Mouse VEGFR2/KDR/Flk-1 Antibody Summary
Accession # P35918
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of VEGFR2/KDR/Flk‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endo-thelioma cell line was stained with Rat Anti-Mouse VEGFR2/KDR/Flk-1 Mono-clonal Antibody (Catalog # MAB4432, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113).
VEGFR2/KDR/Flk‑1 in bEnd.3 Mouse Cell Line. VEGFR2/KDR/Flk-1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using 10 µg/mL Rat Anti-Mouse VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB4432) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature mouse VEGFR2 is composed of a 743 amino acid (aa) extracellular domain, a 22 aa transmembrane domain, and a 583 aa cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity.
- Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
- Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
Citation for Mouse VEGFR2/KDR/Flk-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
M2-polarized macrophages contribute to neovasculogenesis, leading to relapse of oral cancer following radiation
Authors: Makiko Okubo
Sci Rep, 2016;6(0):27548.
Sample Types: Whole Tissue
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