Detection of Urotensin-II R in HEK293 Human Cell Line Transfected with Mouse Urotensin-II and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) mouse Urotensin-II R or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse Urotensin-II R Monoclonal Antibody (Catalog # MAB9245) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB0061). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Urotensin-II R
Urotensin-II R (UR2R)
belongs to the G protein-coupled receptor 1 family. UR2R
is a high affinity receptor for urotensin-2 and urotensin-2B. The
activity of UR2R is mediated by a G protein that activates a
phosphatidylinositol-calcium second messenger system. It is most abundantly
expressed in the heart and pancreas. Human UR2R has been correlated with
cortico-adrenal carcinoma proliferation.
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