Mouse VEGFR2/KDR/Flk-1 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of VEGF R2/KDR/Flk‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (3)
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Mouse VEGFR2/KDR/Flk-1 PE-conjugated Antibody Summary

Species Reactivity
Detects mouse VEGF R2/KDR/Flk‑1 in flow cytometry.
Monoclonal Rat IgG1 Clone # 522302
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse VEGF R2/KDR/Flk‑1
Accession # P35918
Supplied in a saline solution containing BSA and Sodium Azide.
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)


Recommended Concentration
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of VEGF R2/KDR/Flk-1 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of VEGF R2/KDR/Flk‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse VEGF R2/KDR/Flk-1 PE-conjugated Monoclonal Antibody (Catalog # FAB4432P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: VEGFR2/KDR/Flk-1

VEGF R2, also known as KDR, Flk-1, and CD309, is a 210-220 kDa type I transmembrane glycoprotein member of the 7-Ig/Class V subfamily that belongs to the tyrosine protein kinase family of molecules (1, 2). It is principally expressed on endothelial cells (EC), but also appears on neurons, mast cells, immature dendritic cells and monocytes (3-6). VEGF R2 serves as the major proangiogenic switch that regulates blood vessel development and repair (1, 2). It is known to bind VEGF-A, -C, and -D. On the cell surface, VEGF R2 either homodimerizes or heterodimerizes with VEGF R3 (1). As a homodimer, it appears to interact with Neuropilin-1 (7). It also interacts with Integrin beta 3 subunit, either through its extracellular domain or cytoplasmic tail (8). This interaction may be necessary for EC activation. Notably, VEGF R2 can also be activated in the absence of a VEGF ligand when under torsion stress and in a complex with CD31 and VE-Cadherin (9). Over amino acids 20-762, mouse VEGF R2 shares 93% and 80% amino acid sequence identity with rat and human VEGF R2, respectively.

  1. Smith, G.A. et al. (2015) Biosci. Rep. Aug 18 [ePub ahead of print].
  2. Shibuya, M. (2013) J. Biochem. 153:13.
  3. Le, X-F. et al. (2008) Cell Cycle 7:3747.
  4. Okabe, K. et al. (2014) Cell 159:584.
  5. Detoraki, A. et al. (2009) J. Allergy Clin. Immunol. 123:1142.
  6. Dong, Z. et al. (2009) Curr. Pharm. Des. 15:365.
  7. Plein, A. et al. (2014) Microcirculation 21:315.
  8. Somanath, P.R. et al. (2009) Angiogenesis 12:177.
  9. DuToit, A. (2015) Nat. Rev. Mol. Cell Biol. 16:268.
Long Name
Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs
3791 (Human); 16542 (Mouse)
Alternate Names
CD309 antigen; CD309; EC 2.7.10; EC; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2

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Citations for Mouse VEGFR2/KDR/Flk-1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Overexpression of the ?2AR gene improves function and re-endothelialization capacity of EPCs after arterial injury in nude mice
    Stem Cell Res Ther, 2016;7(1):73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Chemokine receptor CX3CR1 contributes to macrophage survival in tumor metastasis.
    Authors: Zheng, Jiao, Yang, Min, Shao, Jianghua, Miao, Yanju, Han, Jiahuai, Du, Jie
    Mol Cancer, 2013;12(1):141.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Expression of VEGF receptors on endothelial cells in mouse skeletal muscle.
    Authors: Imoukhuede, Princess, Popel, Aleksand
    PLoS ONE, 2012;7(9):e44791.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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