Mouse VLDL R Antibody

(7 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse VLDL R in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse VLDL R
    Thr25-Ala798
    Accession # AAA59384
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse VLDL R (Catalog # 2258-VL)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
VLDL R in Mouse Embryo. VLDL R was detected in immersion fixed frozen sections of mouse embryo using Mouse VLDL R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2258) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
VLDL R in Mouse Embryo. VLDL R was detected in immersion fixed frozen sections of mouse embryo using Mouse VLDL R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2258) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VLDL R

VLDL R is a 105 kDa type I integral membrane protein that belongs to the LDL receptor family. It plays a significant role in lipid metabolism and in nervous system development and function (1, 2). Mouse VLDL R has a 770 amino acid (aa) extracellular domain (ECD) and a 54 aa cytoplasmic region. The ECD contains eight LDLR class A repeats, three EGF-like repeats, six LDLR class B repeats, and a juxtamembrane region that is rich in O-linked glycosylation (3, 4). The cytoplasmic domain contains one NPXY internalization motif. VLDL R is predominantly expressed in striated muscle, adipose tissue, brain, and endothelial cells lining capillaries and small arterioles (3-6). VLDL R participates in the tissue uptake of fatty acids from plasma by mediating the internalization of ApoE-containing lipoparticles (i.e. VLDL, beta ‑VLDL, and chylomicron remnants) (5, 7). VLDL R binds and internalizes lipoprotein lipase (LPL) and mediates its transport from the basolateral to the lumenal face of endothelial cells (6, 8). VLDL R knockout mice are characterized by reduced LPL activity, reduced serum triglyceride clearance, and a resistance to developing obesity (7, 9, 10). VLDL R influences breast cancer cell motility by mediating the uptake of uPAR-PAI1 complexes (6, 11). Lipoprotein accumulation via macrophage VLDL R is instrumental in promoting the formation of atherosclerotic plaques (12). In the nervous system, VLDL R and ApoE R2 interactions with Reelin are critical for neuronal migration and positioning in the developing brain (13). VLDL R also functions in adult hippocampal synapse maturation, synaptic plasticity, and memory formation (14, 15). The ECD of mouse VLDL R shares 95% aa sequence identity with human and rat VLDL R. Within shared regions, mouse VLDL R shares 55% and 53% aa sequence identity with ApoE R2 and LDL R, respectively.

  • References:
    1. Qiu, S. et al. (2006) Neurobiol. Learn. Mem. 85:16.
    2. May, P. et al. (2005) Cell. Mol. Life Sci. 62:2325.
    3. Gafvels, M.E. et al. (1994) Endocrinology 135:387.
    4. Oka, K. et al. (1994) Eur. J. Biochem. 224:975.
    5. Wyne, K.L. et al. (1996) Arterioscler. Thromb. Vasc. Biol. 16:407.
    6. Argraves, K.M. et al. (1995) J. Biol. Chem. 270:26550.
    7. Goudriaan, J.R. et al. (2001) Arterioscler. Thromb. Vasc. Biol. 21:1488.
    8. Obunike, J.C. et al. (2001) J. Biol. Chem. 276:8934.
    9. Yagyu, H. et al. (2002) J. Biol. Chem. 277:10037.
    10. Goudriaan, J.R. et al. (2004) J. Lipid Res. 45:1475.
    11. Webb, D.J. et al. (1999) J. Biol. Chem. 274:7412.
    12. van Eck, M. et al. (2005) Atherosclerosis 183:230.
    13. Jossin, Y. et al. (2004) J. Neurosci. 24:514.
    14. Niu, S. et al. (2004) Neuron 41:71.
    15. Weeber, E.J. et al. (2002) J. Biol. Chem. 277:39944.
  • Long Name:
    Very Low Density Lipoprotein Receptor
  • Entrez Gene IDs:
    7436 (Human); 22359 (Mouse)
  • Alternate Names:
    CARMQ1; CHRMQ1; FLJ35024; very low density lipoprotein receptor; very low-density lipoprotein receptor; VLDL R; VLDL receptor; VLDLR; VLDL-R; VLDLRCH
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
Filter your results:

Species
Applications
Sample Type
  1. Pericyte loss influences Alzheimer-like neurodegeneration in mice.
    Authors: Sagare A, Bell R, Zhao Z, Ma Q, Winkler E, Ramanathan A, Zlokovic B
    Nat Commun, 2013;4(0):2932.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Blocking
  2. miR-200c regulates FGFR-dependent epithelial proliferation via Vldlr during submandibular gland branching morphogenesis.
    Authors: Rebustini IT, Hayashi T, Reynolds AD
    Development, 2012;139(1):191-202.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  3. Exercise during pregnancy mitigates Alzheimer-like pathology in mouse offspring.
    Authors: Herring A, Donath A, Yarmolenko M, Uslar E, Conzen C, Kanakis D, Bosma C, Worm K, Paulus W, Keyvani K
    FASEB J., 2012;26(1):117-28.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Smooth muscle-endothelial cell communication activates Reelin signaling and regulates lymphatic vessel formation.
    J. Cell Biol., 2012;197(6):837-49.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Expression of VLDLR in the retina and evolution of subretinal neovascularization in the knockout mouse model's retinal angiomatous proliferation.
    Authors: Hu W, Jiang A, Liang J, Meng H, Chang B, Gao H, Qiao X
    Invest. Ophthalmol. Vis. Sci., 2008;49(1):407-15.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  6. Bovine lactoferrin inhibits Japanese encephalitis virus by binding to heparan sulfate and receptor for low density lipoprotein.
    Authors: Chien YJ, Chen WJ, Hsu WL, Chiou SS
    Virology, 2008;379(1):143-51.
    Species: Hamster
    Sample Type: Whole Cells
    Application: Neut
  7. apoE isoform-specific disruption of amyloid beta peptide clearance from mouse brain.
    Authors: Deane R, Sagare A, Hamm K, Parisi M, Lane S, Finn MB, Holtzman DM, Zlokovic BV
    J. Clin. Invest., 2008;118(12):4002-13.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG HRP-conjugated Antibody

WB HAF017 15  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Antibody

WB AF109 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG VisUCyte HRP Polymer

IHC VC004  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse VLDL R Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information