Mouse Vitamin D BP Antibody Summary
Accession # P21614
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse Vitamin D BP by Simple WesternTM. Simple Western lane view shows lysates of mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Vitamin D BP at approximately 58 kDa (as indicated) using 5 µg/mL of Sheep Anti-Mouse Vitamin D BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4188) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vitamin D BP
VDBP (Vitamin D binding protein; also group-specific component and GC-globulin) is a 52‑58 kDa, monomeric glycoprotein member of the ALB/AFP/VDB family of molecules. It is found in blood, urine and CSF, carries Vitamin D and its metabolites, and serves as an actin-scavenging protein. Mature mouse VDBP is 460 amino acids (aa) in length. It contains three albumin-type domains (aa 26‑476) that are accompanied by 14 intrachain disulfide bonds. There are three potential alternative splice forms. One shows a deletion of aa 346‑421, a second shows a 67 aa substitution for aa 345‑421, and a third shows a 34 aa substitution for aa 346‑423. All these variants involve the second and third albumin-like domains. Mature mouse VDBP (aa 17‑476) is 77% and 90% aa identical to human and rat VDBP, respectively.
Citation for Mouse Vitamin D BP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Nanoparticle-based targeted delivery of pentagalloyl glucose reverses elastase-induced abdominal aortic aneurysm and restores aorta to the healthy state in mice
Authors: S Dhital, NR Vyavahare
PLoS ONE, 2020;15(3):e0227165.
Sample Types: Whole Tissue
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