Mouse VEGF120/164 Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (9)
Supplemental Products

Mouse VEGF120/164 Antibody Summary

Species Reactivity
Detects mouse VEGF120/164 in direct ELISAs and Western blots. This antibody does not recognize recombinant mouse VEGF115 or any isoform of human VEGF.
Monoclonal Rat IgG2B Clone # 39917
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGF164
Accession # AAA40547
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Mouse VEGF120 (Catalog # 494-VE)
Recombinant Mouse VEGF164 (Catalog # 493-MV)
under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF164 appears to be the most abundant and potent isoform, followed by VEGF120 and VEGF188. Mouse VEGF164 is an approximately 50 kDa molecular weight homodimer sharing 97% aa sequence identity with corresponding regions of rat, 89% with human and porcine, 90% with feline, equine and canine, and 88% with bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. Human VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Long Name
Vascular Endothelial Growth Factor
Entrez Gene IDs
7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

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Citations for Mouse VEGF120/164 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Direct thrombin inhibitors as alternatives to heparin to preserve lung growth and function in a murine model of compensatory lung growth
    Authors: ST Tsikis, TI Hirsch, SC Fligor, A Pan, MM Joiner, A Devietro, PD Mitchell, H Kishikawa, KM Gura, M Puder
    Scientific Reports, 2022;12(1):21117.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  2. Lipopolysaccharide-induced murine lung injury results in long-term pulmonary changes and downregulation of angiogenic pathways
    Authors: ST Tsikis, SC Fligor, TI Hirsch, A Pan, LJ Yu, H Kishikawa, MM Joiner, PD Mitchell, M Puder
    Oncogene, 2022;12(1):10245.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Temporally distinct myeloid cell responses mediate damage and repair after cerebrovascular injury
    Authors: P Mastorakos, N Mihelson, M Luby, SR Burks, K Johnson, AW Hsia, J Witko, JA Frank, L Latour, DB McGavern
    Nature Neuroscience, 2021;24(2):245-258.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Investigation of the mechanisms of VEGF-mediated compensatory lung growth: the role of the VEGF heparin-binding domain
    Authors: LJ Yu, VH Ko, DT Dao, JD Secor, A Pan, BS Cho, PD Mitchell, H Kishikawa, DR Bielenberg, M Puder
    Scientific Reports, 2021;11(1):11827.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. A paradoxical method to enhance compensatory lung growth: Utilizing a VEGF inhibitor
    Authors: DT Dao, L Anez-Busti, SS Jabbouri, A Pan, H Kishikawa, PD Mitchell, GL Fell, MA Baker, RS Watnick, H Chen, MS Rogers, DR Bielenberg, M Puder
    PLoS ONE, 2018;13(12):e0208579.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. The Novel Mechanisms Concerning the Inhibitions of Palmitate-Induced Proinflammatory Factor Releases and Endogenous Cellular Stress with Astaxanthin on MIN6 ?-Cells
    Authors: A Kitahara, K Takahashi, N Morita, T Murashima, H Onuma, Y Sumitani, T Tanaka, T Kondo, T Hosaka, H Ishida
    Mar Drugs, 2017;15(6):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. VEGF-production by CCR2-dependent macrophages contributes to laser-induced choroidal neovascularization.
    Authors: Krause T, Alex A, Engel D, Kurts C, Eter N
    PLoS ONE, 2014;9(4):e94313.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  8. Suppression of laser-induced choroidal neovascularization by a CCR3 antagonist.
    Authors: Mizutani, Takeshi, Ashikari, Masayuki, Tokoro, Mayumi, Nozaki, Miho, Ogura, Yuichiro
    Invest Ophthalmol Vis Sci, 2013;54(2):1564-72.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Pericytes promote endothelial cell survival through induction of autocrine VEGF-A signaling and Bcl-w expression.
    Authors: Franco M, Roswall P, Cortez E, Hanahan D, Pietras K
    Blood, 2011;118(10):2906-17.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


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