Mouse uPAR Antibody

Catalog # Availability Size / Price Qty
AF534
AF534-SP
uPAR in Mouse Kidney.
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Product Details
Citations (11)
FAQs
Supplemental Products
Reviews (1)

Mouse uPAR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse uPAR in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse uPAR isoform 1
Leu24-Thr297
Accession # Q545X5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse uPAR Fc Chimera (Catalog # 531-PA)
Flow Cytometry
0.25 µg/106 cells
RAW 264.7 mouse monocyte/macrophage cell line
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.8-4 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human u-Plasminogen Activator/Urokinase (Catalog # 1310-SE) to immobilized Recombinant Mouse uPAR Fc Chimera (Catalog # 531-PA) coated at 5 µg/mL (100 µL/well). At 25 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry uPAR antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

uPAR in Mouse Kidney. uPAR was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF534) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Flow Cytometry View Larger

Detection of uPAR in Raw264.7 cells by Flow Cytometry. Raw264.7 cells were stained with Goat Anti-Mouse uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF534, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: uPAR

The urokinase-type plasminogen activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single‑chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. Mouse uPAR-1/Fc cDNA encodes a 327 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide, seven potential N-linked glycosylation sites and a C-terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is highly species-specific. Human uPA binds rmuPAR at a lower affinity compared to rhuPAR.

References
  1. Dear, A.E. and R.L. Medcalf (1998) Eur. J. Biochemistry 252:185.
Long Name
Urokinase-type Plasminogen Activator Receptor
Entrez Gene IDs
5329 (Human); 18793 (Mouse); 102139334 (Cynomolgus Monkey)
Alternate Names
CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3

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Citations for Mouse uPAR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. uPAR isoform 2 forms a dimer and induces severe kidney disease in mice
    Authors: C Wei, J Li, BD Adair, K Zhu, J Cai, M Merchant, B Samelko, Z Liao, KH Koh, NJ Tardi, RR Dande, S Liu, J Ma, S DiBartolo, S Hägele, V Peev, SS Hayek, DJ Cimbaluk, M Tracy, JB Klein, S Sever, SJ Shattil, MA Arnaout, J Reiser
    J. Clin. Invest., 2019;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. A Virus-Derived Immune Modulating Serpin Accelerates Wound Closure with Improved Collagen Remodeling
    Authors: L Zhang, JR Yaron, AM Tafoya, SE Wallace, J Kilbourne, S Haydel, K Rege, G McFadden, AR Lucas
    J Clin Med, 2019;8(10):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Fibrinolysis protease receptors promote activation of astrocytes to express pro-inflammatory cytokines
    Authors: P Pontecorvi, MA Banki, C Zampieri, C Zalfa, P Azmoon, MZ Kounnas, C Marchese, SL Gonias, E Mantuano
    J Neuroinflammation, 2019;16(1):257.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Cleavage of the urokinase receptor (uPAR) on oral cancer cells: regulation by transforming growth factor - ?1 (TGF-?1) and potential effects on migration and invasion
    Authors: SN Magnussen, E Hadler-Ols, DE Costea, E Berg, CC Jacobsen, B Mortensen, T Salo, I Martinez-Z, JO Winberg, L Uhlin-Hans, G Svineng
    BMC Cancer, 2017;17(1):350.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Urokinase-type Plasminogen Activator (uPA) Binding to the uPA Receptor (uPAR) Promotes Axonal Regeneration in the Central Nervous System
    Authors: Paola Merino
    J. Biol. Chem, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. A reassessment of soluble urokinase-type plasminogen activator receptor in glomerular disease.
    Authors: Spinale J, Mariani L, Kapoor S, Zhang J, Weyant R, Song P, Wong H, Troost J, Gadegbeku C, Gipson D, Kretzler M, Nihalani D, Holzman L
    Kidney Int, 2015;87(3):564-74.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  7. Tumour microenvironments induce expression of urokinase plasminogen activator receptor (uPAR) and concomitant activation of gelatinolytic enzymes.
    Authors: Magnussen S, Hadler-Olsen E, Latysheva N, Pirila E, Steigen S, Hanes R, Salo T, Winberg J, Uhlin-Hansen L, Svineng G
    PLoS ONE, 2014;9(8):e105929.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Antipermeability function of PEDF involves blockade of the MAP kinase/GSK/beta-catenin signaling pathway and uPAR expression.
    Authors: Yang J, Duh EJ, Caldwell RB, Behzadian MA
    Invest. Ophthalmol. Vis. Sci., 2010;51(6):3273-80.
    Species: Bovine
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  9. Urokinase-type plasminogen activator receptor (uPAR) augments brain damage in a murine model of ischemic stroke.
    Authors: Nagai N, Okada K, Kawao N, Ishida C, Ueshima S, Collen D, Matsuo O
    Neurosci. Lett., 2007;432(1):46-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  10. Inhibition of choroidal neovascularization by a peptide inhibitor of the urokinase plasminogen activator and receptor system in a mouse model.
    Authors: Das A, Boyd N, Jones TR, Talarico N, McGuire PG
    Arch. Ophthalmol., 2004;122(12):1844-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  11. Mitogenic signaling of urokinase receptor-deficient kidney fibroblasts: actions of an alternative urokinase receptor and LDL receptor-related protein.
    Authors: Zhang G, Cai X, Lopez-Guisa JM, Collins SJ, Eddy AA
    J. Am. Soc. Nephrol., 2004;15(8):2090-102.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Mouse uPAR Antibody
By Anonymous on 01/13/2022
Application: ICC/IF Sample Tested: bEnd.3 mouse endothelioma cell line Species: Mouse

No permeabilization with triton X-100
1% BSA, 30 min, RT
Primary Ab: 1 ug/mL, 1h, RT
Secondary Ab: AlexaFluor 448 donkey anti-goat IgG (H+L), 1:800, 30 min, RT