Mouse VG5Q Antibody

Catalog # Availability Size / Price Qty
MAB30481
MAB30481-SP

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VG5Q in bEnd.3 Mouse Cell Line.
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Mouse VG5Q Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse VG5Q in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 368011
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse VG5Q
Met1-Glu711
Accession # Q7TN31
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry VG5Q antibody in bEnd by Immunocytochemistry (ICC).3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

VG5Q in bEnd.3 Mouse Cell Line. VG5Q was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse VG5Q Monoclonal Antibody (Catalog # MAB30481) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VG5Q

Angiogenic Factor VG5Q, also known as AGGF1, is a presumably secreted protein expressed by vascular endothelial cells. Mouse VG5Q cDNA encodes a 714 aa precursor with a coiled coil domain, a forkhead (FHA) domain, and RNA-association domains (G-patch, OCRE). VG5Q binds the angiogenic TNF superfamily ligand TWEAK, and promotes endothelial cell proliferation. Human VG5Q mutations are associated with Klippel-Trenaunay syndrome, a congenital vascular morphogenesis disorder. Mouse VG5Q shares 78% aa identity with human VG5Q.

Long Name
Vasculogenesis Gene on 5q/Angiogenic Factor with G Patch and FHA Domains 1
Entrez Gene IDs
55109 (Human)
Alternate Names
AGGF1; Angiogenic factor VG5Q; angiogenic factor with G patch and FHA domains 1; FLJ10283hVG5Q; GPATC7G patch domain-containing protein 7; GPATCH7HUS84971; HSU84971; VG5Q; VG5QVasculogenesis gene on 5q protein

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