VLDL R in Mouse Embryo. VLDL R was detected in immersion fixed frozen sections of mouse embryo using Mouse VLDL R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2258) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
VLDL R in Mouse Embryo. VLDL R was detected in immersion fixed frozen sections of mouse embryo using Mouse VLDL R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2258) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VLDL R
VLDL R is a 105 kDa type I integral membrane protein that belongs to the LDL receptor family. It plays a significant role in lipid metabolism and in nervous system development and function (1, 2). Mouse VLDL R has a 770 amino acid (aa) extracellular domain (ECD) and a 54 aa cytoplasmic region. The ECD contains eight LDLR class A repeats, three EGF-like repeats, six LDLR class B repeats, and a juxtamembrane region that is rich in O-linked glycosylation (3, 4). The cytoplasmic domain contains one NPXY internalization motif. VLDL R is predominantly expressed in striated muscle, adipose tissue, brain, and endothelial cells lining capillaries and small arterioles (3-6). VLDL R participates in the tissue uptake of fatty acids from plasma by mediating the internalization of ApoE-containing lipoparticles (i.e. VLDL, beta ‑VLDL, and chylomicron remnants) (5, 7). VLDL R binds and internalizes lipoprotein lipase (LPL) and mediates its transport from the basolateral to the lumenal face of endothelial cells (6, 8). VLDL R knockout mice are characterized by reduced LPL activity, reduced serum triglyceride clearance, and a resistance to developing obesity (7, 9, 10). VLDL R influences breast cancer cell motility by mediating the uptake of uPAR-PAI1 complexes (6, 11). Lipoprotein accumulation via macrophage VLDL R is instrumental in promoting the formation of atherosclerotic plaques (12). In the nervous system, VLDL R and ApoE R2 interactions with Reelin are critical for neuronal migration and positioning in the developing brain (13). VLDL R also functions in adult hippocampal synapse maturation, synaptic plasticity, and memory formation (14, 15). The ECD of mouse VLDL R shares 95% aa sequence identity with human and rat VLDL R. Within shared regions, mouse VLDL R shares 55% and 53% aa sequence identity with ApoE R2 and LDL R, respectively.
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Very Low Density Lipoprotein Receptor
Entrez Gene IDs:
7436 (Human); 22359 (Mouse)
CARMQ1; CHRMQ1; FLJ35024; very low density lipoprotein receptor; very low-density lipoprotein receptor; VLDL R; VLDL receptor; VLDLR; VLDL-R; VLDLRCH
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