Mouse VLDLR Antibody

Catalog # Availability Size / Price Qty
MAB2258
MAB2258-SP

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Citations (1)
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Mouse VLDLR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse VLDL R in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human ApoER2.
Source
Monoclonal Rat IgG2B Clone # 268724
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VLDL R
Thr25-Ala798
Accession # P98156
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse VLDL R (Catalog # 2258-VL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VLDLR

VLDL R is a 105 kDa type I integral membrane protein that belongs to the LDL receptor family. It plays a significant role in lipid metabolism and in nervous system development and function (1, 2). Mouse VLDL R has a 770 amino acid (aa) extracellular domain (ECD) and a 54 aa cytoplasmic region. The ECD contains eight LDLR class A repeats, three EGF-like repeats, six LDLR class B repeats, and a juxtamembrane region that is rich in O-linked glycosylation (3, 4). The cytoplasmic domain contains one NPXY internalization motif. VLDL R is predominantly expressed in striated muscle, adipose tissue, brain, and endothelial cells lining capillaries and small arterioles (3-6). VLDL R participates in the tissue uptake of fatty acids from plasma by mediating the internalization of ApoE-containing lipoparticles (i.e. VLDL, beta -VLDL, and chylomicron remnants) (5, 7). VLDL R binds and internalizes lipoprotein lipase (LPL) and mediates its transport from the basolateral to the lumenal face of endothelial cells (6, 8). VLDL R knockout mice are characterized by reduced LPL activity, reduced serum triglyceride clearance, and a resistance to developing obesity (7, 9, 10). VLDL R influences breast cancer cell motility by mediating the uptake of uPAR-PAI1 complexes (6, 11). Lipoprotein accumulation via macrophage VLDL R is instrumental in promoting the formation of atherosclerotic plaques (12). In the nervous system, VLDL R and ApoE R2 interactions with Reelin are critical for neuronal migration and positioning in the developing brain (13). VLDL R also functions in adult hippocampal synapse maturation, synaptic plasticity, and memory formation (14, 15). The ECD of mouse VLDL R shares 95% aa sequence identity with human and rat VLDL R. Within shared regions, mouse VLDL R shares 55% and 53% aa sequence identity with ApoE R2 and LDL R, respectively.

References
  1. Qiu, S. et al. (2006) Neurobiol. Learn. Mem. 85:16.
  2. May, P. et al. (2005) Cell. Mol. Life Sci. 62:2325.
  3. Gafvels, M.E. et al. (1994) Endocrinology 135:387.
  4. Oka, K. et al. (1994) Eur. J. Biochem. 224:975.
  5. Wyne, K.L. et al. (1996) Arterioscler. Thromb. Vasc. Biol. 16:407.
  6. Argraves, K.M. et al. (1995) J. Biol. Chem. 270:26550.
  7. Goudriaan, J.R. et al. (2001) Arterioscler. Thromb. Vasc. Biol. 21:1488.
  8. Obunike, J.C. et al. (2001) J. Biol. Chem. 276:8934.
  9. Yagyu, H. et al. (2002) J. Biol. Chem. 277:10037.
  10. Goudriaan, J.R. et al. (2004) J. Lipid Res. 45:1475.
  11. Webb, D.J. et al. (1999) J. Biol. Chem. 274:7412.
  12. van Eck, M. et al. (2005) Atherosclerosis 183:230.
  13. Jossin, Y. et al. (2004) J. Neurosci. 24:514.
  14. Niu, S. et al. (2004) Neuron 41:71.
  15. Weeber, E.J. et al. (2002) J. Biol. Chem. 277:39944.
Long Name
Very Low Density Lipoprotein Receptor
Entrez Gene IDs
7436 (Human); 22359 (Mouse)
Alternate Names
CARMQ1; CHRMQ1; FLJ35024; very low density lipoprotein receptor; very low-density lipoprotein receptor; VLDL R; VLDL receptor; VLDLR; VLDL-R; VLDLRCH

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Citation for Mouse VLDLR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. PCSK9 is not involved in the degradation of LDL receptors and BACE1 in the adult mouse brain.
    Authors: Liu M, Wu G, Baysarowich J
    J. Lipid Res., 2010-05-07;51(9):2611-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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