Key Product Details

Species Reactivity

Mouse

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 368011
View all VG5Q Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse VG5Q
Met1-Glu711
Accession # Q7TN31

Specificity

Detects mouse VG5Q in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse VG5Q Antibody

VG5Q antibody in bEnd by Immunocytochemistry (ICC).3 Mouse Cell Line by Immunocytochemistry (ICC).

VG5Q in bEnd.3 Mouse Cell Line.

VG5Q was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse VG5Q Monoclonal Antibody (Catalog # MAB30481) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse VG5Q Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed bEND.3 mouse endothelioma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VG5Q

Angiogenic Factor VG5Q, also known as AGGF1, is a presumably secreted protein expressed by vascular endothelial cells. Mouse VG5Q cDNA encodes a 714 aa precursor with a coiled coil domain, a forkhead (FHA) domain, and RNA-association domains (G-patch, OCRE). VG5Q binds the angiogenic TNF superfamily ligand TWEAK, and promotes endothelial cell proliferation. Human VG5Q mutations are associated with Klippel-Trenaunay syndrome, a congenital vascular morphogenesis disorder. Mouse VG5Q shares 78% aa identity with human VG5Q.

Long Name

Vasculogenesis Gene on 5q/Angiogenic Factor with G Patch and FHA Domains 1

Alternate Names

AGGF1

Entrez Gene IDs

55109 (Human)

Gene Symbol

AGGF1

UniProt

Q7TN31

Additional VG5Q Products

Product Documents for Mouse VG5Q Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse VG5Q Antibody

For research use only

Related Research Areas

Endothelial Cell Markers

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