Mouse VEGF-D Antibody

Catalog # Availability Size / Price Qty
AF469
AF469-SP

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VEGF-D in Mouse Lung Tissue.
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Product Details
Citations (5)
FAQs
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Reviews (1)

Mouse VEGF-D Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse VEGF-D in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant human (rh) VEGF-D is observed, and less than 1% cross-reactivity with recombinant mouse (rm) VEGF115, rmVEGF120, rhVEGF, rhVEGF-B, and rmVEGF165 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse VEGF-D
Phe98-Ser206
Accession # P97946
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse VEGF-D (Catalog # 469-VD)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry VEGF-D in Mouse Lung Tissue. View Larger

VEGF-D in Mouse Lung Tissue. VEGF-D was detected in immersion fixed paraffin-embedded sections of mouse lung tissue using Goat Anti-Mouse VEGF-D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF469) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in pneumocytes in lung. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF-D

Vascular endothelia growth factor D (VEGF-D), also known as c-fos-induced growth factor (FIGF), is a secreted glycoprotein of the VEGF/PDGF family. VEGFs regulate angiogenesis and lymphangiogenesis during development and tumor growth, and are characterized by eight conserved cysteine residues that form a cysteine-knot structure (1‑3). VEGF-C and VEGF-D, which share 23% amino acid (aa) sequence identity, are uniquely expressed as preproproteins that contain long N- and C-terminal propeptide extensions around the VEGF homology domain (VHD) (1, 2). Proteolytic processing of either 358 aa or 326 aa splice forms of mouse VEGF-D preproprotein creates a secreted proprotein. Further processing by extracellular serine proteases, such as plasmin or furin-like proprotein convertases, forms mature VEGF-D consisting of non-covalently linked 42 kDa homodimers of the 117 aa VHD (4‑7). Mature mouse VEGF-D shares 94%, 99%, 93%, 91% and 89% aa identity with the VHD of human, rat, equine, canine and bovine VEGF-D, respectively. It is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin (1-4). Mouse and human VEGF-D are ligands for VEGF receptor 3 (VEGF-R3, also called Flt-4) that are active across species and show enhanced affinity when processed (8). Unlike human VEGF‑D, which is also a ligand for VEGF-R2 (also called Flk-1 or KDR), mouse VEGF-D does not bind to VEGF-R2 (8). VEGF-R3 is strongly expressed in lymphatic endothelial cells and is essential for regulation of the growth and differentiation of lymphatic endothelium (1, 2). While VEGF-C is the critical ligand for VEGF-R3 during embryonic lymphatic development, VEGF-D is most active in neonatal lymphatic maturation and bone growth (9‑11). Both promote tumor lymphangiogenesis (12). Consonant with their activity on VEGF receptors, binding of VEGF-C and VEGF-D to neuropilins contributes to VEGF-R3 signaling in lymphangiogenesis, while binding to integrin alpha 9 beta 1 mediates endothelial cell adhesion and migration (13, 14).

References
  1. Roy, H. et al. (2006) FEBS Lett. 580:2879.
  2. Otrock, Z.H. et al. (2007) Blood Cells Mol. Dis. 38:258.
  3. Orlandini, M. et al. (1996) Proc. Natl. Acad. Sci. USA 93:11675.
  4. Stacker, S.A. et al. (1999) J. Biol. Chem. 274:32127.
  5. McColl, B.K. et al. (2003) J. Exp. Med. 198:863.
  6. McColl, B.K. et al. (2007) FASEB J. 21:1088.
  7. Baldwin, M.E. et al. (2001) J. Biol. Chem. 276:44307.
  8. Baldwin, M.E. et al. (2001) J. Biol. Chem. 276:19166.
  9. Baldwin, M.E. et al. (2005) Mol. Cell. Biol. 25:2441.
  10. Karpanen, T. et al. (2006) Am. J. Pathol. 169:708.
  11. Orlandini, M. et al. (2006) J. Biol. Chem. 281:17961.
  12. Stacker, S.A. et al. (2001) Nature Med. 7:186.
  13. Karpanen, T. et al. (2006) FASEB J. 20:1462.
  14. Vlahakis, N.E. et al. (2005) J. Biol. Chem. 280:4544.
Long Name
Vascular Endothelial Growth Factor D/cFos-induced Growth Factor
Entrez Gene IDs
2277 (Human); 14205 (Mouse)
Alternate Names
c-fos induced growth factor (vascular endothelial growth factor D); FIGF; vascular endothelial growth factor D; VEGFD; VEGF-D; VEGF-DVEGFDc-Fos-induced growth factor

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Citations for Mouse VEGF-D Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Inhibition of receptor activity-modifying protein 1 suppresses the development of endometriosis and the formation of blood and lymphatic vessels
    Authors: M Honda, Y Ito, K Hattori, K Hosono, K Sekiguchi, K Tsujikawa, N Unno, M Majima
    J. Cell. Mol. Med., 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Dilated thin-walled blood and lymphatic vessels in human endometrium: a potential role for VEGF-D in progestin-induced break-through bleeding.
    Authors: Donoghue JF, McGavigan CJ, Lederman FL
    PLoS ONE, 2012;7(2):e30916.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Liposomal honokiol inhibits VEGF-D-induced lymphangiogenesis and metastasis in xenograft tumor model.
    Authors: Wen J, Fu AF, Chen LJ, Xie XJ, Yang GL, Chen XC, Wang YS, Li J, Chen P, Tang MH, Shao XM, Lu Y, Zhao X, Wei YQ
    Int. J. Cancer, 2009;124(11):2709-18.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  4. Effects of acute exercise, exercise training, and diabetes on the expression of lymphangiogenic growth factors and lymphatic vessels in skeletal muscle.
    Authors: Kivela R, Silvennoinen M, Lehti M, Kainulainen H, Vihko V
    Am. J. Physiol. Heart Circ. Physiol., 2007;293(4):H2573-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain.
    Authors: Le Bras B, Barallobre MJ, Homman-Ludiye J, Ny A, Wyns S, Tammela T, Haiko P, Karkkainen MJ, Yuan L, Muriel MP, Chatzopoulou E, Breant C, Zalc B, Carmeliet P, Alitalo K, Eichmann A, Thomas JL
    Nat. Neurosci., 2006;9(3):340-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Mouse VEGF-D Antibody
By Anonymous on 03/13/2020
Application: WB Sample Tested: Adult heart Species: Mouse

Dilution used was 1:300 in 3% Milk /TBS-T solution with overnight incubation at 4 degrees. Anti-Goat HRP 1:500 was used as secondary antibody (1h at room temperature)