185.

Long Name:

Urokinase-type Plasminogen Activator Receptor

Entrez Gene IDs:

5329 (Human); 18793 (Mouse)

Alternate Names:

CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species	Applications	Sample Type
Cleavage of the urokinase receptor (uPAR) on oral cancer cells: regulation by transforming growth factor - ?1 (TGF-?1) and potential effects on migration and invasion Authors: SN Magnussen, E Hadler-Ols, DE Costea, E Berg, CC Jacobsen, B Mortensen, T Salo, I Martinez-Z, JO Winberg, L Uhlin-Hans, G Svineng BMC Cancer, 2017;17(1):350. Species: Mouse Sample Type: Whole Cells Application: Flow Urokinase-type Plasminogen Activator (uPA) Binding to the uPA Receptor (uPAR) Promotes Axonal Regeneration in the Central Nervous System Authors: Paola Merino J. Biol. Chem, 2016;0(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC A reassessment of soluble urokinase-type plasminogen activator receptor in glomerular disease. Authors: Spinale J, Mariani L, Kapoor S, Zhang J, Weyant R, Song P, Wong H, Troost J, Gadegbeku C, Gipson D, Kretzler M, Nihalani D, Holzman L Kidney Int, 2015;87(3):564-74. Species: Mouse Sample Type: Serum Application: ELISA detection Tumour microenvironments induce expression of urokinase plasminogen activator receptor (uPAR) and concomitant activation of gelatinolytic enzymes. Authors: Magnussen S, Hadler-Olsen E, Latysheva N, Pirila E, Steigen S, Hanes R, Salo T, Winberg J, Uhlin-Hansen L, Svineng G PLoS ONE, 2014;9(8):e105929. Species: Mouse Sample Type: Cell Lysates Application: WB Antipermeability function of PEDF involves blockade of the MAP kinase/GSK/beta-catenin signaling pathway and uPAR expression. Authors: Yang J, Duh EJ, Caldwell RB, Behzadian MA Invest. Ophthalmol. Vis. Sci., 2010;51(6):3273-80. Species: Bovine Sample Type: Cell Culture Supernates Application: WB Urokinase-type plasminogen activator receptor (uPAR) augments brain damage in a murine model of ischemic stroke. Authors: Nagai N, Okada K, Kawao N, Ishida C, Ueshima S, Collen D, Matsuo O Neurosci. Lett., 2007;432(1):46-9. Species: Mouse Sample Type: Tissue Homogenates Application: IP Inhibition of choroidal neovascularization by a peptide inhibitor of the urokinase plasminogen activator and receptor system in a mouse model. Authors: Das A, Boyd N, Jones TR, Talarico N, McGuire PG Arch. Ophthalmol., 2004;122(12):1844-9. Species: Mouse Sample Type: Whole Cells Application: ICC Mitogenic signaling of urokinase receptor-deficient kidney fibroblasts: actions of an alternative urokinase receptor and LDL receptor-related protein. Authors: Zhang G, Cai X, Lopez-Guisa JM, Collins SJ, Eddy AA J. Am. Soc. Nephrol., 2004;15(8):2090-102. Species: Mouse Sample Type: Cell Lysates Application: WB

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Cell and Tissue Staining Kits

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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 17

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Mouse uPAR Antibody

Certificate of Analysis Lookup

Certificate of Analysis Lookup

Certificate of Analysis Request Form

FAQs

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

Antigen Retrieval Reagent-Universal

Antigen Retrieval Reagent-Basic

Mounting Medium

Antigen Retrieval Reagent Sampler (50 mL each)

Antigen Retrieval Reagent-Acidic

VisUCyte Antigen Retrieval Reagent-Universal

VisUCyte Antigen Retrieval Reagent-Acidic

VisUCyte Antigen Retrieval Reagent-Basic

NorthernLights Guard Mounting Media

Normal Goat IgG Control

Reconstitution Buffer 1 (PBS)

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