Mouse Urotensin-II R Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Urotensin-II R.  Stains mouse Urotensin-II R transfectants but not irrelevant transfectants in flow cytometry.
  • Source
    Monoclonal Rat IgG2B Clone # 443707
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse Urotensin-II R synthetic peptide
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Urotensin-II R in HEK293 Human Cell Line Transfected with Mouse Urotensin-II and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse Urotensin-II R or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse Urotensin-II R Monoclonal Antibody (Catalog # MAB9245) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB0061). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Urotensin-II R
Urotensin-II R (UR2R) belongs to the G protein-coupled receptor 1 family. UR2R is a high affinity receptor for urotensin-2 and urotensin-2B. The activity of UR2R is mediated by a G protein that activates a phosphatidylinositol-calcium second messenger system. It is most abundantly expressed in the heart and pancreas. Human UR2R has been correlated with cortico-adrenal carcinoma proliferation.
  • Long Name:
    Urotensin-II Receptor
  • Entrez Gene IDs:
    2837 (Human); 217369 (Mouse); 57305 (Rat)
  • Alternate Names:
    G protein-coupled receptor 14; GPR14; G-protein coupled receptor 14; UR-2-R; UR-II-R; urotensin 2 receptor; urotensin II receptor; urotensin-2 receptor; Urotensin-II R; UrotensinIIR; Urotensin-IIR; UTR; UTR2; UTS2R
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Isotype Control

Ctrl MAB0061 27  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2B Isotype Control

Ctrl MAB0061R
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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